Identification of a bacterial strain isolated from the liver of a laboratory mouse as Microbacterium paraoxydans and emended description of the species Microbacterium paraoxydans Laffineur et al 2003

Abstract

A rod-shaped, Gram-positive bacterial strain, designated C57-33, was isolated from the liver of the laboratory mouse strain C57Bl/6J and characterised by a polyphasic approach. 16S rRNA gene sequence similarity placed strain C57-33 in the genus Microbacterium with Microbacterium paraoxydans CF36T as the next relative (99.9 % sequence similarity). Major fatty acids ai-C 15:0 , i-C 16:0 and ai-C 17:0 and peptidoglycan type B2β with ornithine as the diagnostic cell-wall diamino acid and glycolyl residues were in agreement with the description of Microbacterium paraoxydans. The quinone system of C57-33 (major menaquinones MK-12 and MK-11) and polar lipid profi le (major polar lipids diphosphatidyl glycerol, phosphatidyl glycerol and two unknown glycolipids) were in accordance with those of Microbacterium paraoxydans strains CF36 T , CF7 and CF40 which were analysed in this study as well. The results of biochemical/physiological characterisation, DNA-DNA hybridization, MALDI-TOF mass spectrometry of cell extracts and comparison of protein patterns after SDS-PAGE demonstrated that our isolate C57-33 (= DSM 15461) is a strain of the species Microbacterium paraoxydans. Based on new characteristics such as quinone system, polar lipid profi le and physiological traits analysed for strain C57-33, the type strain of Microbacterium paraoxydans and some additional strains an emended description of the species Microbacterium paraoxydans is provided.

The abundant occurrence of hydrocarbon-utilizing bacteria in the phyllospheres of cultivated and wild plants in Kuwait

“…The few earlier studies, including ours, on leaf associated hydrocarbon-utilizing bacteria (Buczolits et al, 2008;Al-Mailem et al, 2010;McGuinness and Dowling, 2011;Sorkhoh et al, 2011;Ali et al, 2012) were done on few plant species, a shortcoming which has been corrected in this work. In this context, although the culture based approach for bacterial counting reveals only a fraction of the values obtained by the direct and molecular approaches, we adopted the culture based technique because, in contrast to the others, it specifically counts hydrocarbon utilizing bacteria, and makes bacterial cultures available for further study.…”

Microbacterium agarici sp. nov., Microbacterium humi sp. nov. and Microbacterium pseudoresistens sp. nov., isolated from the base of the mushroom Agaricus blazei

“…Strains CC-SBCK-209 T , CC-12309 T and CC-5209 T exhibited polar lipid profiles consisting of the major components diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid that showed identical behaviour during TLC analyses of the extracts of the three strains. These major polar lipids have been detected in numerous Microbacterium species (Zlamala et al, 2002;Rivas et al, 2004;Kim et al, 2005;Shivaji et al, 2007;Buczolits et al, 2008;Park et al, 2006) and they have also been listed in the emended description of the genus (Takeuchi & Hatano, 1998). In addition, in the extract from strain CC-SBCK-209 T , minor amounts of a second unknown glycolipid and two unknown phospholipids were detected (Fig.…”

“…The quinone system of strain CC-5209 T comprised MK-13 (92 %) as the major compound, with minor amounts of MK-12 (4 %) and MK-14 (4 %). It can be assumed that examination of additional strains of any of these species would show very similar quinone systems, as it has been shown that the relative amounts of the major quinones for four strains of Microbacterium paraoxydans are quite stable (Buczolits et al, 2008). Strains CC-SBCK-209 T , CC-12309 T and CC-5209 T exhibited polar lipid profiles consisting of the major components diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid that showed identical behaviour during TLC analyses of the extracts of the three strains.…”

Proposed minimal standards for describing new genera and species of the suborder Micrococcineae

“…Ribotyping and methods based on whole genomes are especially recommended for the examination of whether strains belong to the same species. However, it has been shown recently for Microbacterium paraoxydans that ribotyping does not identify all strains of this species (Buczolits et al, 2008), demonstrating that, as with other fingerprinting techniques, highly similar banding patterns are useful for species identification whereas different patterns do not necessarily indicate another species. However, further standardization of these techniques is required in order to improve the reproducibility between laboratories before these methods can be proposed as alternative minimal criteria that are equivalent to DNA-DNA hybridization.…”