Identification and molecular characterization of novel LMW-m and -s glutenin genes, and a chimeric -m/-i glutenin gene in 1A chromosome of three diploid Triticum species

Cuesta, Susana; Álvarez, José Ramón Guzmán; Guzmán, Carlos

doi:10.1016/j.jcs.2017.01.010

Cited by 8 publications

(5 citation statements)

References 27 publications

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“…Also, T. urartu, as A-genome donor of common wheat, carried two new alleles Glu-3A.3c and Glu-3A.3d. In the present study, the number of allelic variants exceeded that found by Zhang et al (2013) in common wheat, or by Wang et al (2010), Ahmadpoor et al (2014), Ahmadi and Pour-Aboughadareh (2015), Luo et al (2015) and Cuesta et al (2017) in wild relatives of wheat. The development of allele-specific markers is based on the polymorphisms of nucleotide sequences among different alleles, and the relationship between markers and phenotypes needs to be established.…”

Section: Discussioncontrasting

confidence: 56%

“…longissima (Jiang et al 2008), Ae. tauschii (Xu et al 2010), T. urartu (Caballero et al 2008;Ahmadi and Pour-Aboughadareh 2015;Cuesta et al 2017), T. monococcum ) and T. boeoticum (Ahmadi and Pour-Aboughadareh 2015). Despite prior studies showing that the wild relatives of wheat had the large variation in glutenin and gliadin subunits, no work has been undertaken to evaluate the whole samples of wild wheat species.…”

Section: Introductionmentioning

confidence: 99%

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Molecular detection of glutenin and gliadin genes in the domesticated and wild relatives of wheat using allele-specific markers

Ahmadi

Pour-Aboughadareh

Fabriki-Ourang

et al. 2018

Cereal Research Communications

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Glutenin and gliadin subunits play a key role in flour processing quality by network formation in dough. Wild relatives of crops have served as a pool of genetic variation for decades. In this study, 180 accessions from 12 domesticated and wild relatives of wheat were characterized for the glutenin and gliadin genes with allele-specific molecular markers. A total of 24 alleles were detected for the Glu-A3 and Gli-2A loci, which out of 19 amplified products identified as new alleles. Analysis of molecular variance (AMOVA) indicated that 90 and 65% of the genetic diversity were partitioned within two Aegilops and Triticum genera and their species, respectively. Furthermore, all glutenin and gliadin analyzed loci were polymorphic, indicating large genetic diversity within and between the wild species. Our results revealed that allelic variation of Glu-3A and Gli-As.2 is linked to genomic constitutions so that, Ae. caudata (C genome), Ae. neglecta (UM genome), Ae. umbellulata (U genome) and T. urartu (A u genome) harbor wide variation in the studied subunits. Hence, these species can be used in wheat quality breeding programs.

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Section: Discussioncontrasting

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Molecular detection of glutenin and gliadin genes in the domesticated and wild relatives of wheat using allele-specific markers

Ahmadi

Pour-Aboughadareh

Fabriki-Ourang

et al. 2018

Cereal Research Communications

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“…Finally, the investigation of wheat genetic resources to discover sources of new genes and alleles, and quality traits that are not common in the modern wheat pool is another important component of CIMMYT's research portfolio. Collections of bread and durum wheat landraces, wheat ancestors (including einkorn, emmer and spelt), and wild relatives (mainly Aegilops species) have been evaluated for traits related to wheat quality [38][39][40][41][42][43][44][45][46] . In these collections, novel genetic variability and promising genotypes with atypical phenotypic characteristics have been identified and can be considered as valuable parent materials to be exploited in breeding programs targeting improvement of quality traits and widening the range of the end-uses for wheat grain.…”

Section: Wheat Grain Quality Research Activitiesmentioning

confidence: 99%

Genetic improvement of wheat grain quality at CIMMYT

Guzmán

Ammar

Govindan

et al. 2019

Front. Agr. Sci. Eng.

