Identification and Probiotic Potency Screening of Cellulolytic Bacilli Isolated from Fecal Pellets and Gastrointestinal Tract of Nypha Worm (Namalycastis rhodochorde), West Kalimantan, Indonesia

Abstract

The bacterial isolates NrLtF1, NrLtF4, NrLtF5, and NrLtG2 isolated from fecal pellets and gastrointestinal tract of nypha worms (Namalycastis rhodochorde) have cellulolytic, proteolytic activity and produce organic acids. The four isolates have the potency to be developed as probiotics in nypha worm cultivation feed. This study aims to determine the probiotics potency and identify the species of NrLtF1, NrLtF4, NrLtF5, and NrLtG2 isolate based on 16srDNA sequence. The probiotic potency was carried out by the acid tolerance assays on distilled water and 0.3% acid bile media, and the antimicrobial testing against Escherichia coli (MF exp21.12). Bacterial identification was carried out by sequencing of 16sDNA sequence based on GeneBank data. The results showed that the bacterial isolates of NrLtF1, NrLtF4, NrLtF5, and NrLtG2 were able to grow on 0.3% distilled water and acid bile media. However, only the NrLtF4 and NrLtF5 inhibited E. coli (MF exp21.12) with halo zones 30 mm and 18 mm, respectively. Blasting results of the 16srDNA sequences showed that the NrLtF1, NrLtF4, NrLtF5, and NrLtG2 were closely related to Bacillus wiedmannii, Brevibacterium sediminis, Bacillus proteolyticus, and Bacillus paramycoides. The nypha worm bacterial isolates have the potency to be developed as probiotics in nypha worm culture.

Undesirable Microbial Growth from The Biodegradation Process of Nypa Palm Fronds Suplemented with Cellulolytic Bacteria Culture Isolated from Nypa Palm Worms (Namalycastis rhodochorde)

“…Bakteri lokal memiliki kemampuan mendegradasi pelepah nipah dapat digunakan untuk membantu proses penyediaan pakan dengan kandungan nutrisi yang dibutuhkan N. rhodochorde dapat menjadi solusi penyediaan pakan cacing nipah (Yanti et al, 2020;Setyawati et al, 2021). Namun, perlu dipelajari terkait mikrob lokal dari substrat alam seperti pelepah nipah untuk proses biodegradasi menggunakan bakteri selulolitik spesifik pada suatu fermentor.…”

Yeast diversity isolated from nypa palm leaf litter in the macrohabitat of the nypa palm worm (Namalycastis rhodochorde)

“…Yeast is commonly found in air, aquatic, and terrestrial, including in IOP Publishing doi:10.1088/1755-1315/1271/1/012008 2 mangrove sediment and estuary areas [2], plant tissues, leaves, flowers, fruits [3], soil, water, and fermented products [4]. Both of these microorganisms have been known to have several enzymes, such as the cellulase enzyme, which plays an essential role in breaking down organic carbon materials in nature [5,6]. Yeast degrades organic materials, such as nypa leaf litter, with the help of enzymes such as cellulase.…”