Identification and verification of rhizosphere indicator microorganisms in tobacco root rot

Yao, Xiaoyuan; Huang, Kuo Chin; Zhao, Shiyuan; Cheng, Qian; Zhang, Shuting; Yang, Liang; Ding, Wei; Zhang, Yongqiang

doi:10.1002/agj2.20547

Cited by 10 publications

(4 citation statements)

References 42 publications

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“…and Streptomyces have been reported as suppressors of rhizosphere fungi that are pathogenic to other plant species, such as Fusariumoxysporum, Rhizoctonia solani, and Verticillium dahliae (CHAURASIA et al, 2005;ISLAM et al, 2012;CAO et al, 2016;SIEGEL-HERTZ et al, 2018;YAO et al 2020). These species were also reportedin this study, so future analyses should be carried out to evaluate the biological control capabilities of these bacterial members.…”

Section: Discussionmentioning

confidence: 99%

Rhizosphere bacterial and fungal communities of healthy and wilted pepper (Capsicum annuum L.) in an organic farming system

et al. 2023

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Rhizosphere microorganisms play an important role in the growth and health of plants. Around the world, diverse soil-borne pathogens attack Capsicum annuum causing significant damage and economic losses. This study determined whether the diversity and composition of microbial communities in the rhizosphere soil of C. annuum plants is significantly changed by wilt disease. We used the 16S rRNA gene for bacteria and the internal transcribed spacer region for fungi to characterize the rhizosphere microbiomes of healthy and wilted plants. The most abundant bacterial phyla were Proteobacteria and Gemmatimonadetes, while the most abundant fungal phyla were Ascomycota and Mucoromycota. The bacterial α-diversity did not show significant differences in richness and diversity, but did show a significant difference in evenness and dominance of species. Rare taxa were present in both healthy and wilted conditions with relative abundances < 1%. In the fungi, all evaluated estimators showed a significant reduction in the wilted condition. The β-diversity showed significant differences in the structure of bacterial and fungal communities, which were segregated according to plant health conditions. The same occurred when comparing the alpha and beta diversity of this study based on organic agriculture with that of other studies based on conventional agriculture. We observed a significant difference with estimators analyzed by segregating rhizosphere communities depending on the farming method used. Finally, the differential abundance analysis did not show significant results in the bacterial communities; however, in the fungal communities, Fusarium, Thanatephorus, Rhizopus, Curvularia, Cladosporium, and Alternaria were more abundant in the rhizosphere of wilted than healthy plants. Species from these genera have been previously reported as phytopathogens of several plants, including C. annuum.

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Section: Discussionmentioning

confidence: 99%

Rhizosphere bacterial and fungal communities of healthy and wilted pepper (Capsicum annuum L.) in an organic farming system

et al. 2023

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“…This cluster encompasses a broader range of genera, predominantly comprising PGPR species or indicators of soil health. Notable genera in this cluster include Bdellovibrio, Massilia, Phormidium, Phormidesmis, Acidovorax, Flavobacterium, Olivibacter, Arthrobacter, Kocuria, Noviherbaspirillum, and Nafulsella, previously mentioned as beneficial microorganisms; as well as Dyadobacter, known as a PGPR commonly present in the rhizosphere that enhances crop yield [81]; Streptomyces, commonly found in plant microbiomes with PGPR characteristics [82]; Rubrobacter, associated with potassium absorption and soil health [69,83]; Virgibacillus, housing halophytic PGPR species [84,85]; Pseudonocardia, a P-solubilizing bacterium and disease-suppressive bacterial agent [86,87]; Nocardioides, possessing PGPR capabilities to mitigate saline stress conditions [88,89]; and Promicromonospora, a PGPR producing gibberellins and mitigating the adverse effects of salinity and osmotic stress [90,91].…”

Section: Discussionmentioning

confidence: 99%

Fertilising Maize with Bio-Based Mineral Fertilisers Gives Similar Growth to Conventional Fertilisers and Does Not Alter Soil Microbiome

