Identification and sequence analysis of the homologues of the herpes simplex virus type 1 glycoprotein H in Marek's disease virus and the herpesvirus of turkeys

Scott, Simon D.; Smith, G.; Ross, Norman; Binns, M. M.

doi:10.1099/0022-1317-74-6-1185

Cited by 23 publications

(14 citation statements)

References 48 publications

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“…In all human and animal herpesviruses studied to date, homologs of glycoprotein H (gH) and glycoprotein L (gL) have been found (9,14,15,17,18,21,22,26,32,34,35,40,41,43,44). These two envelope glycoproteins, which associate to form a gH-gL complex, have been implicated as key participants in fusion events that are critical to herpesvirus infection.…”

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The Human Herpesvirus 6 U100 Gene Product Is the Third Component of the gH-gL Glycoprotein Complex on the Viral Envelope

Mori

Akkapaiboon

Yang

et al. 2003

J Virol

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The human herpesvirus 6 (HHV-6) variant A U100 gene encodes the third component of the glycoprotein H (gH)-glycoprotein L (gL)-containing complex. Glycosidase digestion analysis showed that the U100 gene products are glycoproteins consisting of an 80-kDa protein with complex N-linked oligosaccharides and a 74-kDa protein with immature, high-mannose N-linked oligosaccharides. Based on these characteristics, we designated the U100 gene products glycoprotein Q (gQ). Only the 80-kDa form of gQ was coimmunoprecipitated with an anti-gH antibody, suggesting that the 80-kDa protein associates with the gH-gL complex in HHV-6-infected cells. Furthermore, the complex was detected in purified virions, suggesting that it may play an important role in viral entry.

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confidence: 99%

The Human Herpesvirus 6 U100 Gene Product Is the Third Component of the gH-gL Glycoprotein Complex on the Viral Envelope

Mori

Akkapaiboon

Yang

et al. 2003

J Virol

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“…The gH homologous genes are conserved downstream of the thymidine kinase (TK) homologous genes in various herpesviruses [8,21,25] including MDV1 and HVT [35]. As shown in Fig.…”

Section: Identification and Sequencing Of The Mdv2 Gh Genementioning

confidence: 99%

“…Because HSV-1 gH required glycoprotein L (gL) for proper posttranslational processing and transport to the cell surface [13]. Similarly, gH and gL associations have also been described in VZV [5], pseudorabies virus (PRV) [22], and equine herpesvirus type 1 (EHV-1) [38].In MDV1 and HVT genomes, the potential open reading frames (ORFs) which are homologous to the HSV-1 gH gene were reported [35]. The predicted primary polypeptide products of the MDV1 gH and HVT gH ORFs were 813 and 808 amino acids, respectively.…”

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Identification and DNA Sequence Analysis of the Marek's Disease Virus Serotype 2 Gene Homologous to the Herpes Simplex Virus Type 1 Glycoprotein H.

Shimojima

Jang

Ono

et al. 1997

The Journal of Veterinary Medical Science

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ABSTRACT. Marek's disease virus (MDV) serotype 2 (MDV2) gene homologous to the glycoprotein H (gH) gene of herpes simplex virus type 1 was identified and sequenced. The predicted region encoding for the MDV2 gH gene was 2436 nucleotide and the primary translation product was 812 amino acids with a molecular weight of 89.4 kDa. The protein encoded by MDV2 gH gene has a number of features characteristic of a membrane-associated glycoprotein. First, there are 9 potential N-linked glycosylation sites and 11 cysteine residues, and 6 of the sites and 8 of the residues were conserved among all of the three MDV serotypes. Second, this protein had Nterminal and C-terminal hydrophobic regions, which were a signal sequence and a transmembrane-anchor domain, respectively. From the northern blot analysis, it was suggested that a transcript encoding MDV2 gH and a poly-cistronic transcript encoding MDV2 thymidine kinase, gH, and possibly other genes of downstream on this strand existed. Alignment of the amino acid sequences of the gH homologues among the three MDV serotypes showed 57.5% (MDV1 and MDV2), 56.2% (MDV1 and HVT), and 50.1% (MDV2 and HVT) identities. [8,19,29]. Further, antibodies raised against gH homologues neutralize the infectivity of HSV-1, VZV and so on [7,18]. However, HSV-1 gH alone synthesized in a mammalian cell expression system or recombinant vaccinia virus-infected cells was slightly smaller than authentic HSV-1 gH and antigenically distinct from it, and not transported to the cell surface [6,10]. Because HSV-1 gH required glycoprotein L (gL) for proper posttranslational processing and transport to the cell surface [13]. Similarly, gH and gL associations have also been described in VZV [5], pseudorabies virus (PRV) [22], and equine herpesvirus type 1 (EHV-1) [38].In MDV1 and HVT genomes, the potential open reading frames (ORFs) which are homologous to the HSV-1 gH gene were reported [35]. The predicted primary polypeptide products of the MDV1 gH and HVT gH ORFs were 813 and 808 amino acids, respectively. Using polyclonal antiserum against MDV1 gL, gL coprecipitated with a molecular weight of 115 kDa which was predicted to be MDV1 gH from the size of the primary translational product. Further, MDV1 gL expressed alone was not properly processed to a mature form [41]. However, there is no evidence of the complex formation of gH and gL in MDV. Further it is unknown that the gH-gL complex is required

