Identification and pathogenicity of Fusarium spp. in row crops in Nebraska

Parikh, Lipi; Kodati, Srikanth; Eskelson, M. J.; Adesemoye, Anthony O.

doi:10.1016/j.cropro.2018.02.019

Cited by 46 publications

(39 citation statements)

References 26 publications

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“…In this study, no introgressions affected resistance to either SCN or F. graminearum. Fusarium graminearum is a necrotophic ascomycete that infects soybean seed and seedlings (Broders et al, 2007;Parikh et al, 2018). Lack of pleiotropy in these interactions is not unexpected due to differences in pathogen biology and infection type between P. sojae and both SCN and F. graminearum.…”

Section: Discussionmentioning

confidence: 99%

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Phenotypic Characterization of a Major Quantitative Disease Resistance Locus for Partial Resistance to Phytophthora sojae

et al. 2019

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Major quantitative disease resistance loci (QDRLs) are rare in the Phytophthora sojae (Kaufmann and Gerdemann)–soybean [Glycine max (L). Merr.] pathosystem. A major QDRL on chromosome 18 (QDRL‐18) was identified in PI 427105B and PI 427106. QDRL‐18 represents a valuable resistance source for breeding programs. Thus, our objectives were to determine its isolate specificity and measure its effect on yield and resistance to both P. sojae and other soybean pathogens. We characterized near isogenic lines (NILs) developed from F7 recombinant inbred lines heterozygous at QDRL‐18; NILs represent introgressions from PI 427105B, PI 427106, and susceptible ‘OX20‐8’. The introgressions from PI 427105B and PI 427106 increased resistance to P. sojae by 11 to 20% and 35 to 40%, respectively, based on laboratory and greenhouse assays, and increased yield by 13 to 29% under disease conditions. The resistant introgression from PI 427105B was also effective against seven P. sojae isolates with no isolate specificity detected. Based on quantitative polymerase chain reaction assays, NILs with the susceptible introgression had significantly higher relative levels of P. sojae colonization 48 h after inoculation. No pleiotropic effects for resistance to either soybean cyst nematode or Fusarium graminearum were detected. This information improves soybean breeders’ ability to make informed decisions regarding the deployment of QDRL‐18 in their respective breeding programs.

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Section: Discussionmentioning

confidence: 99%

“…For example, the wheat (Triticum aestivum L.) gene Lr34 encodes an ATP-binding cassette transporter and provides partial resistance to three leaf pathogens: leaf rust (Puccinia triticina Erikss. in Ohio (Broders et al, 2007;Parikh et al, 2018). ), and powdery mildew [Blumeria graminis (DC) Speer] (Krattinger et al, 2009).…”

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confidence: 99%

Phenotypic Characterization of a Major Quantitative Disease Resistance Locus for Partial Resistance to Phytophthora sojae

et al. 2019

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“…Fusarium fujikuroi W343 (henceforth referred to as Ffuji) was originally isolated and identified from roots of infected wheat samples collected in Nebraska and maintained long term at −80 °C as agar plugs kept in 2 mL Nalgene cryogenic vials containing 1 mL potato dextrose broth (PDB) with 20% glycerol by Parikh et al (). In that study, DNA extraction and PCR amplification were conducted and the internal transcribed spacer (ITS) and β‐tubulin (BT) regions were sequenced.…”

Section: Methodsmentioning

confidence: 99%

“…In that study, DNA extraction and PCR amplification were conducted and the internal transcribed spacer (ITS) and β‐tubulin (BT) regions were sequenced. The sequences were accessioned (accession numbers for ITS and for BT) and details of the PCR amplification protocol and sequence analysis were reported by Parikh et al (). The strain was grown from agar plug cultures stored at −80 °C and used for the present study.…”

Section: Methodsmentioning

confidence: 99%

“…Natural endohyphal bacteria have also been reported to play important roles in the functional traits of their fungal hosts (Partida‐Martinez & Hertweck, ; Obasa et al , ). The present study therefore investigated two endohyphal Gram‐negative bacteria that were observed to be associated with mycelia of F. fujikuroi strain W343, isolated from wheat in Nebraska (Parikh et al , ), for roles in any of the functional traits of F. fujikuroi W343. The intracellular localization of the bacteria within the cytosol of the fungus was confirmed by confocal and scanning electron microscopy.…”

