Identification and mapping of two powdery mildew resistance genes in Triticum boeoticum L.

Chhuneja, Parveen; Kumar, K. Ravi; Stirnweis, Daniel; Hurni, Severine; Keller, Beat; Dhaliwal, H. S.; Singh, Kuldeep

doi:10.1007/s00122-011-1768-4

Cited by 36 publications

(29 citation statements)

References 29 publications

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“…4). The first group is proximal to Xwmc525 , and includes NCA4 and NCAG11 [52], NCA6 [53], PmTb7A.1 [54] and Pm37 [55]. The second group is located in the Xwmc525 - Xgwm344 genetic interval.…”

Section: Discussionmentioning

confidence: 99%

“…The second group is located in the Xwmc525 - Xgwm344 genetic interval. Members of this group include the Pm1 [36], [37], Mlm2033 and Mlm80 from T. monococcum [35], PmTb7A.2 from T. boeoticum [54], PmU from T. urartu [56], MlAG1 2 from T. timopheevii [57], as well as MlIW72 [58], HSM1 [10], and MlIW172 (current study) from wild emmer. The MlIW72 was identified from wild emmer IW72 collected in Kokhav Hashahar, Israel [58].…”

Section: Discussionmentioning

confidence: 99%

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Fine Physical and Genetic Mapping of Powdery Mildew Resistance Gene MlIW172 Originating from Wild Emmer (Triticum dicoccoides)

et al. 2014

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Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most important wheat diseases in the world. In this study, a single dominant powdery mildew resistance gene MlIW172 was identified in the IW172 wild emmer accession and mapped to the distal region of chromosome arm 7AL (bin7AL-16-0.86-0.90) via molecular marker analysis. MlIW172 was closely linked with the RFLP probe Xpsr680-derived STS marker Xmag2185 and the EST markers BE405531 and BE637476. This suggested that MlIW172 might be allelic to the Pm1 locus or a new locus closely linked to Pm1. By screening genomic BAC library of durum wheat cv. Langdon and 7AL-specific BAC library of hexaploid wheat cv. Chinese Spring, and after analyzing genome scaffolds of Triticum urartu containing the marker sequences, additional markers were developed to construct a fine genetic linkage map on the MlIW172 locus region and to delineate the resistance gene within a 0.48 cM interval. Comparative genetics analyses using ESTs and RFLP probe sequences flanking the MlIW172 region against other grass species revealed a general co-linearity in this region with the orthologous genomic regions of rice chromosome 6, Brachypodium chromosome 1, and sorghum chromosome 10. However, orthologous resistance gene-like RGA sequences were only present in wheat and Brachypodium. The BAC contigs and sequence scaffolds that we have developed provide a framework for the physical mapping and map-based cloning of MlIW172.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Fine Physical and Genetic Mapping of Powdery Mildew Resistance Gene MlIW172 Originating from Wild Emmer (Triticum dicoccoides)

et al. 2014

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“…T. monococcum holds large genetic diversity in multiple traits, and has been found to be suitable for mining important genes useful for improving wheat and related Triticeae crops [5,6]. For example, a number of T. monococcum accessions exhibit strong resistance to powdery mildew, leaf rust and cereal cyst nematode [7][8][9][10]. Some disease resistance genes in T. monococcum were transferred to common wheat through marker-assisted selection [7,8].…”

Section: Introductionmentioning

confidence: 99%

“…For example, a number of T. monococcum accessions exhibit strong resistance to powdery mildew, leaf rust and cereal cyst nematode [7][8][9][10]. Some disease resistance genes in T. monococcum were transferred to common wheat through marker-assisted selection [7,8]. Puroiudoline and high-molecular-weight glutenin subunit genes in T. monococcum were also introgressed into common wheat to improve grain milling and processing qualities [11][12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Molecular genetic analysis of phosphomannomutase genes in Triticum monococcum

