Identification and transcriptional analysis of powdery mildew-induced barley genes

Jansen, Carin; Korell, Michael; Eckey, Christina; Biedenkopf, Dagmar; Kögel, Karl-Heinz

doi:10.1016/j.plantsci.2004.08.011

Cited by 24 publications

(17 citation statements)

References 31 publications

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“…This has been previously reported for wheat or barley (Bryngelsson and Green, 1989;Dudler et al, 1991;Rebmann et al, 1991a, b;Bryngelsson et al, 1994;Gregersen et al, 1997;Molina et al, 1999). Additionally, several spots represented candidates for Bghinduced gene expression because they had been identified previously by differential cDNA AFLP or subtractive hybridization for Bgh-induced genes (Eckey et al, 2004;Jansen et al, 2005). Several of them gave differential signals on the macroarrays.…”

Section: Differentially Expressed Genessupporting

confidence: 71%

“…This library might be enriched in salicylic-acid-induced transcripts but apparently not in pathogen-induced transcripts. Other reports showed that genes, which were induced during powdery mildew infection, were either not differentially expressed or down-regulated by salicylic acid or BTH (Besser et al, 2000;Hein et al, 2004;Jansen et al, 2005; Bettina Kah and G. L., University of Giessen, personal communication). Down-regulation of housekeeping or development-related genes might also represent physiological requirements for allocation of resources for defense (Zimmerli et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Only recently, several groups have identified an increasing number of barley genes that are differentially regulated in the interaction with Bgh (Gregersen et al, 1997;Caldo et al, 2004;Eckey et al, 2004;Hein et al, 2004;Jansen et al, 2005;Zierold et al, 2005). For most of the 94 differentially expressed candidates, gene expression did not strongly differ when either Bgh or Bgt was attacking.…”

Section: Discussionmentioning

confidence: 99%

“…Additional 192 clones were specifically selected for spotting, among them some that were already known to be pathogen responsive and some chemically induced genes. Respective cDNA libraries have been described previously (Besser et al, 2000;Hückelhoven et al, 2001b;Eckey et al, 2004;Jansen et al, 2005). The cDNAs were amplified by PCR using vectorspecific primers (forward 5 0 -GTTTTCCCAGTAAC-GACGACGTT-3 0 ; reverse 5 0 -CAGGAAACAGCTATGAC-CATG-3 0 ) and spotted in duplicate onto nylon membranes (Hybond N+, Amersham Bioscience, Freiburg, Germany) in a 3 Â 3 pattern using Microgrid II (Biorobotics, Cambrigde, UK) as described previously .…”

Section: Macroarray Preparationmentioning

confidence: 99%

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Macroarray expression analysis of barley susceptibility and nonhost resistance to Blumeria graminis

Eichmann

Biemelt

Schäfer

et al. 2006

Journal of Plant Physiology

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Section: Differentially Expressed Genessupporting

confidence: 71%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Macroarray Preparationmentioning

confidence: 99%

See 2 more Smart Citations

Macroarray expression analysis of barley susceptibility and nonhost resistance to Blumeria graminis

Eichmann

Biemelt

Schäfer

et al. 2006

Journal of Plant Physiology

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“…Subtractive suppression hybridization (SSH) is a valuable tool for molecular investigations of differentially expressed genes. It has been successfully used to isolate genes in rice (Qia et al 2005), carnation (Ok et al 2003), legume Sesbania rostrata (Schroeyers et al 2004), barley (Hedley et al 2000;Jansen et al 2005), wheat (Yao and Ni 2005), and maize (Van den Berg and Crampton 2004;Shi et al 2005). SSH and expressed sequence tag (EST) cloning can be used for identifying genes involved in the response to salt stress.…”

Section: Introductionmentioning

confidence: 99%

Identification of differentially expressed transcripts involved in the salt-stress response of Salsola ferganica by suppression subtractive hybridization

Guan

Jiang

Wang

et al. 2010

Plant Cell Tiss Organ Cult

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To understand the molecular and cellular mechanisms underlying the salt response and to isolate differentially expressed genes, we constructed a forward subtractive cDNA library from Salsola ferganica treated with 600 mM NaCl for different lengths of time (0, 4, 8, 12, 16, 24, 36 h) using the suppression subtractive hybridization (SSH) technique. This SSH library contained approximately 2,200 positive clones, with the insert size ranging from 250 to 700 bp. Eighty clones were selected for further study based on results from reverse Northern blotting and sequencing and, these cDNAs are the first reported expressed sequence tags (ESTs) of Salsola ferganica listed in GenBank. Among these 80 differentially expressed genes, 32 are genes with known function that display a high homology (between 68 and 100% identity) to known sequences, 47 have an unknown function, showing no homology with known sequences, and one is a novel gene that does not match any previously reported genes. Five cDNA fragments, sf3d8, sf6d8, sf4a8, sf8d6, and sf6c7, were randomly chosen from the 80 differentially expressed genes for reverse transcription-PCR analysis. The expression of sf8d6 was entirely induced by salt stress and that of sf3d8, sf6d8, sf4a8, and sf6c7 was increased from a relatively lower level. The isolated ESTs reported here may enable us to find new genes involved in salt tolerance in Salsola ferganica.

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A high-density consensus map of barley to compare the distribution of QTLs for partial resistance to Puccinia hordei and of defence gene homologues

et al. 2006

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A consensus map of barley was constructed based on three reference doubled haploid (DH) populations and three recombinant inbred line (RIL) populations. Several sets of microsatellites were used as bridge markers in the integration of those populations previously genotyped with RFLP or with AFLP markers. Another set of 61 genic microsatellites was mapped for the Wrst time using a newly developed Xuorescent labelling strategy, referred to as A/T labelling. The Wnal map contains 3,258 markers spanning 1,081 centiMorgans (cM) with an average distance between two adjacent loci of 0.33 cM. This is the highest density of markers reported for a barley genetic map to date. The consensus map was divided into 210 BINs of about 5 cM each in which were placed 19 quantitative trait loci (QTL) contributing to the partial resistance to barley leaf rust (Puccinia hordei Otth) in Wve of the integrated populations. Each parental barley combination segregated for diVerent sets of QTLs, with only few QTLs shared by any pair of cultivars. Defence gene homologues (DGH) were identiWed by tBlastx homology to known genes involved in the defence of plants against microbial pathogens. Sixty-three DGHs were located into the 210 BINs in order to identify candidate genes responsible for the QTL eVects. Eight BINs were cooccupied by a QTL and DGH(s). The positional candidates identiWed are receptor-like kinase, WIR1 homologues and several defence response genes like peroxidases, superoxide dismutase and thaumatin.

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Identification and transcriptional analysis of powdery mildew-induced barley genes

Cited by 24 publications

References 31 publications

Macroarray expression analysis of barley susceptibility and nonhost resistance to Blumeria graminis

Macroarray expression analysis of barley susceptibility and nonhost resistance to Blumeria graminis

Identification of differentially expressed transcripts involved in the salt-stress response of Salsola ferganica by suppression subtractive hybridization

A high-density consensus map of barley to compare the distribution of QTLs for partial resistance to Puccinia hordei and of defence gene homologues

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