Section: Introductionmentioning

confidence: 99%

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Endohyphal bacteria associated with virulence, increased expression of fumonisin biosynthetic genes, and production of fumonisin and macroconidia in Fusarium fujikuroi W343

et al. 2019

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Until recently, Fusarium fujikuroi was widely described as a non‐producer or a producer of little B‐series fumonisin toxins despite having a complement of fumonisin biosynthetic genes similar to those in F. verticillioides and F. proliferatum. Although high and low fumonisin‐producing F. fujikuroi strains have been shown to induce typical disease symptoms, the relationship between fumonisin production and virulence in F. fujikuroi has not been fully established. Following recent reports on the roles of bacterial endosymbionts in influencing the biology of their fungal hosts, including virulence, reproduction and the production of secondary metabolites, this study investigated the association of two strains of bacteria of the genus Enterobacter with a F. fujikuroi strain isolated from wheat in Nebraska. Results demonstrated the intracellular localization of bacteria within the hyphae of the fungus. The association with the bacteria was also correlated with macroconidia production and higher levels of virulence in the fungus. Additionally, fumonisin production was consistently and significantly (α = 0.05) higher in the bacteria‐containing F. fujikuroi strain compared with the bacteria‐free strain. Furthermore, higher levels of fumonisin production by bacteria‐containing F. fujikuroi correlated with higher expression levels of the fumonisin biosynthetic genes fum1, fum8 and fum21 under fumonisin‐inducing and non‐inducing conditions. Expression of fum15, which encodes a P450 monooxygenase, was similarly observed to be significantly (α = 0.05) higher in the bacteria‐containing fungus. Analysis of transcripts also revealed significantly (α = 0.05) higher expression of the sexual and asexual development activator protein, VeA, in the bacteria‐containing F. fujikuroi under fumonisin‐inducing conditions.

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Control of phytopathogen organisms using bioactive compounds contained in orange wax

García‐Cruz,

Díaz‐Jiménez,

Enríquez‐Vara

et al. 2024

Biofuels Bioprod Bioref

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Orange wax (OW) is a waxy waste generated in the essential oil purification process (winterization). It has no commercial value. It is discarded or burnt, polluting the environment. The present study demonstrates the possibility of applying it to control phytopathogens. The ethanol‐soluble bioactive compounds of OW were identified and quantified. The effect of orange wax extracts on the growth inhibition of phytopathogenic fungi (Fusarium oxysporum and Colletotrichum gloeosporioides) and Spodoptera frugiperda larvae was evaluated. High‐performance liquid chromatography–mass spectrometry (HPLC‐MS) and gas chromatography–mass spectrometry (GC–MS) quantified 22 bioactive compounds representing 23% (w/w) of OW. Terpineol, limonene, and eremophilene were the most abundant bioactive substances in this material. The distortion of the radial growth of the fungal hyphae was measured in a potato dextrose agar (PDA), and all tested concentrations (6.3, 12.5, 25, 50, and 100 mg/mL) inhibited the radial expansion of C. gloeosporioides. In contrast, only the OW extracts at 50 and 100 mg mL−1 inhibited F. oxysporum. Spray treatments were performed using a Potter spray tower on third‐instar Spodoptera frugiperda larvae. Spraying OW extract at 50 mg mL−1 for 10 s caused the highest larval mortality. However, third‐instar larvae were attracted to consume corn leaves moistened with extract at lower concentrations. Up to 93% of the first‐instar larvae died after eating corn leaves moistened with the extract at 50 mg mL−1. As far as the authors are aware, this is the first scientific report about the use of bioactive compounds of orange wax to control fungi. Spodoptera frugiperda could form the basis for the manufacture of new biopesticides.

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Identification and pathogenicity of Fusarium spp. in row crops in Nebraska

Cited by 46 publications

References 26 publications

Phenotypic Characterization of a Major Quantitative Disease Resistance Locus for Partial Resistance to Phytophthora sojae

Phenotypic Characterization of a Major Quantitative Disease Resistance Locus for Partial Resistance to Phytophthora sojae

Endohyphal bacteria associated with virulence, increased expression of fumonisin biosynthetic genes, and production of fumonisin and macroconidia in Fusarium fujikuroi W343

Control of phytopathogen organisms using bioactive compounds contained in orange wax

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