Identification and in vitro Characterization of Plant Growth-promoting Pseudomonas spp. Isolated from the Rhizosphere of Tomato (Lycopersicum esculentum) Plants in Kenya

Issifu, Muazu; Songoro, Edinah K.; Samuel, Niyomukiza; Ateka, Elijah; Onguso, Justus M.; Ngumi, Victoria Wambui

doi:10.13189/ujar.2022.100608

Cited by 3 publications

(4 citation statements)

References 44 publications

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“…Microbes are vital to plant growth like water and sunlight because they provide mineral nutrients, cycling, and nutrients from the biological system (Chhetri et al., 2022); (Koskey et al., 2021). Low nitrogen, phosphorus, and potassium in natural or artificial mediums (soil or soilless) affect plant growth, productivity, and quality (Issifu et al., 2022a). Scientists are interested in PGPB strains because they fix nitrogen, dissolve phosphorus, and mobilize potassium for plant growth.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the isolates showed varied levels of nitrogenase and phosphatase activity. Various nitrogen‐fixing, phosphate, and potassium‐solubilizing Pseudomonas bacteria have been isolated from tomato rhizosphere (Helal et al., 2022; Issifu et al., 2022a; Raji & Thangavelu, 2021; Yaghoubi Khanghahi et al., 2021). By generating nitrogenase, microorganisms convert atmospheric N 2 to plant‐available NH 3 .…”

Section: Discussionmentioning

confidence: 99%

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Synergistic effects of substrate inoculation with Pseudomonas strains on tomato phenology, yield, and selected human health‐related phytochemical compounds

Issifu,

Naitchede,

Ateka

et al. 2023

Agrosystems Geosci & Env

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Plant growth‐promoting rhizobacteria enhances nutritional availability during root colonization. Pseudomonas is the most popular bioinoculant due to its synergistic effects on plant development. This study evaluated the impact of four Pseudomonas strain substrate inoculations on tomato phenology, yield, and selected phytochemical components relevant to human health. The bacterial isolates have been characterized morphologically, biochemically, physiologically, and phylogenetically. Tomato plants on cocopeat substrate were inoculated individually with a bacterial suspension (optical density [OD]600 2.0). Controls included nitrogen–phosphate–potassium (NPK) fertilizer and tap water. The greenhouse experiment was laid out in a completely randomized design with five replications. Data were taken 30, 60, 90, and 120 days after inoculation. All four bacterial isolates could fix nitrogen, solubilize phosphate, and potassium and synthesize indole acetic acid in vitro. The 16S rRNA of the strains BF1P2‐6, BF2P1‐2, BF2P5‐1, and BF4P2‐5 were identified as Pseudomonas putida Y22, Pseudomonas taiwanensis GDLB‐32, Pseudomonas monteilii Bil‐NOR3_15, and Pseudomonas fluorescens Y5, respectively. The strains grew best at pH 7, NaCl 0.0%, and 35°C. Inoculated tomato plants tested over a period of 4 months in a greenhouse showed a significant increase in growth, leaf variables, and yield attributes. Pseudomonas‐inoculated plants were the highest in tannins (46.96 mg/100 g), followed by lycopene (45.57 mg/100 g) and Carotenoids (31.87 mg/100 g). The antioxidant activity of tomato fruit extracts showed the potential for free radicals scavenging activity against 2, 2‐diphenyl‐1‐picrylhydrazyl. Conditions such as pH and electrical conductivity for the cocopeat substrate were within acceptable limits. Bacterial strains and NPK increased cocopeat nitrogen, phosphate, and potassium bioavailability. This study reveals that Pseudomonas strains improve plant growth, yield, and health‐related phytochemical substances in tomato fruits, which could broaden their application in fruit and vegetable crops.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Synergistic effects of substrate inoculation with Pseudomonas strains on tomato phenology, yield, and selected human health‐related phytochemical compounds

Issifu,

Naitchede,

Ateka

et al. 2023

Agrosystems Geosci & Env

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“…In this salinity stress, the lowest reduction of leaf area was found in SAU Tomato 2 (17%), followed by BARI Tomato 14 and BARI Tomato 2 in approximately 27%, and the highest reduction was observed in Golden Purna (approximately 54%). the growth rate of tomato plants decreased as salt concentration increased [28] . The results indicated that plant growth and development are slowed by salt stress, resulting in a slower rate of leaf area expansion development.…”

Section: Leaf Areamentioning

confidence: 96%

Salinity tolerance in tomato genotypes at an early plant growth stage: Morphological and physiological responses

Choudhury,

Ali,

Sarker

et al. 2023

Trends Hortic.

