Identification, characterization, and probiotic potential of Lactobacillus rhamnosus isolated from human milk

Rajoka, Muhammad Shahid Riaz; Mehwish, Hafiza Mahreen; Siddiq, Muhammad; Zhao, Hang; Zhu, Jing; Li, Yan; Shao, Dongyan; Xu, Xiaoguang; Shi, Junling

doi:10.1016/j.lwt.2017.05.055

Cited by 159 publications

(92 citation statements)

References 51 publications

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“…All of the tested four Lactobacillus strains were found to be resistant to streptomycin and kanamycin but sensitive towards tetracycline, ampicillin and erythromycin ( Table). These results were found in agreement with the results that were obtained using Lactobacillus species 22 (32). Tigu, Assefa (14) have also reported that all of the LAB isolates obtained from traditional fermented condiments such as Datta and Awaze were susceptible to ampicillin, erythromycin and tetracycline.…”

Section: Discussionsupporting

confidence: 89%

In vitro evaluation of probiotic properties of lactic acid bacteria isolated from some traditionally fermented Ethiopian food products

Mulaw

Sisay

Muleta

et al. 2019

Preprint

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Probiotics are live microorganisms which when consumed in large number together with a food promote the health of the consumer. The aim of this study was to evaluate in vitro probiotic properties of lactic acid bacteria (LAB) isolated from traditional Ethiopian fermented Teff injera dough, Ergo and Kocho products. A total of 90 LAB were isolated, of which 4 (4.44%) isolates showed 45.35-97.11% and 38.40-90.49% survival rate at pH values (2, 2.5 and 3) for 3 and 6 h in that order. The four acid tolerant isolates were found tolerant to 0.3% bile salt for 24 h with 91.37 to 97.22% rate of survival. The acid-and-bile salt tolerant LAB isolates were found inhibiting some foodborne test pathogenic bacteria to varying degrees. All acid-and-bile tolerant isolates displayed varying sensitivity to different antibiotics. The in vitro adherence to stainless steel plates of the 4 screened probiotic LAB isolates were ranged from 32.75 to 36.30% adhesion rate. The four efficient probiotic LAB isolates that belonged to Lactobacillus species were identified to strain level using 16S rDNA gene sequence comparisons and namely were Lactobacillus plantarum strain CIP 103151, Lactobacillus paracasei subsp. tolerans strain NBRC 15906, Lactobacillus paracasei strain NBRC 15889 and Lactobacillus plantarum strain JCM 1149. The four Lactobacillus strains were found to have potentially useful to produce probiotic products.

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Section: Discussionsupporting

confidence: 89%

In vitro evaluation of probiotic properties of lactic acid bacteria isolated from some traditionally fermented Ethiopian food products

Mulaw

Sisay

Muleta

et al. 2019

Preprint

View full text Add to dashboard Cite

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“…[7,171,187,188]. Among the genera isolated in milk, many species belonging to Bifidobacterium and Lactobacillus have been isolated, such as Bifidobacterium breve, Bifidobacterium bifidum, Bifidobacterium adolescentis, Lactobacillus gasseri, Lactobacillus fermentum, Lactobacillus plantarum, Lactobacillus rhamnosus, and Lactobacillus salivarius [9,11,[189][190][191][192][193].…”

Section: Breast Milk Microbiomementioning

confidence: 99%

“…The human milk microbiota has both immediate and long-term roles in reducing and preventing the incidence and severity of bacterial infections in breastfed infants by multiple mechanisms [194]. Such mechanisms include the production of antimicrobial compounds against pathogenic bacteria, competitive exclusion, prevention of adhesion of pathogenic bacteria to intestinal epithelium, and enhancing intestinal mucin production [9][10][11]244,245]. It has been documented that potentially probiotic species of Lactobacillus have been reported to prevent intestinal adhesion of pathogenic bacteria such as Shigella spp., Salmonella spp., and Escherichia coli [9,10,245,246].…”

Section: Benefits Of Breast Feedingmentioning

confidence: 99%

“…Breast milk plays a vital role in inoculating the infant gut with bacteria after birth. Acknowledgement of the human milk microbiome as an asset to infant health has resulted in numerous investigations to elucidate its mechanisms of action, which include production of antimicrobial compounds, preventing adhesion of pathogenic bacteria to intestinal epithelium and enhancing intestinal mucin production [9][10][11]. Furthermore, the origin of bacteria in breast milk has led to much debate, with multiple studies investigating potential pathways that give rise to bacteria in milk such as retrograde backflow and the possible existence of an entero-mammary pathway [12].…”

Section: Introductionmentioning

confidence: 99%

“…As the health promoting benefits of bacteria in breast milk have been established, the isolation of potential probiotic strains from milk has been the focus of some investigations [9,11]. The isolation of potential probiotic strains has been limited to traditional bacterial species of Bifidobacterium and Lactobacillus due to a long track record of safety and efficacy in nutrition and health markets.…”

Section: Introductionmentioning

confidence: 99%

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Breast Milk, a Source of Beneficial Microbes and Associated Benefits for Infant Health

et al. 2020

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Human breast milk is considered the optimum feeding regime for newborn infants due to its ability to provide complete nutrition and many bioactive health factors. Breast feeding is associated with improved infant health and immune development, less incidences of gastrointestinal disease and lower mortality rates than formula fed infants. As well as providing fundamental nutrients to the growing infant, breast milk is a source of commensal bacteria which further enhance infant health by preventing pathogen adhesion and promoting gut colonisation of beneficial microbes. While breast milk was initially considered a sterile fluid and microbes isolated were considered contaminants, it is now widely accepted that breast milk is home to its own unique microbiome. The origins of bacteria in breast milk have been subject to much debate, however, the possibility of an entero-mammary pathway allowing for transfer of microbes from maternal gut to the mammary gland is one potential pathway. Human milk derived strains can be regarded as potential probiotics; therefore, many studies have focused on isolating strains from milk for subsequent use in infant health and nutrition markets. This review aims to discuss mammary gland development in preparation for lactation as well as explore the microbial composition and origins of the human milk microbiota with a focus on probiotic development.

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Lactic Acid Bacteria as Potential Probiotics

Sadiq

2022

Probiotics, Prebiotics and Synbiotics

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Identification, characterization, and probiotic potential of Lactobacillus rhamnosus isolated from human milk

Cited by 159 publications

References 51 publications

In vitro evaluation of probiotic properties of lactic acid bacteria isolated from some traditionally fermented Ethiopian food products

In vitro evaluation of probiotic properties of lactic acid bacteria isolated from some traditionally fermented Ethiopian food products

Breast Milk, a Source of Beneficial Microbes and Associated Benefits for Infant Health

Lactic Acid Bacteria as Potential Probiotics

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