Identification and selection of low-phosphate-tolerant germplasm in barley (<i>Hordeum vulgare</i> L.)

Ren, Ping; Ma, Xiao; Li, Baochun; Meng, Yali; Lai, Yong; Si, Erjing; Wang, Juncheng; Yao, Lirong; Yang, Ke; Shang, Xianwen; Wang, Huajun

doi:10.1080/00380768.2016.1223521

Cited by 9 publications

(9 citation statements)

References 64 publications

(63 reference statements)

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“…However, P use efficiency varied within a species. For example in Hordeum vulgare the expression of the gene HVPT5, that can be used to estimate phosphate use efficiency, was higher under low P availability in a tolerant accession, but its expression did not change in the sensitive accession ( Ren et al, 2016 ). Moreover, plants have inducible high affinity phosphate transporters and constitutive low affinity phosphate transporters encoded by Pht (phosphate transporter) gene family that ensure P uptake from the soil and distribution within different organelles of plant to sustain photosynthesis, respiration, and growth even under low P availability conditions ( López-Arredondo et al, 2014 ).…”

Section: Discussionmentioning

confidence: 99%

Phosphorous Application Improves Drought Tolerance of Phoebe zhennan

et al. 2017

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Phoebe zhennan (Gold Phoebe) is a threatened tree species in China and a valuable and important source of wood and bioactive compounds used in medicine. Apart from anthropogenic disturbances, several biotic constraints currently restrict its growth and development. However, little attention has been given to building adaptive strategies for its conservation by examining its morphological and physio-biochemical responses to drought stress, and the role of fertilizers on these responses. A randomized experimental design was used to investigate the effects of two levels of irrigation (well-watered and drought-stressed) and phosphorous (P) fertilization treatment (with and without P) to assess the morphological and physio-biochemical responses of P. zhennan seedlings to drought stress. In addition, we evaluated whether P application could mitigate the negative impacts of drought on plant growth and metabolism. Drought stress had a significant negative effect on the growth and metabolic processes of P. zhennan. Despite this, reduced leaf area, limited stomatal conductance, reduced transpiration rate, increased water use efficiency, enhanced antioxidant enzymes activities, and osmolytes accumulation suggested that the species has good adaptive strategies for tolerating drought stress. Application of P had a significant positive effect on root biomass, signifying its improved water extracting capacity from the soil. Moreover, P fertilization significantly increased leaf relative water content, net photosynthetic rate, and maximal quantum efficiency of PSII under drought stress conditions. This may be attributable to several factors, such as enhanced root biomass, decreased malondialdehyde content, and the up-regulation of chloroplast pigments, osmolytes, and nitrogenous compounds. However, P application had only a slight or negligible effect on the growth and metabolism of well-watered plants. In conclusion, P. zhennan has a strong capability for drought resistance, while P application facilitates and improves drought tolerance mostly through physio-biochemical adjustments, regardless of water availability. It is imperative to explore the underlying metabolic mechanisms and effects of different levels of P fertilization on P. zhennan under drought conditions in order to design appropriate conservation and management strategies for this species, which is at risk of extinction.

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Section: Discussionmentioning

confidence: 99%

Phosphorous Application Improves Drought Tolerance of Phoebe zhennan

et al. 2017

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“…Two barley genotypes (GN121, low-P-tolerant, and GN42, low-P-sensitive) were screened and used for this research (Ren et al, 2016 ). Hydroponic experiments were conducted from early January to late May in 2017 at Gansu Agricultural University (Lanzhou, China).…”

Section: Methodsmentioning

confidence: 99%

“…Previous studies have demonstrated that barley has unique adaptive mechanisms in response to abiotic stress (Zeng et al, 2014 ; Quan et al, 2016 ). In previous experiments, we have identified two barley genotypes with contrasting phosphorus efficiency, which provide excellent germplasms for subsequent molecular research (Ren et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanisms of Acclimatization to Phosphorus Starvation and Recovery Underlying Full-Length Transcriptome Profiling in Barley (Hordeum vulgare L.)

Ren

Meng

et al. 2018

Front. Plant Sci.