Barquero,

Cazador,

Ortiz-Liébana

et al. 2024

Agronomy

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The production of mineral fertilisers relies heavily on mineral deposits that are becoming depleted or is based on processes that are highly energy demanding. In this context, and in line with the circular economy and the European Green Deal, the recovery of nitrogen (N), phosphorus (P), and potassium (K) from organic wastes using chemical technologies is an important strategy to produce secondary raw materials for incorporation into mineral fertilisers, partially replacing the traditional sources of N, P, and K. However, there are very few studies on the agronomic and environmental effects of such substitution. The aim of this work was to evaluate plant growth under microcosm conditions and the effect on the soil microbiome of mineral fertilisers in which part of the N, P, or K content comes from bio-based materials (BBMFs), namely ash, struvite, and a patented chemical process. The crop was maize, and a metataxonomic approach was used to assess the effect on the soil microbiome. The BBMF treatments were compared with a control treated with a conventional mineral fertiliser. The conventional fertiliser performed significantly better than the bio-based fertilisers in terms of maize biomass production at the first sampling point 60 days after sowing (DAS), but at the last sampling point, 90 DAS, the BBMFs showed comparable or even better biomass production than the conventional one. This suggests that BBMFs may have a slightly slower nutrient release rate. The use of fertiliser, whether conventional or BBMF, resulted in a significant increase in microbiome biodiversity (Shannon index), while it did not affect species richness. Interestingly, the use of fertilisers modulated the composition of the bacterial community, increasing the abundance of beneficial bacterial taxa considered to be plant-growth-promoting bacteria, without significant differences between the conventional mineral fertilisers and the BBMFs. The predominance of PGPRs in the rhizosphere of crops when BBMFs are used could be part of the reason why BBMFs perform similarly or even better than conventional fertilisers, even if the rate of nutrient release is slower. This hypothesis will be tested in future field trials. Thus, BBMFs are an interesting option to make the food chain more sustainable.

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“…Little information is available about the genus Kaistobacter; however, some studies have reported species of this genus as being associated with active disease suppression in the rhizosphere of tobacco plants [21,37]. Moreover, genera including Bacillus, Rubrobacter, and Streptomyces have been reported as suppressors of rhizosphere fungi that are pathogenic to other plant species, such as Fusarium oxysporum, Rhizoctonia solani, and Verticillium dahliae [9,12,30,56,73]. These species were also found in this study, so future analyses should be carried out to evaluate the biological control capabilities of these bacterial members.…”

Section: Discussionmentioning

confidence: 99%

Microbial Community Analysis of Rhizosphere of Healthy and Wilted Pepper (Capsicum Annuum L.) in An Organic Farming System

González-Escobedo

Muñoz-Castellanos

Muñoz-Ramirez

et al. 2021

Preprint

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Rhizosphere microorganisms play an important role in the growth and health of plants. Around the world, diverse soil-borne pathogens attack Capsicum annuum causing significant damage and economic losses; however, very little is known about how rhizosphere microbial communities are altered by infestation with root pathogens. This work aimed to determine whether the diversity and structure of microbial communities in the rhizosphere soil of C. annuum plants is significantly changed by wilt disease. We used 16S rRNA for bacteria and the internal transcribed spacer region for fungi, to characterize the rhizosphere microbiomes of healthy and wilted plants. The most abundant bacterial phyla were Proteobacteria and Gemmatimonadetes, while the most abundant fungal phyla were Ascomycota and Mucoromycota. The bacterial α-diversity did not show significant differences in richness and diversity, but did show a significant difference in evenness and dominance of species. Rare taxa were present in both healthy and wilted conditions with relative abundances < 1%. In the fungi, all evaluated estimators showed a significant reduction in the wilted condition. The β-diversity showed significant differences in the structure of bacterial and fungal communities, which were segregated according to plant health condition. The differential abundance analysis did not show significant results in the bacterial communities; however, in the fungal communities, Rhizopus, Thanatephorus, Curvularia, Fusarium, Cladosporium, and Alternaria were more abundant in the rhizosphere of wilted than healthy plants. Species from these genera have been previously reported as phytopathogens of several plants, including in reports on individual species as disease agents in C. annuum.

show abstract

Identification and verification of rhizosphere indicator microorganisms in tobacco root rot

Cited by 10 publications

References 42 publications

Rhizosphere bacterial and fungal communities of healthy and wilted pepper (Capsicum annuum L.) in an organic farming system

Rhizosphere bacterial and fungal communities of healthy and wilted pepper (Capsicum annuum L.) in an organic farming system

Fertilising Maize with Bio-Based Mineral Fertilisers Gives Similar Growth to Conventional Fertilisers and Does Not Alter Soil Microbiome

Microbial Community Analysis of Rhizosphere of Healthy and Wilted Pepper (Capsicum Annuum L.) in An Organic Farming System

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