Identification, molecular evolution of toll-like receptors in a Tibetan schizothoracine fish (Gymnocypris eckloni) and their expression profiles in response to acute hypoxia

Qi, Di; Xia, Mingzhe; Yan, Chao; Zhao, Yanni; Wu, Rongrong

doi:10.1016/j.fsi.2017.07.014

Cited by 31 publications

(13 citation statements)

References 63 publications

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“…TLR5S lacks the transmembrane region and the intracellular TIR domain ( 201 ). In recent years, TLR5M and TLR5S, which are present in many tissues ( 158 , 200 , 201 ), have been identified in pufferfish ( 158 ), orange-spotted grouper ( 201 ), Atlantic salmon ( 202 ), Japanese flounder ( 203 , 204 ), catfish ( 205 ), Tibetan schizothoracine fish ( 197 , 206 ), and turbot ( 207 ). Previous studies on Atlantic salmon, Japanese flounder, pufferfish, and catfish revealed that TLR5M contains 9–12 LRR domains, and that the TIR domain contains 139–151 amino acids.…”

Section: Tlrs In Non-mammalian Vertebratesmentioning

confidence: 99%

“…The TLR7 subfamily contains TLR7, TLR8, and TLR9, which are structurally similar to the mammalian homologs and have 13–15 LRR domains ( 160 , 194 , 211 – 214 ). In recent years, TLR7–9 have been identified in channel catfish ( 152 ), Atlantic cod ( 178 ), Tibetan schizothoracine fish ( 206 ), common carp ( 211 ), zebrafish ( 160 ), rainbow trout ( 215 ), grass carp ( 216 , 217 ), Atlantic salmon ( 218 , 219 ), yellow catfish ( 220 ), turbot ( 221 ), flounder ( 222 ), and sea bream ( 223 ). Kileng and colleagues found that the imiquimod derivative S-27609 induces the upregulation of IFN-α1/α2 and IFN-γ expression in the liver and head kidney tissues in Atlantic salmon through a TLR7-like receptor, providing indirect evidence for the presence of TLR7 signaling pathways in fish ( 214 ).…”

Section: Tlrs In Non-mammalian Vertebratesmentioning

confidence: 99%

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Toll-Like Receptors, Associated Biological Roles, and Signaling Networks in Non-Mammals

et al. 2018

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The innate immune system is the first line of defense against pathogens, which is initiated by the recognition of pathogen-associated molecular patterns (PAMPs) and endogenous damage-associated molecular patterns (DAMPs) by pattern recognition receptors (PRRs). Among all the PRRs identified, the toll-like receptors (TLRs) are the most ancient class, with the most extensive spectrum of pathogen recognition. Since the first discovery of Toll in Drosophila melanogaster, numerous TLRs have been identified across a wide range of invertebrate and vertebrate species. It seems that TLRs, the signaling pathways that they initiate, or related adaptor proteins are essentially conserved in a wide variety of organisms, from Porifera to mammals. Molecular structure analysis indicates that most TLR homologs share similar domain patterns and that some vital participants of TLR signaling co-evolved with TLRs themselves. However, functional specification and emergence of new signaling pathways, as well as adaptors, did occur during evolution. In addition, ambiguities and gaps in knowledge still exist regarding the TLR network, especially in lower organisms. Hence, a systematic review from the comparative angle regarding this tremendous signaling system and the scenario of evolutionary pattern across Animalia is needed. In the current review, we present overview and possible evolutionary patterns of TLRs in non-mammals, hoping that this will provide clues for further investigations in this field.

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Section: Tlrs In Non-mammalian Vertebratesmentioning

confidence: 99%

Section: Tlrs In Non-mammalian Vertebratesmentioning

confidence: 99%

Toll-Like Receptors, Associated Biological Roles, and Signaling Networks in Non-Mammals

et al. 2018

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“…After sampling for feeding trial, the remaining fishes in each tank were exposed to a hypoxia condition according to the method previously outlined (Qi, Xia, Chao, Zhao, & Wu, 2017). The DO was controlled at 0.5 ± 0.1 mg/L through injection of nitrogen gas into the water.…”

