Identification and pathogenicity of Lasiodiplodia theobromae causing dieback of table grapes in Peru

Rodríguez-Gálvez, E.; Maldonado, E.; Alves, Artur

doi:10.1007/s10658-014-0557-8

Cited by 53 publications

(33 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…; Rodríguez‐Gálvez et al . ), neutral or beneficial agents (Sanzani et al . ) to fruit yield and quality.…”

Section: Introductionmentioning

confidence: 99%

“…Like any other plant, the phyllosphere of table grape harbours diverse microbiota including fungi. Phyllosphere fungi, can either be phytopathogenic (Ottesen et al 2013;de Oliveira et al 2014;Rodr ıguez-G alvez et al 2015), neutral or beneficial agents (Sanzani et al 2014) to fruit yield and quality. Field phytopathogenic fungi on table grape surfaces can potentially remain on the harvested bunch going into the packhouse and subsequent packing and cold chain distribution influencing the final product quality at the end of the supply chain.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of fungal communities of developmental stages in table grape grown in the northern region of South Africa

et al. 2017

View full text Add to dashboard Cite

show abstract

“…; Rodríguez‐Gálvez et al . ), neutral or beneficial agents (Sanzani et al . ) to fruit yield and quality.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of fungal communities of developmental stages in table grape grown in the northern region of South Africa

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Lasiodiplodia (H18) strain (closely to L. pseudotheobromae ) also exhibited a high pectinolitic activity peaking at 143.5 in the YPD‐A medium. Differences in virulence among L. theobroma strains also have been reported in grape plants (Rodríguez‐Gálvez, Maldonado, & Alves, ). Different species of this genus also have been described as producers of cell wall‐degrading enzymes (Dekker, Vasconcelos, Barbosa, Giese, & Paccola‐Meirelles, ).…”

Section: Discussionmentioning

confidence: 54%

Bioprospecting for fungi with potential pathogenic activity on leaves of Agave tequilana Weber var. Azul

Campos-Rivero

Teyer

Cruz-Arguijo

et al. 2019

Journal of Phytopathology

View full text Add to dashboard Cite

Thirty different fungal strains were isolated from A. tequilana leaves showing disease symptoms such as wilt and curled leaves, black, red and chlorotic spots. Ten genera were identified and confirmed by using the LSU D1/D2 rDNA and ITS1‐5.8S‐ITS2 regions, mainly of the Ascomycota phylum, where the Lasiodiploidia and Neoscytalidium genera were the more (46.6%) abundant. The other genera identified were Cladosporium, Cytospora, Epicoccum, Flavodon, Lasiodiplodia, Myrmaecium, Neoscytalidium, Penicillium, Peniophora, Purpureocillium, Trametes and Fusarium. Five strains of Lasiodiplodia and one of Fusarium were selected based on their representativeness and pathogenic potential on Agaves. Pathogenic potential was analysed by both, an infection assay, evidenced as necrosis, and by pectinolytic activity. Specifically, necrosis infection assay was conducted by puncture (wounded) infection and by direct mycelium contact. In general, Lasiodiplodia strains exhibited different pathogenic profiles according to their necrosis percentages, regardless of the infection method used. Fusarium strain analysed also showed a high necrosis infection (> 99%). Pectinolytic activity used as an indirect measurement of pathogenesis presented a high Fusarium extract activity (peaking at 23.9 U). Lasiodiplodia strains exhibited up 6 times more enzymatic activity (peaking at 143.5) than Fusarium strain analysed. In addition, Agave leaf extracts used totally or partially as carbon source during fungal induction culture may induce different pathogenic activities in these strains. In general, the two pathogenicity assays implemented evidenced differences in the pathogenicity profile of these analysed strains.

show abstract

“…Among the other fungi, N. parvum and F. acuminatum isolated from healthy prickly acacia plants also caused similar lesions. Such lesions were also observed on other hosts following deliberate inoculation with the Botryosphaeriaceae and other fungi (Lynch et al 2013;Rodríguez-Gálvez et al 2015). L. pseudotheobromae caused significantly longer stem lesions compared to Cophinforma sp.…”

Section: Pigra) Collected From Northern Australia (Unpublished Findinmentioning

confidence: 66%

Investigation of the fungi associated with dieback of prickly acacia (Vachellia nilotica subsp. indica) in Northern Australia

Haque¹

View full text Add to dashboard Cite

Prickly acacia (Vachellia nilotica subsp. indica), one of the most harmful weeds of the Australian rangelands, has been occasionally observed displaying natural dieback symptoms since 1970's.More recently in 2010, a prominent widespread dieback event was observed among the plants growing around Richmond and Julia Creek in north-western Queensland. Affected plants were found with disease symptoms such as; ashy internal staining, defoliation, blackening of shoot tips through to widespread plant mortality. It was hypothesized that a pathogenic fungus/i could be implicated with this phenomenon and a potential for biological control of this invasive species might ensue.Fungi isolated from dieback-affected and healthy stands of prickly acacia were putatively identified by partial sequencing of the Internal Transcribed Spacer (ITS) region of genomic DNA.Botryosphaeriaceae was the best represented family of fungi associated with dieback. Among the Botryosphaeriaceae fungi, Cophinforma was found to be the most prevalent genus with 60% of the total isolates identified as Cophinforma spp. following BLAST searches. Cophinforma was also isolated from the healthy plants growing in the trial site at Richmond. Natural dieback on prickly acacia was previously observed in the surrounding areas. Phylogenetic analysis of the ITS sequences revealed the potential existence of new species of Cophinforma in Australia. Apart from Cophinforma sp., a range of other Botryosphaeriaceae and non-Botryosphaeriaceae fungi were isolated from both dieback-affected and healthy prickly acacia plants growing at different locations.Based on a seedling pathogenicity screening, a sub-set of 40 potentially pathogenic isolates across the range of species were selected to challenge the juvenile plants following a stem inoculation technique under glasshouse conditions. In the glasshouse trials, the test-isolates of Cophinforma sp.(isolated from both dieback-affected and healthy prickly acacia) and an isolate of Lasiodiplodia pseudotheobromae strain NT039 (isolated from dieback-affected Parkinsonia aculeata) consistently caused significant stem lesions, gummosis and leaf mortality. Cophinforma sp., Fusarium acuminatum, Neofusicoccum parvum and Tiarosporella graminis isolated from healthy prickly acacia also caused similar symptoms in glasshouse inoculation trials. The remaining species tested in the glasshouse trials were mostly non-pathogenic to the juvenile plants.In another study, given the observation that several fungi are often isolated from a single piece of dieback-affected stem, it was investigated whether typical dieback symptoms in prickly acacia could be replicated with combinations of different fungi. Therefore, the effect of inoculating with iii two isolates of Cophinforma sp. and one isolate of L. pseudotheobromae singly and in combination under glasshouse and field conditions was tested. In the glasshouse trial, significant stem lesions were observed with each of the test-isolates and their combinations. However, there was no signifi...

show abstract

Identification and pathogenicity of Lasiodiplodia theobromae causing dieback of table grapes in Peru

Cited by 53 publications

References 28 publications

Characterization of fungal communities of developmental stages in table grape grown in the northern region of South Africa

Characterization of fungal communities of developmental stages in table grape grown in the northern region of South Africa

Bioprospecting for fungi with potential pathogenic activity on leaves of Agave tequilana Weber var. Azul

Investigation of the fungi associated with dieback of prickly acacia (Vachellia nilotica subsp. indica) in Northern Australia

Contact Info

Product

Resources

About