Identification, genetic diversity and prevalence of Theileria and Babesia species in a sheep population from Northern Spain

Nagore, Daniel; Garcı́a-Sanmartı́n, Josune; Garcı́a-Pérez, Ana L.; Juste, Ramón A.; Hurtado, Ana

doi:10.1016/j.ijpara.2004.05.008

Cited by 139 publications

(108 citation statements)

References 29 publications

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“…Therefore, development of a highly specific and sensitive method for the diagnosis of theileriosis infections is required. Recently, molecular techniques have become the preferred methods for diagnosis of babesiosis and theileriosis, because these techniques are more sensitive and specific than other conventional methods (Alhassan et al 2005, Altay et al 2005, Nagore et al 2004.…”

Section: Discussionmentioning

confidence: 99%

Detection of Theileria annulata in blood samples of native cattle by PCR and smear method in Southeast of Iran

2013

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Theileria annulata, a protozoan parasite of cattle is causes tropical theileriosis. Polymerase chain reaction (PCR) was used to assess the presence and the frequency of T. annulata infection in blood samples obtained from carrier cattle in Kerman, Southeast of Iran. Blood samples were collected in citrate solution from 150 native cattle with mean age of 1 year which selected randomly. Primarily, a thin layer smear was prepared from their ear sublime vein blood and was fixed with methanol and stained with Giemsa dye. Blood smears were examined for the presence of parasites, and blood samples were analyzed by PCR. Piroplasmic forms of T. annulata were seen in 16 of 150 (10.66 %) by examination the blood smears with light microscope, whereas 68 of 150 (45.33 %) cattle were positive by PCR method. All animals that were positive by blood smears were also positive by PCR. Difference between these methods was significant (P \ 0.05). Our results demonstrate that this PCR assay in diagnosing T. annulata parasites in carrier cattle is more sensitive than method of smear preparation and can be used in epidemiological studies.

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Section: Discussionmentioning

confidence: 99%

Detection of Theileria annulata in blood samples of native cattle by PCR and smear method in Southeast of Iran

2013

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“…This assay enables the identification of mixed infections and has also proven to be a valuable tool in the identification of novel piroplasm species or genotypes (Georges et al, 2001, Criado-Fornelio et al, 2004, Nagore et al, 2004a, Nagore et al, 2004band Nijhof et al, 2005.…”

mentioning

confidence: 99%

Sequence heterogeneity in the 18S rRNA gene within Theileria equi and Babesia caballi from horses in South Africa

Bhoora

Franssen

Oosthuizen

et al. 2009

Veterinary Parasitology

131

126

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“…The most commonly used tests are ELISA, PCR and a DNA probe, which can detect specific parasitemias at very low levels of infection (Radostits, 2008). Recently, the 'reverse line blot (RLB) is a versatile technique for simultaneous detection and identification of small ruminant piroplasm species, based on the recognition of specific gene regions by oligonucleotide probes (Nagore et al, 2004;Qingli et al, 2009;Inci et al, 2010;). …”

Section: Diagnosismentioning

confidence: 99%

Protozoan Diseases in Farm Ruminants

Şahinduran¹

2012

A Bird's-Eye View of Veterinary Medicine

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Identification, genetic diversity and prevalence of Theileria and Babesia species in a sheep population from Northern Spain

Cited by 139 publications

References 29 publications

Detection of Theileria annulata in blood samples of native cattle by PCR and smear method in Southeast of Iran

Detection of Theileria annulata in blood samples of native cattle by PCR and smear method in Southeast of Iran

Sequence heterogeneity in the 18S rRNA gene within Theileria equi and Babesia caballi from horses in South Africa

Protozoan Diseases in Farm Ruminants

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