Identification of 2-hydroxyoestradiol and the pattern of catechol oestrogens in human pregnancy urine

Gelbke, H; Hoogen, H.; Knüppen, R.

doi:10.1016/0022-4731(75)90101-6

Cited by 20 publications

(2 citation statements)

References 15 publications

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“…Considerably lower cross-reacti¬ vities were found with 2-methoxyoestriol, 2-methoxyoestradiol and oestriol. Considering this pat¬ tern of cross-reactivity and expecting a low serum concentration of 2-hydroxyoestriol (Gelbke et al 1975), we concluded that the 125I-radioligand RIA system is quite suitable for the quantitative deter¬ mination of total 2-hydroxyoestrogens in serum with high specificity and sensitivity. The concentration of 2-hydroxyoestrogens found in various physiological situations are in good agreement with the values reported by Ball et al.…”

Section: Discussionmentioning

confidence: 94%

Concentrations of 2-hydroxyoestrogens in human sera measured by a heterologous immunoassay with an 125I-labelled ligand

Berg

Thaler

Kuß

1982

Acta Endocrinologica

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A heterologous immunoassay for 2-hydroxyoestrogens1 has been established in which antibodies raised against 2-hydroxyoestradiol-17-succinyl-BSA serve as binding protein and 2-hydroxyoestrone-17-cmo\x=req-\[125I]iodohistamine as radioligand. Lipophilic serum components competing for binding sites in this system were defined as 'total 2-hydroxyoestrogens'. The underlying assumption of specificity was supported by the pattern of cross-reactivity evaluated with structural related steroids and o-diphenols and by the fact, that an additional chromatography of the serum extracts preceding the competing reaction had little if any effect. Sensitivity: 2.8 \m=+-\1 pg/tube; accuracy: Y = 0.91x + 2.2; r = 0.989; precision: 5.8% intra-assay; 6.5% inter-assay. The following concentrations ( \ m=+-\ standard deviation) were found in the sera of healthy subjects.Young men: 29 \m=+-\5 pg/ml (n = 11); women follicular phase: 32 \m=+-\8 pg/ml (n = 25); luteal phase: 53 \m=+-\13 pg/ml (n = 23); postmenopausal women: 13 \m=+-\4 pg/ml (n = 10); pregnant women 11th\p=n-\20th week: 70 \m=+-\16 mg/ml (n = 64); 36th\p=n-\40thweek: 240 \m=+-\23 pg/ml (n = 40); newborn cord blood: 604 \m=+-\43 pg/ml (n = 48).2-Hydroxyoestrogens have been recognized as major metabolites of oestrone and oestradiol in both laboratory animals and humans. The opinions on their physiological significance are still at vari¬ ance (Ball & Knuppen 1980). The assay of cate¬ choloestrogens in biological fluids and tissue by the classical methods of steroid separation and detec¬ tion is hampered by their chemical instability. They are highly susceptible to attack by oxidants, especi¬ ally in alcaline media. In addition, the classical methods are time consuming, expensive and not applicable for routing determinations. These pro¬ blems have been mastered by the development of suitable radioimmunoassays (Chattoraj et al. 1978;Ball et al. 1978). Methods currently available utilise tritium labelled radioligands, which are not avail¬ able commercially. Their syntheses are lengthy and tedious with generally small yields. In establishing a sensitive and specific radioimmunoassay of serum catecholestrogens we preferred the use of 125I-labelled radioligands, which offer the advan¬ tages of higher specific activities and cost-saving counting for at least 3 months after preparation. In order to obtain a very high sensitivity of the assay we utilized a heterologous bridge system employing anti-2-hydroxyoestradiol-17ß-succinyl-BSA-serum and 2-hydroxyoestrone-17-cmo-[125I]-iodohistamine. Preliminary results have been pub¬ lished previously (Berg et al. 1981).1 The following trivial names and abbreviations were used: 2-hydroxyoestrone = 2,3-dihydroxy-l,3,5(10)-oestratrien-17-one, 2-hydroxyoestradiol = l,3,5(10)-oestratriene-2,3,17ß-triol, 2-hydroxyoestriol = l,3,5(10)-oestratriene-2,3,16a,17ß-tetrol, oestradiol-17ß = 1,3,5(10)-oestratriene-3,17ß-diol, oestrone = 3-hydroxy-1,3,5(10)-oestratrien-17-one, oestriol = l,3,5(10)-oestratriene-3-16a, 17ß-triol, 2-methoxyoestrone = 2-methoxy-3-h...

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Section: Discussionmentioning

confidence: 94%

Concentrations of 2-hydroxyoestrogens in human sera measured by a heterologous immunoassay with an 125I-labelled ligand

Berg

Thaler

Kuß

1982

Acta Endocrinologica

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“…Considerable attention has been recently focussed on the physiological significance of these catecholestrogens (Fishman 1981;MacLusky, Naftolin, Krey and Franks 1981). Gelbke, Hoogen and Knuppen (1975) reported that 2-hydroxyestriol (2-OHE 3 ) was quantitatively determined in several pregnancy urines by gas-liquid chromatography. From this viewpoint, it seemed necessary to investigate the concentration of 2-OHE 3 in human pregnancy plasma in connection with estriol (E 3 ).…”

Section: Introductionmentioning

confidence: 99%

Preparation and Antigenic Properties of 2-Hydroxyestriol-Bovine Serum Albumin Conjugate

Fujii¹,

Teranishi²,

Mizukami³

et al. 1984

Horm Metab Res

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The effect of histamine on parathyroid function was studied in two patients. 2.75 mg histamine phosphate administered intravenously over 120 minutes induced the well known clinical effects such as facial flushing, fall in blood pressure and increase in heart rate. But the serum calcium concentration, serum immunoreactive parathormone concentration, urinary excretion of cAMP and tubular phosphate reabsorption did not change significantly. It is concluded that stimulation of histamine receptors does not play an important role in the regulation of parathyroid activity.

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End-organ metabolism of oestrogens

MacLusky

Clark

Paden

et al. 1981

Mechanisms of Steroid Action

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Identification of 2-hydroxyoestradiol and the pattern of catechol oestrogens in human pregnancy urine

Cited by 20 publications

References 15 publications

Concentrations of 2-hydroxyoestrogens in human sera measured by a heterologous immunoassay with an 125I-labelled ligand

Concentrations of 2-hydroxyoestrogens in human sera measured by a heterologous immunoassay with an 125I-labelled ligand

Preparation and Antigenic Properties of 2-Hydroxyestriol-Bovine Serum Albumin Conjugate

End-organ metabolism of oestrogens

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