Identification and typing of fish pathogenic species of the genus Tenacibaculum

Fernández‐Álvarez, Clara; Santos, Ysabel

doi:10.1007/s00253-018-9370-1

Cited by 52 publications

(37 citation statements)

References 87 publications

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“…T. maritimum might exhibit a viable but non-culturable (VBNC) state; increases in temperature and micronutrients such as iron have been reported to have roles in resuscitation of a Tenacibaculum isolate [ 33 ]. A lack of T. maritimum isolates may also be related to a lack of understanding of the exact requirements for selective growth; as media used for culturing T. maritimum [ 2 , 34 ] contain complex, undefined chemicals such as seawater [ 35 ] or yeast extract [ 36 , 37 ]. T. maritimum isolates may also be poorly competitive during isolation and other fast-growing bacteria may inhibit growth, as reported in Flavobacterium sp.…”

Section: Discussionmentioning

confidence: 99%

Application of Quantitative-PCR to Monitor Netpen Sites in British Columbia (Canada) for Tenacibaculum Species

et al. 2021

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Tenacibaculum are frequently detected from fish with tenacibaculosis at aquaculture sites; however, information on the ecology of these bacteria is sparse. Quantitative-PCR assays were used to detect T. maritimum and T. dicentrarchi at commercial Atlantic salmon (Salmo salar) netpen sites throughout several tenacibaculosis outbreaks. T. dicentrarchi and T. maritimum were identified in live fish, dead fish, other organisms associated with netpens, water samples and on inanimate substrates, which indicates a ubiquitous distribution around stocked netpen sites. Before an outbreak, T. dicentrarchi was found throughout the environment and from fish, and T. maritimum was infrequently identified. During an outbreak, increases in the bacterial load in were recorded and no differences were recorded after an outbreak supporting the observed recrudescence of mouthrot. More bacteria were recorded in the summer months, with more mortality events and antibiotic treatments, indicating that seasonality may influence tenacibaculosis; however, outbreaks occurred in both seasons. Relationships were identified between fish mortalities and antimicrobial use to water quality parameters (temperature, salinity, dissolved oxygen) (p < 0.05), but with low R2 values (<0.25), other variables are also involved. Furthermore, Tenacibaculum species appear to have a ubiquitous spatial and temporal distribution around stocked netpen sites, and with the potential to induce disease in Atlantic salmon, continued research is needed.

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Section: Discussionmentioning

confidence: 99%

Application of Quantitative-PCR to Monitor Netpen Sites in British Columbia (Canada) for Tenacibaculum Species

et al. 2021

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“…Since the initial discovery of T. maritimum, several other Tenacibaculum species have been isolated from different sources around the world, which led to an expansion of the genus to more than 30 different species (5,(7)(8)(9)(10)(11)(12)(13)(14). Many of the newly isolated species have been found in association with other marine hosts, such as fish and their eggs (e.g.…”

Section: Introductionmentioning

confidence: 99%

Insights into the microbiome of farmed Asian sea bass (Lates calcarifer) with symptoms of tenacibaculosis and description of Tenacibaculum singaporense sp. nov.

Miyake

Soh

Azman

et al. 2020

Antonie van Leeuwenhoek

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Outbreaks of diseases in farmed fish remain a recurring problem despite the development of vaccines and improved hygiene standards on aquaculture farms. One commonly observed bacterial disease in tropical aquaculture of the South-East Asian region is tenacibaculosis, which is attributed to members of the Bacteroidetes genus Tenacibaculum, most notably T. maritimum. The impact of tenacibaculosis on fish microbiota remains poorly understood. In this study, we analysed the microbiota of different tissue types of commercially reared Asian seabass (Lates calcarifer) that showed symptoms of tenacibaculosis and compared the microbial communities to those of healthy and experimentally infected fish that were exposed to diseased farm fish. The microbiota of diseased farm fish was dominated by Proteobacteria (relative abundance±standard deviation, 74.5%±22.8%) and Bacteroidetes (18.07%±21.7%), the latter mainly comprised by a high abundance of Tenacibaculum species (17.6%±20.7%).In healthy seabass Proteobacteria had also highest relative abundance (48.04%±0.02%), but Firmicutes (34.2%±0.02%) and Fusobacteria (12.0%±0.03%) were the next two major constituents. Experimentally infected fish developed lesions characteristic for tenacibaculosis, but the microbiota was primarily dominated by Proteobacteria (90.4%±0.2%) and Firmicutes (6.2%±0.1%). The relative abundance of Tenacibaculum species in experimentally infected fish was significantly lower than in the commercially reared diseased fish and revealed a higher prevalence of different Tenacibaculum species. One strain was isolated and is described here as sp. nov. Tenacibaculum singaporense TLL-A1 T (=DSM 106434 T , KCTC 62393 T ). The genome of T. singaporense was sequenced and compared to those of T. maritimum DSM 17995 T and the newly sequenced T. mesophilum DSM 13764 T .

