Identification, characterization and quantification of chemical compounds in selected edible wild leafy vegetables

Salisu, T.F.; Okpuzor, Joy; Jaja, S.I.

doi:10.4314/ijs.v21i1.19

Cited by 10 publications

(7 citation statements)

References 25 publications

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“…These include n-Hexadecanoic acid, Hexadecanoic methyl ester (palmitic acid) and α-Linolenic acid (ALA) with reported hypocholesterolemic and other lipidlowering properties. Salisu et al (2019) similarly reported high quality match palmitic, oleic and linoleic acids as Ayodele et al 3 major components out of eight compounds identified from GC-MS analysis of n-hexane extract of C. crepidioides leaf. However, this present study identified 64 compounds of phytochemical importance in C. crepidioides leaf hexane fraction obtained from the methanol (crude) extract.…”

Section: Discussionmentioning

confidence: 87%

Phytochemical Profiling of the Hexane fraction of Crassocephalum crepidioides Benth S. Moore leaves by GC-MS

Ayodele¹,

Onajobi²,

Osoniyi³

2020

Afr. J. Pure Appl. Chem.

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Crassocephalum crepidioides is an edible plant which is also used in the ethnomedical treatment of stomach ulcer, indigestion, wounds, boils and burns in Africa and some other parts of the world. This study aims at identifying and characterizing the bioactive compounds present in C. crepidioides hexane fraction which may be responsible for the ethnomedicinal uses and reported activities of the plant. The crude extract from the powdered leaves of C. crepidioides was obtained with 70% methanol, followed by solvent partitioning with hexane to give the hexane fraction which was subjected to phytochemical profiling using gas chromatography-mass spectrometry (GC-MS). Spectrum interpretation was obtained from the library search of the database of National Institute of Standards and Technology (NIST), while biological activities of compounds identified were predicted based on Dr. Duke's Phytochemical and Ethnobotanical Databases. The results revealed the presence of several bioactive compounds with various biological activities including Hexadecanoic methyl ester and α-Linolenic acid with reported hypocholesterolemic properties; Benzofuranone and Benzofuran with anticancer and antiviral activities; phenolic compounds and flavonoids with reported antioxidant, anti-inflammatory and antifungal activities among others. The study showed that C. crepidioides contains compounds with important biological activities which provide scientific support for some medicinal uses of the plant.

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Section: Discussionmentioning

confidence: 87%

Phytochemical Profiling of the Hexane fraction of Crassocephalum crepidioides Benth S. Moore leaves by GC-MS

Ayodele¹,

Onajobi²,

Osoniyi³

2020

Afr. J. Pure Appl. Chem.

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“…Vitamin E or tocopherol and its various biological forms including gamma and delta tocopherol are predominantly known for their antioxidant properties. Phytol is a terpenoid used as a precursor for synthesis of fat soluble vitamins E and K1 [53] with strong antioxidant and possess anti-inflammatory activity [54]. Lup-20(29)-en-3-ol, acetate, (3β)-, a triterpenoid also known as lupeol acetate possess antimicrobial, antiinflammatory, antimalarial, antituberculosis, antinociceptive and anticancer activity against breast cancer cells MCF-7 [55,56].…”

Section: Resultsmentioning

confidence: 99%

GC-MS Analysis, Antiglycation, Antioxidant and Anti-lipid Peroxidation Activities of Harungana madagascariensis Methanolic Stem Bark Extract

