Identification by Suppression Subtractive Hybridization of
            <i>Frankia</i>
            Genes Induced under Nitrogen-Fixing Conditions

Yamaura, Masatoshi; Uchiumi, Toshiki; Higashi, Shiro; Abe, Mikiko; Kucho, Ken-ichi

doi:10.1128/aem.01813-09

Cited by 8 publications

(2 citation statements)

References 22 publications

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“…Unlike most α-rhizobia [ 78 ], P. phymatum contains a nifV homolog in the genome (upstream of nifB ), which is also highly induced during symbiosis. This gene encodes a homocitrate synthase that synthesizes homocitrate—a component of the Fe–Mo cofactor of the nitrogenase—which has been shown to be important in diazotrophs to reduce N 2 in free-living conditions [ 79 , 80 , 81 ]. The presence of nifV in P. phymatum may explain the ability of this bacterium to fix nitrogen in free-living conditions [ 4 ].…”

Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Paraburkholderia phymatum under Nitrogen Starvation and during Symbiosis with Phaseolus Vulgaris

Lardi

Liu

Purtschert

et al. 2017

Genes

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Paraburkholderia phymatum belongs to the β-subclass of proteobacteria. It has recently been shown to be able to nodulate and fix nitrogen in symbiosis with several mimosoid and papilionoid legumes. In contrast to the symbiosis of legumes with α-proteobacteria, very little is known about the molecular determinants underlying the successful establishment of this mutualistic relationship with β-proteobacteria. In this study, we performed an RNA-sequencing (RNA-seq) analysis of free-living P. phymatum growing under nitrogen-replete and -limited conditions, the latter partially mimicking the situation in nitrogen-deprived soils. Among the genes upregulated under nitrogen limitation, we found genes involved in exopolysaccharides production and in motility, two traits relevant for plant root infection. Next, RNA-seq data of P. phymatum grown under free-living conditions and from symbiotic root nodules of Phaseolus vulgaris (common bean) were generated and compared. Among the genes highly upregulated during symbiosis, we identified—besides the nif gene cluster—an operon encoding a potential cytochrome o ubiquinol oxidase (Bphy_3646-49). Bean root nodules induced by a cyoB mutant strain showed reduced nitrogenase and nitrogen fixation abilities, suggesting an important role of the cytochrome for respiration inside the nodule. The analysis of mutant strains for the RNA polymerase transcription factor RpoN (σ54) and its activator NifA indicated that—similar to the situation in α-rhizobia—P. phymatum RpoN and NifA are key regulators during symbiosis with P. vulgaris.

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Section: Discussionmentioning

confidence: 99%

Transcriptome Analysis of Paraburkholderia phymatum under Nitrogen Starvation and during Symbiosis with Phaseolus Vulgaris

Lardi

Liu

Purtschert

et al. 2017

Genes

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“…Most extensively studied nitrogen fixation by actinobacteria is Frankia, which lives in symbiotic association with dicotyledons. Almost 24 genera belonging to 8 families are infected with symbiosis and are called actinorhizal plants and form nitrogen-fixing root nodules in their roots (Yamaura et al 2010). Apart from the most commonly studied Frankia N fixation, a thermophilic actinobacteria Streptomyces thermoautotrophicus isolated from charcoal pile at 65 C can fix atmospheric nitrogen.…”

Section: Atmospheric Nitrogen Fixationmentioning

confidence: 99%

Role of Actinomycete-Mediated Nanosystem in Agriculture

Subramanian

Muniraj

Uthandi

2016

Plant Growth Promoting Actinobacteria

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Actinobacteria are a group of microorganisms sharing the common behaviour of both bacteria and fungi known to play a multifunctional role in agricultural production systems. The major functions include the production of a wide array of growth-promoting compounds and metabolites including antibiotics that provide the host plants to withstand both biotic and abiotic stress conditions. Consequently, actinobacteria are often employed as a biocontrol agent (BCA) against dreadful plant pathogens. Further, actinobacteria colonized host plants and elute growth-promoting substances that assist in favouring stimulated growth of plants even under harsh environmental conditions such as nutrient deficiencies, drought, salinity and heavy metal contaminated soils. Several actinobacteria are involved in the nutrient solubilization and mobilization particularly phosphates and iron besides facilitating as helper bacteria in mycorrhizal symbiosis and biological nitrogen fixation. These groups of organisms also are responsible for the production of a volatile compound called "geosmin" which often referred as a biological indicator of soil fertility. Recently, large volume of research reports suggest that actinobacteria are capable of producing metal oxide nanoparticles that can be exploited in the green synthesis of nanomaterials and utilized in biological systems. Overall, the multifunctionality of actinobacteria makes this group of microorganisms very unique, and their potentials are yet to be exploited. This book chapter highlights the

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Actinobacteria in Agricultural and Environmental Sustainability

Shivlata

Satyanarayana

2017

Agro-Environmental Sustainability

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Identification by Suppression Subtractive Hybridization of Frankia Genes Induced under Nitrogen-Fixing Conditions

Cited by 8 publications

References 22 publications

Transcriptome Analysis of Paraburkholderia phymatum under Nitrogen Starvation and during Symbiosis with Phaseolus Vulgaris

Transcriptome Analysis of Paraburkholderia phymatum under Nitrogen Starvation and during Symbiosis with Phaseolus Vulgaris

Role of Actinomycete-Mediated Nanosystem in Agriculture

Actinobacteria in Agricultural and Environmental Sustainability

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