Zhang

et al. 2015

The Crop Journal

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In higher plants, phosphomannomutase (PMM) is essential for synthesizing the antioxidant ascorbic acid through the Smirnoff-Wheeler pathway. Previously, we characterized six PMM genes (TaPMM-A1, A2, B1, B2, D1 and D2) in common wheat (Triticum aestivum, AABBDD).Here, we report a molecular genetic analysis of PMM genes in Triticum monococcum (A m A m ), a diploid wheat species whose A m genome is closely related to the A genome of common wheat. Two distinct PMM genes, TmPMM-1 and TmPMM-2, were found in T. monococcum. The coding region of TmPMM-1 was intact and highly conserved. In contrast, two main TmPMM-2 alleles were identified, with TmPMM-2a possessing an intact coding sequence and TmPMM-2b being a pseudogene. The transcript level of TmPMM-2a was much higher than that of TmPMM-2b, and a bacterially expressed TmPMM-2a recombinant protein displayed relatively high PMM activity. In general, the total transcript level of PMM was substantially higher in accessions carrying TmPMM-1 and TmPMM-2a than those harboring TmPMM-1 and TmPMM-2b. However, total PMM protein and activity levels did not differ drastically between the two genotypes. This work provides new information on PMM genes in T. monococcum and expands our understanding on Triticeae PMM genes, which may aid further functional and applied studies of PMM in crop plants.

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“…2 . Both the genes were mapped on chromosome 7AL at a distance of ~48cM [33]. Both genes were effective individually as well in combination against the PM races in Europe and India.…”

Section: Introductionmentioning

confidence: 99%

Marker Assisted Transfer of Two Powdery Mildew Resistance Genes PmTb7A.1 and PmTb7A.2 from Triticum boeoticum (Boiss.) to Triticum aestivum (L.)

et al. 2015

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Powdery mildew (PM), caused by Blumeria graminis f. sp. tritici, is one of the important wheat diseases, worldwide. Two PM resistance genes, designated as PmTb7A.1 and PmTb7A.2, were identified in T. boeoticum acc. pau5088 and mapped on chromosome 7AL approximately 48cM apart. Two resistance gene analogue (RGA)-STS markers Ta7AL-4556232 and 7AL-4426363 were identified to be linked to the PmTb7A.1 and PmTb7A.2, at a distance of 0.6cM and 6.0cM, respectively. In the present study, following marker assisted selection (MAS), the two genes were transferred to T. aestivum using T. durum as bridging species. As many as 12,317 florets of F1 of the cross T. durum /T. boeoticum were pollinated with T. aestivum lines PBW343-IL and PBW621 to produce 61 and 65 seeds, respectively, of three-way F1. The resulting F1s of the cross T. durum/T. boeoticum//T. aestivum were screened with marker flanking both the PM resistance genes PmTb7A.1 and PmTb7A.2 (foreground selection) and the selected plants were backcrossed to generate BC1F1. Marker assisted selection was carried both in BC1F1 and the BC2F1 generations. Introgression of alien chromatin in BC2F1 plants varied from 15.4 - 62.9 percent. Out of more than 110 BC2F1 plants showing introgression for markers linked to the two PM resistance genes, 40 agronomically desirable plants were selected for background selection for the carrier chromosome to identify the plants with minimum of the alien introgression. Cytological analysis showed that most plants have chromosome number ranging from 40-42. The BC2F2 plants homozygous for the two genes have been identified. These will be crossed to generate lines combining both the PM resistance genes but with minimal of the alien introgression. The PM resistance gene PmTb7A.1 maps in a region very close to Sr22, a stem rust resistance gene effective against the race Ug99. Analysis of selected plants with markers linked to Sr22 showed introgression of Sr22 from T. boeoticum in several BC2F1 plants. Thus, in addition to PM resistance, these progeny might also carry resistance to stem rust race Ug99.

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Identification and mapping of two powdery mildew resistance genes in Triticum boeoticum L.

Cited by 36 publications

References 29 publications

Fine Physical and Genetic Mapping of Powdery Mildew Resistance Gene MlIW172 Originating from Wild Emmer (Triticum dicoccoides)

Fine Physical and Genetic Mapping of Powdery Mildew Resistance Gene MlIW172 Originating from Wild Emmer (Triticum dicoccoides)

Molecular genetic analysis of phosphomannomutase genes in Triticum monococcum

Marker Assisted Transfer of Two Powdery Mildew Resistance Genes PmTb7A.1 and PmTb7A.2 from Triticum boeoticum (Boiss.) to Triticum aestivum (L.)

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