Identification and Mapping of <i>Lr3</i> and a Linked Leaf Rust Resistance Gene in Durum Wheat

Herrera-Foessel, Sybil A.; Singh, Ravi P.; Espino, Julio Huerta; William, M.; Rosewarne, Garry M.; Djurle, Annika; Yuen, Jonathan

doi:10.2135/cropsci2006.10.0663

Cited by 47 publications

(26 citation statements)

References 29 publications

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“…While effective to BBG/BN, these mentioned genes are not effective to the predominant Pt races in bread wheat (14). Two additional resistance genes, known only in durum wheat, were also identified as effective to race BBG/BN: Lról in 'Guayacan INIA' and an undesignated gene in Camayo (7,8).…”

Section: Durum Wheat {Triticum Turgidum Subsp Durum) Is the Second Mmentioning

confidence: 99%

Lr72 Confers Resistance to Leaf Rust in Durum Wheat Cultivar Atil C2000

et al. 2014

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Section: Durum Wheat {Triticum Turgidum Subsp Durum) Is the Second Mmentioning

confidence: 99%

Lr72 Confers Resistance to Leaf Rust in Durum Wheat Cultivar Atil C2000

et al. 2014

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“…Some Lr genes known to be present in bread wheat such as Lr3 (Herrera‐Foessel et al. ) and Lr27 + Lr31 (Huerta‐Espino et al. ) were also found in durum wheat.…”

Section: Discussionmentioning

confidence: 99%

Sources of resistance to Indian pathotypes of Puccinia graminis tritici and P. triticina in durum wheat

et al. 2015

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Stem rust and leaf rust are important diseases affecting durum wheat production in India. Inheritance and extent of diversity in resistance were studied in five durum genotypes, viz. ʽB 662', ʽED 2398-A', ʽHG 110', ʽIWP 5019' and ʽLine 1172ʼ using Pgt pathotypes 40A and 117-6, and Pt pathotypes 12-2 and 104-2. F 2 and F 3 analyses showed that resistance was conferred by one or two genes in each line. In all, four genes for resistance to 40A, and eight each for pathotypes 117-6 and 12-2 were identified among the five genotypes, and three for resistance to 104-2 among B 662, ED 2398-A and IWP 5019 were indicated by tests of allelism. Although the gene identities are not known, at least some of them should be different from Sr2, Sr7b, Sr8a, Sr8b, Sr9e, Sr9g, Sr11, Sr12, Sr13, Sr14, Sr17, Sr23 and Sr28; and from Lr3, Lr14a, Lr23 and Lr27 + Lr31. These genotypes enrich the diversity of resistance to stem rust and leaf rust for durum wheat improvement.Key words: genetic diversity -Lr genes -resistance durability -Sr genes -Triticum turgidum ssp. durum India produces more than 90 million tonnes of wheat from an area of more than 25 million hectares, to which the contribution of durum wheat is about 5%. However, durum wheat has a special niche in the Indian wheat economy for at least two reasons: firstly, Indian durum is typically purchased by private traders at a premium price, mainly for processing high-value products; secondly, durum is preferred over bread wheat for several local food preparations. Durum and cultivated emmer are mainly grown in the central and southern parts, where leaf rust and stem rust are the major disease problems affecting wheat. Broadening the resistance base through the utilization of genetically diverse resistance sources is necessary to enhance the durability of resistance to meet ongoing threats from the continuously evolving rust pathogens. Relatively, little work has been done on the inheritance of rust resistance in durum, compared to bread wheat. However, studies conducted in different parts of the world indicate that durum generally shows resistance to Puccinia triticina (Pt) pathotypes virulent to bread wheat and vice versa

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“…triticina race BBG/BN with virulence to Lr72 , a widely-deployed gene in the CIMMYT durum germplasm, has led to severe epidemics in northwestern Mexico, from 2001 to 2003 [ 8 , 15 ]. Since then, genetic studies conducted at CIMMYT have led to the identification of effective resistance genes in modern durum wheat germplasm, including the linked genes Lr3 and Lr Camayo , both mapped to chromosome 6BL [ 16 ], the complementary gene pair Lr27+31 , located on chromosome arms 3BS and 4BS, respectively [ 17 ], Lr14a on chromosome 7BL [ 18 ] and the newly designated Lr61 on chromosome 6BS [ 19 ]. As the race BBG/BN continued to evolve, a new variant identified as BBG/BP acquired virulence to the complementary resistance genes Lr27+Lr31 in 2008 [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization and mapping of leaf rust resistance in four durum wheat cultivars

Kthiri¹,

Loladze²,

MacLachlan³

et al. 2018

PLoS ONE

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Widening the genetic basis of leaf rust resistance is a primary objective of the global durum wheat breeding effort at the International Wheat and Maize Improvement Center (CIMMYT). Breeding programs in North America are following suit, especially after the emergence of new races of Puccinia triticina such as BBG/BP and BBBQD in Mexico and the United States, respectively. This study was conducted to characterize and map previously undescribed genes for leaf rust resistance in durum wheat and to develop reliable molecular markers for marker-assisted breeding. Four recombinant inbred line (RIL) mapping populations derived from the resistance sources Amria, Byblos, Geromtel_3 and Tunsyr_2, which were crossed to the susceptible line ATRED #2, were evaluated for their reaction to the Mexican race BBG/BP of P. triticina. Genetic analyses of host reactions indicated that leaf rust resistance in these genotypes was based on major seedling resistance genes. Allelism tests among resistant parents supported that Amria and Byblos carried allelic or closely linked genes. The resistance in Geromtel_3 and Tunsyr_2 also appeared to be allelic. Bulked segregant analysis using the Infinium iSelect 90K single nucleotide polymorphism (SNP) array identified two genomic regions for leaf rust resistance; one on chromosome 6BS for Geromtel_3 and Tunsyr_2 and the other on chromosome 7BL for Amria and Byblos. Polymorphic SNPs identified within these regions were converted to kompetitive allele-specific PCR (KASP) assays and used to genotype the RIL populations. KASP markers usw215 and usw218 were the closest to the resistance genes in Geromtel_3 and Tunsyr_2, while usw260 was closely linked to the resistance genes in Amria and Byblos. DNA sequences associated with these SNP markers were anchored to the wild emmer wheat (WEW) reference sequence, which identified several candidate resistance genes. The molecular markers reported herein will be useful to effectively pyramid these resistance genes with other previously marked genes into adapted, elite durum wheat genotypes.

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Identification and Mapping of Lr3 and a Linked Leaf Rust Resistance Gene in Durum Wheat

Cited by 47 publications

References 29 publications

Lr72 Confers Resistance to Leaf Rust in Durum Wheat Cultivar Atil C2000

Lr72 Confers Resistance to Leaf Rust in Durum Wheat Cultivar Atil C2000

Sources of resistance to Indian pathotypes of Puccinia graminis tritici and P. triticina in durum wheat

Characterization and mapping of leaf rust resistance in four durum wheat cultivars

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