Identification of a 66kDa Haemonchus contortus excretory/secretory antigen that inhibits host monocytes

Rathore, Dharmendar; Suchitra, S.; Saini, Mohini; Singh, Bhupinder; Joshi, Pallavi

doi:10.1016/j.vetpar.2006.01.055

Cited by 34 publications

(33 citation statements)

References 21 publications

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“…The survival in the host reflects the ability of parasites to evade the host immune responses from the early stages of infection [1, 2]. Excretory and secretory products (ESPs) are produced and released by the parasites during in vitro cultivation [3] and in vivo [4]. ESPs contain various proteins and are capable to induce the depression or instigation of the host immune response and are related to the pathogenesis of the parasites [5–7].…”

Section: Introductionmentioning

confidence: 99%

“…ESPs contain various proteins and are capable to induce the depression or instigation of the host immune response and are related to the pathogenesis of the parasites [5–7]. Binding to the host cell is a prerequisite for ESP function [3, 8–11]. Some ESP molecules react to the molecules on the surface of the host cell to form receptor-ligand complexes, similar to many other receptor-ligand systems.…”

Section: Introductionmentioning

confidence: 99%

“…A 55kDa secretory glycoprotein was identified with the ability to inhibit host neutrophils [8]. The purified 66-kDa adult H. contortus excretory/secretory (E/S) antigen was confirmed to suppress monocyte function in vitro by decreasing the production of hydrogen peroxide and nitric oxide in the culture medium of the cells [3]. Recently, it was reported that recombinant H. contortus galectin (rHco-gal-m) inhibited the expression of MHC II molecules, decreased the T cell activation and proliferation, induced apoptosis of T cells and effected several signaling cascades in vitro [10].…”

Section: Introductionmentioning

confidence: 99%

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Haemonchus contortusexcretory and secretory proteins (HcESPs) suppress functions of goat PBMCsin vitro

Gadahi

Ehsan

et al. 2016

Oncotarget

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Excretory and secretory products (ESPs) of nematode contain various proteins which are capable of inducing the instigation or depression of the host immune response and are involved in the pathogenesis of the worms. In the present study, Haemonchus contortus excretory and secretory products (HcESPs) were collected from the adult worms. Binding of HcESPs to goat peripheral blood mononuclear cells (PBMCs) was confirmed by immune-fluorescence assay. Effects of the HcESPs on cytokine production, cell proliferation, cell migration and nitric oxide (NO) production of PBMCs were checked by co-incubation of HcESPs with goat PBMCs. The results indicated that the production of IL-4 and IFN-γ were significantly decreased by HcESPs in dose dependent manner. On the contrary, the production of IL-10 and IL-17 were increased. Cell migration was significantly enhanced by HcESPs, whereas, HcESPs treatment significantly suppressed the cell proliferation and NO production. These results indicated that the HcESPs played important suppressive regulatory roles on PBMCs and provided highlights to the understanding of the host-parasite interactions.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Haemonchus contortusexcretory and secretory proteins (HcESPs) suppress functions of goat PBMCsin vitro

Gadahi

Ehsan

et al. 2016

Oncotarget

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“…3). Ashman et al (1995) purified a 70-90 kDa larval protein from H. contortus ;Fetterer et al (1999) purified protein from adult H. contortus and its molecular weight was determined as 33 kDa by SDS-PAGE; Gomez-Munoz et al (1996) identified and purified a 26 kDa protein partially from H. contortus by SDS-PAGE; Crab et al (2002) partially purified 23.8 kDa protein from this parasite; Rathore et al (2006) identified 66 kDa excretory secretory antigen from H. contortus; Prasad et al (2007) recognized 60 and 120 kDa polypeptides from H. contortus by immunoaffinity chromatography and SDS-PAGE; Selvarayar Arunkumar (2012) on characterization of purified E/S antigen of H. contortus by SDS-PAGE revealed a single band at 66 kDa; Clarke and Slocombe (1984) observed a single band corresponding to a molecular weight of 172,000 Da from exsheathing fluid and somatic extracts of H. contortus; Siamba et al (2012) observed 13 major peptide bands in SDS PAGE of infective L 3 larvae of H. contortus and observed a specific 46 kDa protein in unstressed parasites and 32 kDa protein in stressed ones.…”

Section: Resultsmentioning

confidence: 99%

Protein profiling of Haemonchus contortus found in sheep of Kashmir valley

2014

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Economic losses due to helminth parasites in sheep throughout the world are considerable. Haemonchus contortus is a blood sucking intestinal helminth that lives in the abomasum of small ruminants worldwide. This parasite can be devastating to producers as it causes decreased production levels due to clinical signs such as anaemia, edema and death. For isolation of the proteins of the parasite, a well defined methodology was adopted. The abomasae of sheep in which this parasite resides were collected from abattoirs of various districts and were then carried to laboratory for screening. In case of collection sites falling in far areas, the organs were screened on spot. The parasites were collected in normal saline, washed and stored in 0.05 M PBS with pH of 7.4 at 0°C. After refrigeration, frozen nematodes were thawed, homogenized and centrifuged at 1,000-15,000 rpm for 15 min. The supernatant was thus collected as a protein mixture and stored at -20°C. Protein concentration of the samples was estimated by Lowry method. The samples were then analyzed through PAGE and then through SDS-PAGE. Protein estimation of the samples was estimated to be 4.2 mg/ml. The processed parasite samples were then subjected to PAGE and SDS-PAGE to determine the presence of the proteins. It showed high concentration of proteins in its whole protein profile. The proteins were seen as continuous bands intermixing with each other in PAGE analysis. The present study revealed two bands of molecular weights-55 and 33 kDa in PAGE analysis. The proteins when analyzed through SDS-PAGE were mostly found in the range of 25-70 kDa. The SDS-PAGE analysis showed four prominent bands. These bands were of the molecular weights of 66, 40, 33 and 26 kDa. The present work was a challenging one since only a single study was conducted in this region on this aspect and thus obviously was a big task to peep into the field where scanty input was available.

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“…Especialmente para os monócitos, sabe-se que o H. contortus adulto possui a habilidade de inibir a atuação destas células (RATHORE et al, 2006).…”

Section: Dargie E Allonby (1975) Trabalhando Com Infecção Experimentaunclassified

<b>Resistência, resiliência e sensibilidade de ovinos ao <i>Haemonchus contortus</i>:</b> comparações hematológicas e bioquímicas

Storillo¹

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Identification of a 66kDa Haemonchus contortus excretory/secretory antigen that inhibits host monocytes

Cited by 34 publications

References 21 publications

Haemonchus contortusexcretory and secretory proteins (HcESPs) suppress functions of goat PBMCsin vitro

Haemonchus contortusexcretory and secretory proteins (HcESPs) suppress functions of goat PBMCsin vitro

Protein profiling of Haemonchus contortus found in sheep of Kashmir valley

<b>Resistência, resiliência e sensibilidade de ovinos ao <i>Haemonchus contortus</i>:</b> comparações hematológicas e bioquímicas

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