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“…In MDV genomes, eight glycoproteins, designated gB [33,45], gC [9,20], gD [7,17,48], gE [7,16,48], gH [36,38], gI [7,18,48], gK [32,43], and gL [47] have been found. Amino acid sequence analysis characterizes most of these proteins as type I membrane proteins, comprising an aminoterminal hydrophobic region as a putative signal sequence and a carboxy-terminal hydrophobic region as a transmembrane domain resulting in location of most of the amino-terminal part of the protein on the outside of the membrane.…”

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confidence: 99%

Identification and Structure of the Marek's Disease Virus Serotype 2 Glycoprotein M Gene: Comparison with Glycoprotein M Genes of Herpesviridae Family

Cai

Jang

Izumiya

et al. 1999

The Journal of Veterinary Medical Science

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ABSTRACT. We determined the nucleotide sequence of a portion of BamHI-C fragment of Marek's disease virus serotype 2 (MDV2) strain HPRS24 which was suspected to contain the homologue of the herpes simplex virus type 1 (HSV-1) gene UL10, encoding glycoprotein M (gM). An open reading frame whose translation product exhibited significant similarities to HSV-1 gM protein and respective proteins of other herpesviruses of 37.5% and 45.5% to 31.8%, respectively, was identified. A number of distinct transcriptional consensus sequences were found upstream of the first putative start codon of MDV2 UL10 protein. In transcriptional analysis, the gene was transcribed into an 1.5 kb RNA. The primary translation product comprises 424 amino acids with a predicted molecular weight of 46.9 kDa. The predicted MDV2 UL10 protein contains eight hydrophobic domains with sufficient length and hydrophobicity to span the lipid bilayer conserved in the genomes of all herpesviruses which have been sequenced so far. In the region located between the first and second hydrophobic domains, two potential N-linked glycosylation sites were presented. Interestingly, highly charged residues were abundantly possessed in the carboxy-terminal part of the MDV2 UL10 protein. By comparison of the amino acid sequence of the MDV2 UL10 gene with the homologues from other herpesviruses, the data might contribute for further evidence of the evolution of herpesviruses from a common progenitor and an ancient example of MDV2 belonging to the Alphaherpesvirinae subfamily. In addition, the existence of corresponding genes in human, mammalian, and avian herpesvirus genomes, suggests indirectly an important role for gM in the natural life cycle of the virus.-KEY WORDS: gM, MDV, MDV2, UL10.J. Vet. Med. Sci. 61(5): 503-511,1999 throughout the Herpesviridae family, including homologues of herpes simplex virus type 1 (HSV-1) gB, gH, gL, and gM, and so far, others, such as gC, gD, gE, gG, gI and gK, are found only in the Alphaherpesvirinae subfamily [39].

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Identification and sequence analysis of the homologues of the herpes simplex virus type 1 glycoprotein H in Marek's disease virus and the herpesvirus of turkeys

Cited by 23 publications

References 48 publications

The Human Herpesvirus 6 U100 Gene Product Is the Third Component of the gH-gL Glycoprotein Complex on the Viral Envelope

The Human Herpesvirus 6 U100 Gene Product Is the Third Component of the gH-gL Glycoprotein Complex on the Viral Envelope

Identification and DNA Sequence Analysis of the Marek's Disease Virus Serotype 2 Gene Homologous to the Herpes Simplex Virus Type 1 Glycoprotein H.

Identification and Structure of the Marek's Disease Virus Serotype 2 Glycoprotein M Gene: Comparison with Glycoprotein M Genes of Herpesviridae Family

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