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Salinity is a significant factor restricting plant development at various stages, resulting in lower yield and productivity. The current study was carried out to investigate and assess the tolerance of several tomato genotypes to salty conditions. Thirty (30) tomato genotypes were cultivated in pots and tested for salinity at three levels: 5 ds/m NaCl, 10 ds/m NaCl, and 15 ds/m NaCl, in comparison to the control (0 mM NaCl). Two weeks after treatment, several morphological and physiological parameters were measured. The effects of salt stress on tomato genotypes included a considerable reduction in leaf area, chlorophyll content, shoot and root length, shoot and root biomass, and relative water content. Different tomato genotypes responded differently to salinity severity score (SSS). Reduction of shoot dry weight (0.27 to 0.44) and leaf area (0.33 to 0.45) were positively correlated with SSS at moderate (10 ds/m) to higher (15 ds/m) salinity levels, respectively. Based on the experiment results, the genotypes BARI Tomato 4, BARI Tomato 14, BARI Tomato 15, SAU Tomato 2, AV0T0 1228, and NS 501 were found to be more salinity tolerant than other genotypes. The results showed that measuring shoot length, leaf area, and shoot fresh and dry weight was better for evaluating salinity stress and screening salt-tolerant tomato genotypes.

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“…Currently, the implementation of intensive agriculture has led to the development of diseases, nutrient deficits, soil degradation, loss of biodiversity, ero sion, among others (Pandey et al, 2019;Posada et al, 2021). One of the strategies to counteract this problem lies in the use of microorganisms with agricultural potential and among the most promising are plant growth promoting rhizobacteria (PGPR) and arbuscular mycorrhizal fungi (AMF) (Urgiles-Gómez et al, 2021); these microorganisms have the ability to minimize depen dence on chemical fertilizers and pesticides, thus optimizing soil fertility and quality (Zuluaga et al, 2020;Issifu et al, 2022), are also attributed with the ability to offer resistance to pathogens and environmental stress (Tabacchioni et al, 2021) and facilitate the stimulation of plant growth through mechanisms such as biological nitrogen fixation (BNF), phytohormone production, synthesis of antibiotics and organic acids related to plant growth promotion, phosphate solubiliza tion, siderophore production, cellulase and protease synthesis, among others (dos San tos et al, 2022;Mghazli et al, 2022;Sachman-Ruíz et al, 2022).…”

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confidence: 99%

Aplicación de técnicas moleculares en microbiología del suelo para la identificación de bacterias con potencial agrícola: una revisión y análisis bibliométrico

Dodino-Gutiérrez,

Santiago-Galvis,

Rabelo-Florez

et al. 2023

Rev. Colomb. Cienc. Hortic

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The excessive use of agrochemicals and poor agricultural practices have increased the negative effects on soil and crop biodiversity. In that sense, there is a need to identify potential bacteria by molecular techniques for sustainable agricultural production. The objective of this article was to develop a systematic and bibliometric mapping of the research carried out applying molecular techniques in soil microbiology for the identification of bacteria with agricultural potential. A search for research related to molecular techniques used for the identification of bacteria with agricultural potential was carried out in the Web of Science and Scopus databases, which were classified and analyzed by means of the R studio software. The origin, theoretical reference, bibliometric study and networks on the proposed topic were analyzed from the research obtained. A total of 527 researches related to molecular techniques used for the identification of bacteria with agricultural potential were reported, increasing by 52.75% in the last five years, with an annual growth rate of 17.4%, with India standing out as the country with the highest number of publications, contributing 25% of researches worldwide. Sequencing and PCR are the most common techniques to identify potential microorganisms, being Bacillus, Pseudomonas, Enterobacter and Acinetobacter the most frequent bacterial genera to be identified due to mechanisms used to favor sustainable agricultural production systems.

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Identification and in vitro Characterization of Plant Growth-promoting Pseudomonas spp. Isolated from the Rhizosphere of Tomato (Lycopersicum esculentum) Plants in Kenya

Cited by 3 publications

References 44 publications

Synergistic effects of substrate inoculation with Pseudomonas strains on tomato phenology, yield, and selected human health‐related phytochemical compounds

Synergistic effects of substrate inoculation with Pseudomonas strains on tomato phenology, yield, and selected human health‐related phytochemical compounds

Salinity tolerance in tomato genotypes at an early plant growth stage: Morphological and physiological responses

Aplicación de técnicas moleculares en microbiología del suelo para la identificación de bacterias con potencial agrícola: una revisión y análisis bibliométrico

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