Macroarray expression analysis of barley susceptibility and nonhost resistance to Blumeria graminis

Barley stripe mosaic virus-induced gene silencing (BSMV-IGS) as a tool for functional analysis of barley genes potentially involved in nonhost resistance

“…Plants inoculated with the barley transcriptome during host and nonhost interactions with powdery mildew. 10 Among the genes that were specifically upregulated in the Magnaporthe nonhost interaction and therefore might play a crucial role in NHR, several lipid transfer proteins, a cytochrome P450 and an ascorbate peroxidase were listed. 4 Functional characterization of these genes could be achieved by generating stable RNAi transformants, however, this is difficult and time-consuming in barley.…”

Accommodation of powdery mildew fungi in intact plant cells

“…A number of comparative gene expression profiling experiments have been conducted to dissect differential gene expression in compatible and incompatible interactions of plants with powdery mildew fungi (Caldo et al, 2004;Eckey et al, 2004;Gjetting et al, 2004;Zimmerli et al, 2004;Jansen et al, 2005;Zierold et al, 2005;Eichmann et al, 2006a). Mostly, differences in gene expression patterns between compatible and incompatible interactions are of quantitative nature Zierold et al, 2005;Eichmann et al, 2006a).…”

“…Mostly, differences in gene expression patterns between compatible and incompatible interactions are of quantitative nature Zierold et al, 2005;Eichmann et al, 2006a). Transcriptome analyses frequently unravel the elevated expression of ER-resident molecular chaperones and other secretion-related genes in response to powdery mildew attack (Gregersen et al, 1997;Eichmann et al, 2006a;Gjetting et al, 2007). Expression of the putative molecular chaperone calreticulin, for example, precedes or accompanies PR gene expression.…”

How can we exploit functional genomics approaches for understanding the nature of plant defences? Barley as a case study

“…Likewise the dotted filter array has been utilised for barley-Bgh interactions (Eichmann et al 2006;Gjetting et al 2007;Zierold et al 2005). The advantage of the dotted filter array compared to the microarray is that it is straightforward to add new sequences to the study as they are discovered.…”