Section: Acute Hypoxia Stressmentioning

confidence: 99%

Dietary nano‐selenium enhances antioxidant capacity and hypoxia tolerance of grass carp Ctenopharyngodon idella fed with high‐fat diet

Cheng

Zhang

et al. 2019

Aquacult Nutr

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To evaluate the effects of dietary nano‐selenium (Nano‐Se) on antioxidant capacity and hypoxia tolerance of grass carp fed with high‐fat diet, experimental fishes were fed Nano‐Se supplemented diets at doses of 0 (Control), 0.3, 0.6, 0.9 and 1.2 mg/kg for 10 weeks. After feeding trial, a part of the fishes were exposed to hypoxia stress. Results showed that the survival ratio of grass carp significantly increased in 0.6 and 0.9 mg/kg Nano‐Se group, and the content of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) significantly decreased in 0.6–1.2 mg/kg Nano‐Se groups compared with the control group. In addition, dietary Nano‐Se significantly enhanced glutathione peroxidase (GPX) activity and reduced the malondialdehyde (MDA) content in fishes fed diets with 0.3 and 0.6 mg/kg Nano‐Se. Dietary Nano‐Se significantly elevated mRNA expression of GPX1 and catalase (CAT) by promoting the mRNA expression of NF‐E2‐related nuclear factor 2 (Nrf2) in the hepatopancreas. After hypoxia stress, the GPX and superoxide dismutase (SOD) activities were significantly enhanced, and the MDA content and mortality rate consequently decreased in fishes fed diets with 0.3 and 0.6 mg/kg Nano‐Se. In summary, these results suggested that optimal Nano‐Se in diet enhanced the antioxidant capacity and hypoxia tolerance of grass carp.

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“…Notably, a growing body of studies provided the evidences of immune genes showing signatures of rapid evolution and positive selection in Schizothoracine fishes, including Gymnocypris przewalskii [16,23,24] and Gymnocypris eckloni [25][26][27]. Among Schizothoracine fishes, Tibetan naked carp (Gymnocypris przewalskii) is one of the best characterized Tibetan endemic fish species, and a number of key genes contributed to its highland adaptation were identified by previous studies [7,13,14,16,24,28].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic signature of rapidly evolving immune genes in a highland fish

Tong

2020

Fish & Shellfish Immunology

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Recent genome-wide studies have begun to elucidate the genomic basis of hypoxia, long-term cold and high saline and alkaline adaptation in highland fish, and a number of key genes contributed to its highland adaptation were identified. An increasing number of studies indicated that immune genes of Tibetan endemic fish species underwent positive selection towards functional shift, while the insight into immune gene repertoire of Tibetan highland fishes from genome-wide studies has largely lagged behind. In this study, we performed one of the first comparative genomics study in particular focusing on the signatures of immune genes in a highland fish, Gymnocypris przewalskii based on immune-relevant tissue transcriptome assemblies. We identified seven putative rapidly evolving immune genes with elevated molecular evolutionary rate (dN/dS) relative to lowland fish species. Using tissue-transcriptome data, we found most of rapidly evolving immune genes were broadly expressed in head-kidney, spleen, gills and skin tissues, which significantly enriched for complement activation and inflammatory response processes. In addition, we found a set of complement activation related genes underwent accelerated evolution and showed consistently repressed expression patterns in response to parasite Ichthyophthirius multifiliis infection. Moreover, we detected a number of immune genes involved in adaptive immune system exhibited distinct signature of upregulated expression patterns after parasite infection. Taken together, this study provided putative transcriptomic signatures of rapidly evolving immune genes, and will gain the insight into Schizothoracine fish adaptation to high-altitude extreme aquatic environments including diversified pathogen challenge.

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Identification, molecular evolution of toll-like receptors in a Tibetan schizothoracine fish (Gymnocypris eckloni) and their expression profiles in response to acute hypoxia

Cited by 31 publications

References 63 publications

Toll-Like Receptors, Associated Biological Roles, and Signaling Networks in Non-Mammals

Toll-Like Receptors, Associated Biological Roles, and Signaling Networks in Non-Mammals

Dietary nano‐selenium enhances antioxidant capacity and hypoxia tolerance of grass carp Ctenopharyngodon idella fed with high‐fat diet

Transcriptomic signature of rapidly evolving immune genes in a highland fish

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