Identification of 2n breeding lines and 4n varieties of potato (Solanum tuberosum, ssp. tuberosum) with RFLP-fingerprints

Abstract

The possibility of genotype identification with RFLP fingerprints was examined with 20 tetraploid potato varieties and 38 diploid potato lines. By using a sensitive detection system for small restriction fragment length differences and highly variable potato sequences as probes, all genotypes (diploids and tetraploids) were distinguished by a minimum of two probe/enzyme combinations. The best single probe/enzyme combination distinguished 19 out of 20 4n varieties and 33 out of 38 2n lines. Intravarietal variability was very small compared to the intervarietal variability, and patterns obtained with different DNA sources of the same genotype were identical.

Genetic Markers and Plant Genetic Resource Management

“…(Kennedy and Thompson 1991) and Solanum tuberosum L. (Gebhardt et al 1989); for fruits, such as Actinidia deliciosa (A. Chev.)…”

Application of a set of 14 c-DNA probes from wheat to detect restriction fragment length polymorphism (RFLP) in barley

“…Still higher levels of polymorphism have been reported in lettuce (27% for tests involving cDNA probes ; Landry et al . 1987), in maize (Helentjaris et al ., 1985), in potato (analysis with four-cutter enzymes ; Gebhardt et al ., 1989a) and in rice (McCouch et al ., 1988) . Gale et al (1989) reported that they found a higher level of RFLP in barley than in wheat .…”

Heterozygosity in tetraploid potatoes revealed by rDNA polymorphism analysis of their dihaploid progenies: a contribution to chromosome assignment

“…Bonierbale et al [3] also revealed reduced recombination in an interspecific diploid Solanum cross. Dihaploid progenies have already allowed the analysis of structural complexity of the patatin gene family [24], whereas diploid and tetraploid genotypes have been used for genotype identification with RFLP (restriction fragment length polymorphism) fingerprints [10], RFLP analysis and linkage mapping [11] and phylogeny based on nuclear RFLPs [5].…”