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“…Many opportunistic pathogens for fish species are also included in the genus Tenacibaculum [64], such as Tenacibaculum discolor and Tenacibaculum gallaicum in the present work, which were observed as potentially pathogenic colonizers of microplastics with a clear preference for polymer types. These two species, isolated from sole (Solea senegalensis) and turbot (Psetta maxima) culture systems [65], have been identified as the causal agent of tenacibaculosis in fish [64]. It is noticed that the two species showed completely opposite dynamic trends in relative abundance throughout the incubation experiment.…”

Section: The Colonization and Succession Of Pathogens On Microplastics Varied With Different Substratesmentioning

confidence: 62%

“…Members of the genus detected on the surfaces of microplastics here, including Vibrio harveyi, Vibrio vulnificus, Vibrio fischeri, Vibrio splendidus, and some other Vibrio spp., are human and animal pathogens that have caused major pandemics and countless epidemics across the globe [61], as well as being the most common pathogens of fish and shellfish that have inflicted expensive losses on aquaculture enterprises [63]. Many opportunistic pathogens for fish species are also included in the genus Tenacibaculum [64], such as Tenacibaculum discolor and Tenacibaculum gallaicum in the present work, which were observed as potentially pathogenic colonizers of microplastics with a clear preference for polymer types. These two species, isolated from sole (Solea senegalensis) and turbot (Psetta maxima) culture systems [65], have been identified as the causal agent of tenacibaculosis in fish [64].…”

Section: The Colonization and Succession Of Pathogens On Microplastics Varied With Different Substratesmentioning

confidence: 65%

Assessing the Risks of Potential Bacterial Pathogens Attaching to Different Microplastics during the Summer–Autumn Period in a Mariculture Cage

et al. 2021

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As microplastic pollution continues to increase, an emerging threat is the potential for microplastics to act as novel substrates and/or carriers for pathogens. This is of particular concern for aquatic product safety given the growing evidence of microplastic ingestion by aquaculture species. However, the potential risks of pathogens associated with microplastics in mariculture remain poorly understood. Here, an in situ incubation experiment involving three typical microplastics including polyethylene terephthalate (PET), polyethylene (PE), and polypropylene (PP) was conducted during the summer–autumn period in a mariculture cage. The identification of potential pathogens based on the 16S rRNA gene amplicon sequencing and a custom-made database for pathogenic bacteria involved in aquatic environments, was performed to assess the risks of different microplastics attaching potential pathogens. The enrichment of pathogens was not observed in microplastic-associated communities when compared with free-living and particle-attached communities in surrounding seawater. Despite the lower relative abundance, pathogens showed different preferences for three microplastic substrates, of which PET was the most favored by pathogens, especially potentially pathogenic members of Vibrio, Tenacibaculum, and Escherichia. Moreover, the colonization of these pathogens on microplastics was strongly affected by environmental factors (e.g., temperature, nitrite). Our results provide insights into the ecological risks of microplastics in mariculture industry.

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Identification and typing of fish pathogenic species of the genus Tenacibaculum

Cited by 52 publications

References 87 publications

Application of Quantitative-PCR to Monitor Netpen Sites in British Columbia (Canada) for Tenacibaculum Species

Application of Quantitative-PCR to Monitor Netpen Sites in British Columbia (Canada) for Tenacibaculum Species

Insights into the microbiome of farmed Asian sea bass (Lates calcarifer) with symptoms of tenacibaculosis and description of Tenacibaculum singaporense sp. nov.

Assessing the Risks of Potential Bacterial Pathogens Attaching to Different Microplastics during the Summer–Autumn Period in a Mariculture Cage

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