Adetayo,

Modupe,

Sunday

et al. 2023

Trends Sci

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Plants’ natural products make excellent lead for the development of new drugs used for prevention, management and treatment of communicable and non-communicable diseases. The specific aim of the study was to investigate the antioxidant and antiglycation activities of Harungana madagascariensis methanolic stem bark extract. The active compounds present in the plant were extracted using methanol by maceration and resulting extract was subjected to phytochemical screening, antioxidant and antiglycation analysis. The extract was fractioned on silica gel to isolate the antioxidant compounds domiciled in the extract and fractions were identified by bioautography. The H. madagascariensis methanolic stem bark extract showed the presence of tannins, alkaloids, saponins, steroids and flavonoids. From the antioxidant assays, the extract scavenged 2,2-diphenyl-1-picryhydrazyl (DPPH) and nitric oxide radicals, inhibited lipid peroxidation in a dose dependent manner showing IC50 higher than ascorbic acid while it inhibited albumin glycation with IC50 lower than ascorbic acid. From the 12 fractions obtained only 7 fractions showed antioxidant activity and quercetin, flavone, 6-hydroxyflavone, ferulic and o-coumaric acid were inferred. From the GC-MS analysis, fatty acids and their esters, terpenoids and steroids were identified such as Hexadecanoic acid ethyl ester (6.53 %), 9,12,15-Octadecatrienoic acid (5.92 %), 9,12,15-Octadecatrienoic acid methyl ester (0.95 %), vitamin E (1.22 %), phytol (3.87 %), squalene (1.66 %) and Pregn-5-en-3-ol, 21-bromo-20-methyl-(3.beta.)- (1.94 %). In conclusion, H. madagascariensis methanolic stem bark extract possessed antioxidant and antiglycation activities, thus, can be used for new drug development to reduce or combat oxidative stress related diseases in humans. HIGHLIGHTS The methanolic stem bark extract of Harungana madagascariensis exhibited strong antiglycation and antioxidant activities Antioxidant compounds including flavonoids and phenolic acids (quercitin, flavone, 6-hydroxyflavone, ferulic and o-coumaric acid), terpenoids (vitamin E, phytol and squalene) fatty acids and their esters were found in the extract The stem bark extract of Harungana madagascariensis could be used as a source of antiglycation and antioxidant activity for new drug development in preventing oxidative stress related diseases and diabetic complications GRAPHICAL ABSTRACT

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“…In a study, polyunsaturated fatty acids were the main group of fatty acids documented in all the species of coral fungi from Northwestern Himalayas (Sharma & Guatam 2017). These bioactive compounds are known to have potent antimicrobial, antioxidant, anti-inflammatory and antistaphylococcal properties (Ogidi et al 2015;Falade et al 2017;Salisu et al 2019;Akingbesote & Oyetayo 2021). Ogidi (2017) had earlier reported the presence of these groups of fatty acids in bioactive extracts obtained from Lenzites quercina.…”

Section: Discussionmentioning

confidence: 99%

Assessment of the antistaphylococcal properties and bioactive compounds of raw and fermented Trametes polyzona (Pers.) Justo extracts

Oyetayo

Akingbesote

2022

MBJ

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Trametes species are polyporoid white rot fungi that are important in medicine as well as serving as bioremediator and biodegrader of cellulosic waste. The present study investigates the bioactive compounds and antistaphylococcal properties of extracts obtained from raw and fermented Trametes polyzona. T. polyzona was collected from dead woods in farmland around Akungba-Akoko (7 o 27' 32.4''N 5 o 45' 07.8''E) and subjected to submerged and solid-state fermentation. The raw and fermented samples were extracted using acetone and methanol. The mycochemical and antistaphylococcal properties of the extract obtained from raw and fermented T. polyzona were assessed using standard methods. Extract obtained from T. polyzona subjected to submerged fermentation exhibited higher and better antistaphylococcal activity (28.00 mm) on Staphylococcus aureus isolated from blood when compared to other extracts. The minimum inhibitory concentration of the extracts against Staphylococcus aureus ranged from 100mg/mL to 400mg/mL. Gas chromatography Mass Spectrophometric (GC-MS) analysis of the sample confirmed the presence of fourteen bioactive compounds of fatty acid group. Results from this study revealed that extracts obtained from T. polyzona contain bioactive compounds with potent antistaphylococcal properties that can be exploited by biopharmaceutical companies.

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Identification, characterization and quantification of chemical compounds in selected edible wild leafy vegetables

Cited by 10 publications

References 25 publications

Phytochemical Profiling of the Hexane fraction of Crassocephalum crepidioides Benth S. Moore leaves by GC-MS

Phytochemical Profiling of the Hexane fraction of Crassocephalum crepidioides Benth S. Moore leaves by GC-MS

GC-MS Analysis, Antiglycation, Antioxidant and Anti-lipid Peroxidation Activities of Harungana madagascariensis Methanolic Stem Bark Extract

Assessment of the antistaphylococcal properties and bioactive compounds of raw and fermented Trametes polyzona (Pers.) Justo extracts

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