Identification and Quantification of Celery Allergens Using Fiber Optic Surface Plasmon Resonance PCR

Daems, Devin; Peeters, Bernd; Delport, Filip; Remans, Tony; Lammertyn, Jeroen; Spasić, Dragana

doi:10.3390/s17081754

Cited by 21 publications

(23 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The use of other molecular amplifiers to improve the LOD and reach the performances of best-in-class RDTs can also be investigated and optimized, such as the coupling of gold nanoparticles or nano-shell particles, often put in evidence in this field of research [42,43]. The use of more specific targets such as pLDH or other bioreceptors, such as DNA aptamers or molecularly imprinted polymers (MIPs) could also be investigated to improve biosensing responses [44].…”

Section: Discussion Of the Performances And Tunability Of The Lab-on-fiber Technologymentioning

confidence: 99%

PfHRP2 detection using plasmonic optrodes: performance analysis

Loyez

Wells

Hambye

et al. 2021

Malar J

View full text Add to dashboard Cite

Background Early malaria diagnosis and its profiling require the development of new sensing platforms enabling rapid and early analysis of parasites in blood or saliva, aside the widespread rapid diagnostic tests (RDTs). Methods This study shows the performance of a cost-effective optical fiber-based solution to target the presence of Plasmodium falciparum histidine-rich protein 2 (PfHRP2). Unclad multimode optical fiber probes are coated with a thin gold film to excite Surface Plasmon Resonance (SPR) yielding high sensitivity to bio-interactions between targets and bioreceptors grafted on the metal surface. Results Their performances are presented in laboratory conditions using PBS spiked with growing concentrations of purified target proteins and within in vitro cultures. Two probe configurations are studied through label-free detection and amplification using secondary antibodies to show the possibility to lower the intrisic limit of detection. Conclusions As malaria hits millions of people worldwide, the improvement and multiplexing of this optical fiber technique can be of great interest, especially for a future purpose of using multiple receptors on the fiber surface or several coated-nanoparticles as amplifiers.

show abstract

Section: Discussion Of the Performances And Tunability Of The Lab-on-fiber Technologymentioning

confidence: 99%

PfHRP2 detection using plasmonic optrodes: performance analysis

Loyez

Wells

Hambye

et al. 2021

Malar J

View full text Add to dashboard Cite

show abstract

“…To select one for establishing the competitive bioassay, we first tested their interaction with several thrombin concentrations (0, 62, 124, and 248 nM) in Buffer A , Buffer B , and Buffer C . This was done on a FO-SPR sensor, commercialized by FOx Biosystems (a platform based on an in-house developed technology [29,30]), by immobilizing thiol-modified aptamers on the FO-SPR probes (Fig. S2).…”

Section: Resultsmentioning

confidence: 99%

“…S1, ESM). The FO-SPR probes were manufactured and functionalized as described in Daems et al [30]. Briefly, FO-SPR probes, sputtered with a gold layer, were functionalized with the aptamer TBA 15 or TBA 28 through thiol binding (Fig.…”

Section: Development Of the Competitive Assay Buffer Screeningmentioning

confidence: 99%

DNA-only bioassay for simultaneous detection of proteins and nucleic acids

et al. 2021

Self Cite

View full text Add to dashboard Cite

Graphical abstract Testing multiple biomarkers, as opposed to one, has become a preferred approach for diagnosing many heterogeneous diseases, such as cancer and infectious diseases. However, numerous technologies, including gold standard ELISA and PCR, can detect only one type of biomarker, either protein or nucleic acid (NA), respectively. In this work, we report for the first time simultaneous detection of proteins and NAs in the same solution, using solely functional NA (FNA) molecules. In particular, we combined the thrombin binding aptamer (TBA) and the 10-23 RNA-cleaving DNA enzyme (DNAzyme) in a single aptazyme molecule (Aptazyme 1.15-3′ ), followed by extensive optimization of buffer composition, sequences and component ratios, to establish a competitive bioassay. Subsequently, to establish a multiplex bioassay, we designed a new aptazyme (Aptazyme 2.20-5′ ) by replacing the target recognition and substrate sequences within Aptazyme 1.15-3′ . This designing process included an in silico study, revealing the impact of the target recognition sequence on the aptazyme secondary structure and its catalytic activity. After proving the functionality of the new aptazyme in a singleplex bioassay, we demonstrated the capability of the two aptazymes to simultaneously detect thrombin and NA target, or two NA targets in a multiplex bioassay. High specificity in target detection was achieved with the limits of detection in the low nanomolar range, comparable to the singleplex bioassays. The presented results deepen the barely explored features of FNA for diagnosing multiple targets of different origins, adding an extra functionality to their catalogue. Supplementary Information The online version contains supplementary material available at 10.1007/s00216-021-03458-6.

show abstract

“…The copy numbers of the plasmids were used to detect the allergenic ingredients to 10 mg·protein·kg −1 in highly processed foods. Daems and coworkers developed an alternative to the traditional two-step molecular detection assays (i.e., qPCR followed by a high-resolution melting analysis) applying a fiber optic melting PCR method including mannitol dehydrogenase as a target gene for celery [ 148 ].…”

Section: Selected Instrumental Techniques and Their Applicationsmentioning

confidence: 99%

Selected Instrumental Techniques Applied in Food and Feed: Quality, Safety and Adulteration Analysis

Artavia

Cortés-Herrera

Granados-Chinchilla³

2021

Foods

View full text Add to dashboard Cite

This review presents an overall glance at selected instrumental analytical techniques and methods used in food analysis, focusing on their primary food science research applications. The methods described represent approaches that have already been developed or are currently being implemented in our laboratories. Some techniques are widespread and well known and hence we will focus only in very specific examples, whilst the relatively less common techniques applied in food science are covered in a wider fashion. We made a particular emphasis on the works published on this topic in the last five years. When appropriate, we referred the reader to specialized reports highlighting each technique’s principle and focused on said technologies’ applications in the food analysis field. Each example forwarded will consider the advantages and limitations of the application. Certain study cases will typify that several of the techniques mentioned are used simultaneously to resolve an issue, support novel data, or gather further information from the food sample.

show abstract

Identification and Quantification of Celery Allergens Using Fiber Optic Surface Plasmon Resonance PCR

Cited by 21 publications

References 28 publications

PfHRP2 detection using plasmonic optrodes: performance analysis

PfHRP2 detection using plasmonic optrodes: performance analysis

DNA-only bioassay for simultaneous detection of proteins and nucleic acids

Selected Instrumental Techniques Applied in Food and Feed: Quality, Safety and Adulteration Analysis

Contact Info

Product

Resources

About