Identification in milk of a serum amyloid A peptide chemoattractant for B lymphoblasts

Rodríguez, Berardo de J; Chevaleyre, Claire; Henry, Gwénaële; Mollé, Daniel; Virlogeux-Payant, Isabelle; Berri, Mustapha; Boulay, François; Léonil, Joëlle; Meurens, François; Salmon, Henri

doi:10.1186/1471-2172-10-4

Cited by 16 publications

(10 citation statements)

References 60 publications

(67 reference statements)

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“…Our observation of SAA3 being exclusive to porcine colostrum is well in line with reports of bovine, equine, and ovine mammary-associated isoforms found in colostrum from healthy animals [49]. Also, previous studies of SAA in pigs have suggested the expression of a mammary-specific SAA isoform [57,58]. Our observations across the pig Pep-tideAtlas clearly suggest a local secretion of the SAA3 isoform to the colostrum and not found in other tissues.…”

Section: Tissue-enriched Expression Of Saa Isoformssupporting

confidence: 91%

“…This observation is interesting in the light that the pig mammary gland related SAA isoform has been observed to increase during pregnancy, peaking at day 15 of lactation . Also, a specific peptide from SAA3 (DMREANYKNSDKYFHARGNYDAA) was found to act as a chemoattractant for immune cells , indicating that the mammary‐associated isoform of SAA3 may have an important biological role in host response against pathogens, both in the mammary gland and in the gut of the young piglet upon uptake of milk and colostrum proteins.…”

Section: Resultsmentioning

confidence: 87%

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The Pig PeptideAtlas: A resource for systems biology in animal production and biomedicine

et al. 2016

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Biological research of Sus scrofa, the domestic pig, is of immediate relevance for food production sciences, and for developing pig as a model organism for human biomedical research. Publicly available data repositories play a fundamental role for all biological sciences, and protein data repositories are in particular essential for the successful development of new proteomic methods. Cumulative proteome data repositories, including the PeptideAtlas, provide the means for targeted proteomics, system wide observations, and cross species observational studies, but pigs have so far been underrepresented in existing repositories. We here present a significantly improved build of the Pig PeptideAtlas, which includes pig proteome data from 25 tissues and three body fluid types mapped to 7139 canonical proteins. The content of the Pig PeptideAtlas reflects actively ongoing research within the veterinary proteomics domain, and this manuscript demonstrates how the expression of isoform-unique peptides can be observed across distinct tissues and body fluids. The Pig PeptideAtlas is a unique resource for use in animal proteome research, particularly biomarker discovery and for preliminary design of SRM assays, which are equally important for progress in research that supports farm animal production and veterinary health, as for developing pig models with relevance to human health research.

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Section: Tissue-enriched Expression Of Saa Isoformssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 87%

The Pig PeptideAtlas: A resource for systems biology in animal production and biomedicine

et al. 2016

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“…High levels of SAA have been related to states of inflammation in the mammary gland 37 . However, studies in pigs in healthy lactating mammary glands showed an increase of this gene’s mRNA in the second half of lactation, providing evidence that this peptide could play a physiological role in the normal mammary gland, stimulating neonatal gut immune responses 38 .…”

Section: Resultsmentioning

confidence: 99%

Characterization and Comparative Analysis of the Milk Transcriptome in Two Dairy Sheep Breeds using RNA Sequencing

Suárez-Vega

Klopp²,

Robert-Granié

et al. 2015

Sci Rep

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This study presents a dynamic characterization of the sheep milk transcriptome aiming at achieving a better understanding of the sheep lactating mammary gland. Transcriptome sequencing (RNA-seq) was performed on total RNA extracted from milk somatic cells from ewes on days 10, 50, 120 and 150 after lambing. The experiment was performed in Spanish Churra and Assaf breeds, which differ in their milk production traits. Nearly 67% of the annotated genes in the reference genome (Oar_v3.1) were expressed in ovine milk somatic cells. For the two breeds and across the four lactation stages studied, the most highly expressed genes encoded caseins and whey proteins. We detected 573 differentially expressed genes (DEGs) across lactation points, with the largest differences being found, between day 10 and day 150. Upregulated GO terms at late lactation stages were linked mainly to developmental processes linked to extracellular matrix remodeling. A total of 256 annotated DEGs were detected in the Assaf and Churra comparison. Some genes selectively upregulated in the Churra breed grouped under the endopeptidase and channel activity GO terms. These genes could be related to the higher cheese yield of this breed. Overall, this study provides the first integrated overview on sheep milk gene expression.

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“…There was an abrupt upregulation of SAA2 (serum amyloid A-2 protein) [FC = 3039.23] at 1d postpartum. This isoform is considered the most predominant member of the SAA family expressed in the swine mammary gland [ 70 ]. Rodriguez et al [ 70 ] reported that SAA mRNA production in swine increased during lactation and stimulates the neonatal immune response by enhancing the recruitment of mucosal gut B lymphoblasts and potentially influencing Ig concentrations.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional profiling of swine mammary gland during the transition from colostrogenesis to lactogenesis using RNA sequencing

et al. 2018

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BackgroundColostrum and milk are essential sources of antibodies and nutrients for the neonate, playing a key role in their survival and growth. Slight abnormalities in the timing of colostrogenesis/lactogenesis potentially threaten piglet survival. To further delineate the genes and transcription regulators implicated in the control of the transition from colostrogenesis to lactogenesis, we applied RNA-seq analysis of swine mammary gland tissue from late-gestation to farrowing. Three 2nd parity sows were used for mammary tissue biopsies on days 14, 10, 6 and 2 before (−) parturition and on day 1 after (+) parturition. A total of 15 mRNA libraries were sequenced on a HiSeq2500 (Illumina Inc.). The Dynamic Impact Approach and the Ingenuity Pathway Analysis were used for pathway analysis and gene network analysis, respectively.ResultsA large number of differentially expressed genes were detected very close to parturition (−2d) and at farrowing (+ 1d). The results reflect the extraordinary metabolic changes in the swine mammary gland once it enters into the crucial phases of lactogenesis and underscore a strong transcriptional component in the control of colostrogenesis. There was marked upregulation of genes involved in synthesis of colostrum and main milk components (i.e. proteins, fat, lactose and antimicrobial factors) with a pivotal role of CSN1S2, LALBA, WAP, SAA2, and BTN1A1. The sustained activation of transcription regulators such as SREBP1 and XBP1 suggested they help coordinate these adaptations.ConclusionsOverall, the precise timing for the transition from colostrogenesis to lactogenesis in swine mammary gland remains uncharacterized. However, our transcriptomic data support the hypothesis that the transition occurs before parturition. This is likely attributable to upregulation of a wide array of genes including those involved in ‘Protein and Carbohydrate Metabolism’, ‘Immune System’, ‘Lipid Metabolism’, ‘PPAR signaling pathway’ and ‘Prolactin signaling pathway’ along with the activation of transcription regulators controlling lipid synthesis and endoplasmic reticulum biogenesis and stress response.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4719-5) contains supplementary material, which is available to authorized users.

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Identification in milk of a serum amyloid A peptide chemoattractant for B lymphoblasts

Cited by 16 publications

References 60 publications

The Pig PeptideAtlas: A resource for systems biology in animal production and biomedicine

The Pig PeptideAtlas: A resource for systems biology in animal production and biomedicine

Characterization and Comparative Analysis of the Milk Transcriptome in Two Dairy Sheep Breeds using RNA Sequencing

Transcriptional profiling of swine mammary gland during the transition from colostrogenesis to lactogenesis using RNA sequencing

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