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A lack of phosphorus (P) in plants can severely constrain growth and development. Barley, one of the earliest domesticated crops, is extensively planted in poor soil around the world. To date, the molecular mechanisms of enduring low phosphorus, at the transcriptional level, in barley are still unclear. In the present study, two different barley genotypes (GN121 and GN42)—with contrasting phosphorus efficiency—were used to reveal adaptations to low phosphorus stress, at three time points, at the morphological, physiological, biochemical, and transcriptome level. GN121 growth was less affected by phosphorus starvation and recovery than that of GN42. The biomass and inorganic phosphorus concentration of GN121 and GN42 declined under the low phosphorus-induced stress and increased after recovery with normal phosphorus. However, the range of these parameters was higher in GN42 than in GN121. Subsequently, a more complete genome annotation was obtained by correcting with the data sequenced on Illumina HiSeq X 10 and PacBio RSII SMRT platform. A total of 6,182 and 5,270 differentially expressed genes (DEGs) were identified in GN121 and GN42, respectively. The majority of these DEGs were involved in phosphorus metabolism such as phospholipid degradation, hydrolysis of phosphoric enzymes, sucrose synthesis, phosphorylation/dephosphorylation and post-transcriptional regulation; expression of these genes was significantly different between GN121 and GN42. Specifically, six and seven DEGs were annotated as phosphorus transporters in roots and leaves, respectively. Furthermore, a putative model was constructed relying on key metabolic pathways related to phosphorus to illustrate the higher phosphorus efficiency of GN121 compared to GN42 under low phosphorus conditions. Results from this study provide a multi-transcriptome database and candidate genes for further study on phosphorus use efficiency (PUE).

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“…Two spring barley genotypes—GN121 (low-Pi-tolerant) and GN42 (low-Pi-sensitive)—were used (Ren et al, 2016 ). Seeds of GN121 and GN42 were obtained from Gansu Agricultural University (Lanzhou, China).…”

Section: Methodsmentioning

confidence: 99%

Dynamic Responses of Barley Root Succinyl-Proteome to Short-Term Phosphate Starvation and Recovery

Wang

et al. 2021

Front. Plant Sci.

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Barley (Hordeum vulgare L.)—a major cereal crop—has low Pi demand, which is a distinct advantage for studying the tolerance mechanisms of phosphorus deficiency. We surveyed dynamic protein succinylation events in barley roots in response to and recovery from Pi starvation by firstly evaluating the impact of Pi starvation in a Pi-tolerant (GN121) and Pi-sensitive (GN42) barley genotype exposed to long-term low Pi (40 d) followed by a high-Pi recovery for 10 d. An integrated proteomics approach involving label-free, immune-affinity enrichment, and high-resolution LC-MS/MS spectrometric analysis was then used to quantify succinylome and proteome in GN121 roots under short-term Pi starvation (6, 48 h) and Pi recovery (6, 48 h). We identified 2,840 succinylation sites (Ksuc) across 884 proteins; of which, 11 representative Ksuc motifs had the preferred amino acid residue (lysine). Furthermore, there were 81 differentially abundant succinylated proteins (DFASPs) from 119 succinylated sites, 83 DFASPs from 110 succinylated sites, 93 DFASPs from 139 succinylated sites, and 91 DFASPs from 123 succinylated sites during Pi starvation for 6 and 48 h and during Pi recovery for 6 and 48 h, respectively. Pi starvation enriched ribosome pathways, glycolysis, and RNA degradation. Pi recovery enriched the TCA cycle, glycolysis, and oxidative phosphorylation. Importantly, many of the DFASPs identified during Pi starvation were significantly overexpressed during Pi recovery. These results suggest that barley roots can regulate specific Ksuc site changes in response to Pi stress as well as specific metabolic processes. Resolving the metabolic pathways of succinylated protein regulation characteristics will improve phosphate acquisition and utilization efficiency in crops.

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Identification and selection of low-phosphate-tolerant germplasm in barley (Hordeum vulgare L.)

Cited by 9 publications

References 64 publications

Phosphorous Application Improves Drought Tolerance of Phoebe zhennan

Phosphorous Application Improves Drought Tolerance of Phoebe zhennan

Molecular Mechanisms of Acclimatization to Phosphorus Starvation and Recovery Underlying Full-Length Transcriptome Profiling in Barley (Hordeum vulgare L.)

Dynamic Responses of Barley Root Succinyl-Proteome to Short-Term Phosphate Starvation and Recovery

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