Identification and quantitation of auxins in plants by liquid chromatography/electrospray ionization ion trap mass spectrometry

Abstract

Auxin is an important phylohormone, which regulates specific physiological responses such as division, elongation and differentiation of cells. A new method using liquid chromatography/electrospray ionization ion trap mass spectrometry (LC/ESI-ITMS) has been developed for identification and quantitation of four auxins. Under the optimum conditions, four auxins (indole-3-acetic acid, indole-3-propionic acid, indole-3-butyric acid and 1-naphthylacetic acid) were completely separated and quantitated within 7 min with a minimum detection limit of 8.0 ng mL(-1) with relative standard deviations lower than 5.0%. This method also has been applied to analysis of auxins in Chinese cabbage where, even with a complicated serious background perturbation due to the natural biological matrix, the mean recoveries ranged from 77.5% to 99.8%. Finally, we discuss the MS-relevant properties of the identified auxins in detail.

A liquid chromatography tandem mass spectrometry method for simultaneous determination of acid/alkaline phytohormones in grapes

“…As shown in Table 6, among the collected thirty samples, twenty two contained phytohormones (73.3% of incidence), ranging from 1.0 to 25.8 g kg −1 , which was consistent to the previous values reported by Dasharath et al (GA 3 : 0.05 (±4%) mg kg −1 ) in grapes [3], but higher than that in Chinese cabbage (no positive sample found) [18]. The three samples with the highest concentration levels of phytohormones (25.4 g kg −1 , 25.8 g kg −1 and 25.4 g kg −1 ) were all green grapes collected from Xinjiang province, China.…”

“…Practically, the most frequently used method for phytohormones analysis is liquid chromatography combined with different detectors since it combines high resolution with increasing sophisticated automation. However, HPLC analysis might suffer from interfering of the target HPLC-UV signals by matrix co-extractives, which render the separation time longer or the sample clean-up procedure more complex [1,18].…”

Simultaneous analysis of endogenetic and ectogenic plant hormones by pressurized capillary electrochromatography

“…Hormones were extracted by SPE as described in the literature [11] with some modifications. Briefly, 0.5 g of fresh Arabidopsis thaliana leaves was accurately weighed and ground into fine powder in the presence of liquid nitrogen.…”

“…To date, chromatographic separation techniques are still the main means for quantitative analysis of hormones. CE [5,6], GC [7], GC-MS [8,9], HPLC [6,10] and HPLC-MS [6,11,12] have all been reported to determine Aux and CKs.…”

Preparation of a multi‐hollow magnetic molecularly imprinted polymer for the selective enrichment of indolebutyric acid

“…To date, many methods have been developed for the determination of IBA, such as HPLC [8][9][10], LC-MS [11,12], GC-MS [6,13], and CE [14]. Although these methods have been successfully applied to the analysis of IBA in a variety of plants, they suffer from tedious procedure, time consumption or high cost [7].…”