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The International Maize and Wheat Improvement Center (CIMMYT) is the global leader in publiclyfunded maize and wheat research and in farming systems based on these crops. CIMMYT leads the Global Wheat Program (GWP), which includes some of the largest wheat breeding programs in the world. The GWP has been successful in developing wheat germplasm that is used extensively worldwide. Wheat quality improvement is a central component of all the breeding efforts at CIMMYT and the Wheat Chemistry and Quality Laboratory represents an integral part of the breeding programs. Wheat quality is addressed at CIMMYT over the full range of this very wide and variable concept with milling, processing, end-use and nutritional quality targeted. Wheat progenitors and advanced lines developed by the breeders are assessed for diverse quality attributes, with the aim of identifying those that fulfill the requirements in terms of milling, processing, end-use and nutritional quality in different target regions. Significant research is conducted to make more efficient the integration of wheat quality traits in the breeding programs. The main topics being addressed are (1) methodologies to analyze grain quality traits, (2) genetic control and environmental effects on quality traits, (3) characterization of genetic resources for quality improvement, and (4) diversifying grain properties for novel uses.

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“…Modifications in the number and distribution of cysteines in LMW-GS may lead to different gluten macropolymers (D'Ovidio and Masci 2004). Novel LMW-GS, such as chimera of LMW-i/-m, -s/-m, and -m/-m, as well as -m/-i, have been identified from Chinese Xinjiang winter wheat landraces (Hu et al 2020) and other wheat genetic resources (Li et al 2008a;Qin et al 2015;Cuesta et al 2017). Some of these chimeric genes showed an altered number and distribution of cysteines in comparison with their parental genes, thus modifying the structure of gluten polymers and the dough quality via intra-and intermolecular disulfide bonds (Li et al 2008b;Cuesta et al 2017;Qin et al 2015;Hu et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Characterization of novel LMW-i genes with nine cysteine residues from Chinese wheat landraces (Triticum aestivum L.) and analysis of their functional properties on dough mixing

et al. 2021

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The low-molecular-weight glutenin subunits (LMW-GS) with extra cysteine numbers have attracted great research interest for their potential quality value. In this study, 14 LMW-i type genes (YD1-YD14) were isolated from three types of Chinese wheat landraces; and nine of 14 genes (YD1-YD9) had nine cysteines, and the other five genes contained eight cysteines. Phylogenic analysis suggested that all 14 LMW-i genes were related to Glu-A3-1 variants Glu-A3-17/FJ 549934 and Glu-A3-15/FJ 549932. Six randomly selected genes, five genes including YD1 with nine cysteines and the remaining one with eight cysteines, were successfully expressed in bacteria as mature proteins with a molecular mass of ~ 46 kDa. These proteins were traced to corresponding seed storage proteins for having similar elution times in reverse phase high-performance liquid chromatography (RP-HPLC) profiles. Mass spectrometry verified that bacterial expressed protein pET-30a-YD1 was LMW-i. Dough mixing experiments for incorporation of 50 mg pET-30a-YD1 proteins into the base flour of weak gluten wheat cv. "Chuannong 16" indicated that the dough strength of mixing flours was noticeably weaker than that of the control, which was reflected by mixing parameters in 8-min curve width, peak width, peak height, mixing time, and right of peak slope. The results suggested that the LMW-i genes with nine cysteine residues in the present study contributed to inferior quality properties for wheat flour.

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Identification and molecular characterization of novel LMW-m and -s glutenin genes, and a chimeric -m/-i glutenin gene in 1A chromosome of three diploid Triticum species

Cited by 8 publications

References 27 publications

Molecular detection of glutenin and gliadin genes in the domesticated and wild relatives of wheat using allele-specific markers

Molecular detection of glutenin and gliadin genes in the domesticated and wild relatives of wheat using allele-specific markers

Genetic improvement of wheat grain quality at CIMMYT

Characterization of novel LMW-i genes with nine cysteine residues from Chinese wheat landraces (Triticum aestivum L.) and analysis of their functional properties on dough mixing

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