Identification and phenotypic plasticity of Pseudanabaena catenata from the Svalbard archipelago

Abstract

Abstract:A filamentous benthic cyanobacteria, strain USMAC16, was isolated from the High Arctic Svalbard archipelago, Norway, and a combination of morphological, ultrastructural and molecular characterisation (16S rRNA gene sequence) used to identify to species level. Cell dimensions, thylakoid arrangement and apical cell shape are consistent with the Pseudanabaena genus description. The molecular characterisation of P. catenata gave 100% similarity with Pseudanabaena catenata SAG 1464-1, originally reported from Germany. Strain USMAC16 was cultured under a range of temperature and photoperiod conditions, in solid and liquid media, and harvested at exponential phase to examine its phenotypic plasticity. Under different culture conditions, we observed considerable variations in cell dimensions. The longest cell (5.91±0.13 μm) was observed at 15°C under 12:12 light:dark, and the widest cell (3.24±0.06 μm) at 4°C under 12:12 light: dark in liquid media. The study provides baseline data documenting the morphological variation of P. catenata in response to changing temperature regimes.

Isolation and evaluation of local microalgal isolates as feed for larval rearing of Crassostrea tulipa towards optimisation of mangrove oyster seed production in Ghana

“…Khan et al. (2017) reported that different strains of Pseudanabaena cultured under different illumination and temperature conditions exhibited varied widths (ESD) ranging from 1.31 to 3.24 µm. By virtue of the ESD of the microalgae cells, Rhodomonas sp.…”

Dominância de Cianobactérias na composição do Fitoplâncton em Reservatório de abastecimento no Semiárido Cearense

“…Para Buch & Bittencourt-Oliveira (2010), esses organismos são formadores de florações, e capazes de produzir hepatoxinas, neurotoxinas e dermatotoxinas, portanto, com efeitos diversos ao organismo, que segundo Calijuri;Alves & Santos (2006) Pseudanabaena é um gênero bem distribuido no mundo e bem comum em água doce, possui várias espécies planctônicas, ticoplanctônicas, metafíticas ou perifíticas, em reservatórios de águas oligo, meso e até eutróficas (Franceschini et al, 2010). A espécie Pseudanabaena catenata é um organismo de ambiente com temperatura média, porém pode se adaptar a temperaturas acima de 15 ºC, e a presença de maior incidência de luz pode provocar um efeito substancial sobre suas características morfológicas (Khan et al, 2017).…”