Identification of a 33 kDa antigen associated with an adhesive and colonizing strain of Vibrio cholerae El Tor and its role in protection

Jacob, Alice; Sinha, V. B.; Sahib, Maharaj K.; Srivastava, Ranjana; Kaper, James B.; Srivastava, Bhavana

doi:10.1016/0264-410x(93)90203-a

Cited by 18 publications

(32 citation statements)

References 27 publications

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“…A cell surface antigen of similar mol. wt to that in the present study was reported by Jacob et al [34], who found it to be immunogenic and protective in the rabbit ileal loop model.…”

Section: Discussionsupporting

confidence: 89%

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Immunogenicity and protective role of an IgA reactive 31-kDa antigen of Vibrio cholerae O139

Kumar¹,

Ray²,

Singh³

et al. 2001

Journal of Medical Microbiology

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Immunoglobulin A (IgA) is important in protective immunity against infection by Vibrio cholerae. In this study, the immune response to and protective role of a 31-kDa antigen of V. cholerae O139, reacting with IgA antibodies present in the sera of cholera patients and common to V. cholerae strains O139 and O1 was evaluated in BALB/c mice. From the various antigens of V. cholerae O139 and V. cholerae O1 which reacted with IgA antibodies in sera of a cholera patient, a 31-kDa common antigen was selected and puri®ed by DEAE-Sepharose CL 6B column chromatography. Oral administration of live V. cholerae O139 in BALB/c mice elicited an IgA response to the 31-kDa antigen in serum and intestinal¯uid, and a proliferation of the splenic lymphocytes on stimulation with the same antigen. The cytokine pro®le of these splenic lymphocytes revealed a shift from a mixed Th1 and Th2 response ± interleukin-10 (IL-10) and interferon-ã ± in the ®rst week after infection to a Th2 type of response ± IL-10 ± in the third week. In passive protection studies, hyperimmune serum to the 31-kDa antigen was able to protect infant mice against challenge with O139 and O1 strains. These results demonstrate the ability of the 31-kDa antigen of V. cholerae O139 to induce humoral and cellular immune responses in mice, and its immunoprotective nature.

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“…A cell surface antigen of similar mol. wt to that in the present study was reported by Jacob et al [34], who found it to be immunogenic and protective in the rabbit ileal loop model.…”

Section: Discussionsupporting

confidence: 89%

“…Sears et al [17] reported a 31-kDa protein, as a minor OMP in V. cholerae O1, which was common to the strains of both biotypes and serotypes. An OMP of 30 kDa and a cell surface protein of 33 kDa have also been described [12,34]. Therefore, it is possible that the 31-kDa protein identi®ed in this study is an OMP.…”

Section: Discussionmentioning

confidence: 77%

Immunogenicity and protective role of an IgA reactive 31-kDa antigen of Vibrio cholerae O139

Kumar¹,

Ray²,

Singh³

et al. 2001

Journal of Medical Microbiology

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“…Adherence of V015 and TnphoA mutants to freshly isolated rabbit intestinal discs was measured as described previously [9]. The adherence index is the number of adherent vibrios expressed as a percentage of the total number of vibrios to which the disc of intact rabbit intestinal mucosa was exposed.…”

Section: Adherencementioning

confidence: 99%

“…Swiss albino rabbits weighing 1 kg were immunised subcutaneously with two injections (50 pg each dose) of 40-kDa protein and challenged with lo4 cells of V015 in the ileal loop model as described previously [9]. The 40-kDa antigen was obtained by separating the total proteins of V015 by SDS-PAGE.…”

Section: Immunisation and Protectionmentioning

confidence: 99%

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Screening of TnPhoA Mutants of Vibrio Cholerae O139 for Identification of Antigens Involved in Colonisation

Bondre

Srivastava²,

Sinha³

et al. 1997

Journal of Medical Microbiology

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A new serogroup of Vibrio cholerae non-01, designated as 0139, has emerged causing cholera-like disease among adults. Laboratory and field studies clearly show that there is no cross-protection between 01 and 0139 pathogenic strains. Since colonisation of the intestine is a most important step in the pathogenesis of cholera caused by 01 strains and colonising antigens are known to be protective, investigation of the colonising antigens of 0139 strain was initiated. By TnphoA mutagenesis, mutants were generated with insertions in the genome encoding membrane spanning or secretory proteins. Screening of the mutants for adherence to rabbit intestinal surface and colonisation in 5-day-old mice resulted in the identification of mutant clones, which were less adhesive than was the wild-type parent strain and which could not efficiently colonise the gut. Such non-colonising strains were attenuated in virulence. Analysis of the proteins by SDS-PAGE revealed that the non-colonising membrane protein.

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Cholera and Drinking Water

Srivastava¹

2013

Water and Health

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Identification of a 33 kDa antigen associated with an adhesive and colonizing strain of Vibrio cholerae El Tor and its role in protection

Cited by 18 publications

References 27 publications

Immunogenicity and protective role of an IgA reactive 31-kDa antigen of Vibrio cholerae O139

Immunogenicity and protective role of an IgA reactive 31-kDa antigen of Vibrio cholerae O139

Screening of TnPhoA Mutants of Vibrio Cholerae O139 for Identification of Antigens Involved in Colonisation

Cholera and Drinking Water

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