Identification and measurement of staphylococcal enterotoxin M from<i>Staphylococcus aureus</i>isolate associated with staphylococcal food poisoning

Zhao, Yanying; Zhu, Anni; Tang, Junni; Tang, Cheng; Chen, Juan

doi:10.1111/lam.12751

Cited by 27 publications

(16 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This situation is mainly due to the lack of sensitive methods for detecting non-classic toxins. However, the number of immunological tests for SEI, steadily increasing in recent decades, including SEG [ 41 ] SEH [ 42 ], SEI [ 43 ], SEK [ 44 ], SEM [ 45 ] and SEQ [ 37 ], enabled the demonstration that new enterotoxins can also be a possible cause of SFP outbreaks. Therefore, the presence of SEI-encoding genes in the CoNS genome can raise concerns about their involvement in SFP.…”

Section: Discussionmentioning

confidence: 99%

Enterotoxigenic Potential of Coagulase-Negative Staphylococci from Ready-to-Eat Food

Chajęcka‐Wierzchowska

Gajewska

Wiśniewski

et al. 2020

Pathogens

View full text Add to dashboard Cite

Although coagulase-positive staphylococci are considered to be the main factor responsible for food poisoning, an increasing role for the coagulase-negative staphylococci in the production of enterotoxins has been observed in recent years. This study was conducted to assess the occurrence of genes responsible for the production of staphylococcal enterotoxins (SE), enterotoxin-like toxins (SEI) and toxic shock syndrome toxin-1 (TSST-1) in coagulase-negative staphylococci (CoNS) isolated from ready-to-eat food from bars and restaurants. One hundred and eighteen CoNS strains were tested using polymerase chain reaction (PCR) to five superantigenic toxin genes, including five different types of classical enterotoxins (sea, seb, sec, sed and see) and the toxic shock syndrome toxin-1 (tsst-1) as well as to supertoxin-like genes. PCR-positive isolates were then tested using immunoenzymatic methods (SET-RPLA, Vidas SET 2) for toxin expression. Out of 118 CoNS strains, the presence of staphylococcal enterotoxins was confirmed in 72% of them. The most frequently found enterotoxin-like genotype was ser, selu. Two of the tested strains had up to ten different enterotoxin genes in the genome at the same time. Although no production of enterotoxins was detected in the CoNS, which means that their possible role in the epidemiology of food-borne diseases is minimal, the data demonstrated that the toxigenic capacity of the CoNS should not be ignored, and that this group of microorganisms should be continuously monitored in food.

show abstract

Section: Discussionmentioning

confidence: 99%

Enterotoxigenic Potential of Coagulase-Negative Staphylococci from Ready-to-Eat Food

Chajęcka‐Wierzchowska

Gajewska

Wiśniewski

et al. 2020

Pathogens

View full text Add to dashboard Cite

show abstract

“…Highly sensitive SE detection methods also allow to get new insights in the pathogenesis of S. aureus and food safety control measures that need to mitigate risks of staphylococcal intoxications. The detection of both emerging and established toxins under different conditions, including strain based differences, even at levels below those causing food poisoning symptoms, could elucidate their potential role in SFP [33,34]. Perreira and co-workers described the existence of low-enterotoxin-producing staphylococci that could still produce sufficient amounts of enterotoxins in food to cause SFP, which demonstrates the interest of the low detection limits of a method [35].…”

Section: Discussionmentioning

confidence: 99%

Food-Borne Outbreak Investigation and Molecular Typing: High Diversity of Staphylococcus aureus Strains and Importance of Toxin Detection

Denayer

Delbrassinne

Nia

et al. 2017

Toxins

View full text Add to dashboard Cite

Staphylococcus aureus is an important aetiological agent of food intoxications in the European Union as it can cause gastro-enteritis through the production of various staphylococcal enterotoxins (SEs) in foods. Reported enterotoxin dose levels causing food-borne illness are scarce and varying. Three food poisoning outbreaks due to enterotoxin-producing S. aureus strains which occurred in 2013 in Belgium are described. The outbreaks occurred in an elderly home, at a barbecue event and in a kindergarten and involved 28, 18, and six cases, respectively. Various food leftovers contained coagulase positive staphylococci (CPS). Low levels of staphylococcal enterotoxins ranging between 0.015 ng/g and 0.019 ng/g for enterotoxin A (SEA), and corresponding to 0.132 ng/g for SEC were quantified in the food leftovers for two of the reported outbreaks. Molecular typing of human and food isolates using pulsed-field gel electrophoresis (PFGE) and enterotoxin gene typing, confirmed the link between patients and the suspected foodstuffs. This also demonstrated the high diversity of CPS isolates both in the cases and in healthy persons carrying enterotoxin genes encoding emetic SEs for which no detection methods currently exist. For one outbreak, the investigation pointed out to the food handler who transmitted the outbreak strain to the food. Tools to improve staphylococcal food poisoning (SFP) investigations are presented.

show abstract

“…The isolate from our case was only positive for SEM among the superantigens. In previous studies, SEM was not reported to have an association with human disease, with the exception of food-poisoning cases (Zhao et al, 2017). However, SEM's in vitro T-cell stimulating ability was found to be equal to that of SEC (Pan et al, 2007).…”

Section: Discussionmentioning

confidence: 84%

Staphylococcal scarlet fever associated with staphylococcal enterotoxin M in an elderly patient

Mun

Kim

Baek

et al. 2019

International Journal of Infectious Diseases

View full text Add to dashboard Cite

Keywords:Staphylococcal scarlet fever Staphylococcal enterotoxin M Staphylococcal enterotoxin-like toxin M A B S T R A C TStaphylococcal scarlet fever (SSF) is characterized by an exanthem without enanthem, bullae, or exfoliation, and is known to be related to Staphylococcus aureus toxins, especially superantigens. It has been reported in children and young adults. Herein, we report the first case of an elderly patient with SSF caused by staphylococcal enterotoxin M (SEM), associated with otitis externa. The patient presented with maculopapular rashes on both arms, thighs, and abdomen and with erythroderma on the face, ears, neck, chest, and back, all of which was followed by desquamation on the face, ears, and trunk. A culture of ear discharge grew methicillin susceptible S. aureus that was only positive for SEM among the superantigens tested.

show abstract

Identification and measurement of staphylococcal enterotoxin M fromStaphylococcus aureusisolate associated with staphylococcal food poisoning

Cited by 27 publications

References 22 publications

Enterotoxigenic Potential of Coagulase-Negative Staphylococci from Ready-to-Eat Food

Enterotoxigenic Potential of Coagulase-Negative Staphylococci from Ready-to-Eat Food

Food-Borne Outbreak Investigation and Molecular Typing: High Diversity of Staphylococcus aureus Strains and Importance of Toxin Detection

Staphylococcal scarlet fever associated with staphylococcal enterotoxin M in an elderly patient

Contact Info

Product

Resources

About