Identification of a bio-signature for barley resistance against Pyrenophora teres infection based on physiological, molecular and sensor-based phenotyping

Pandey, Chandra Mouli; Großkinsky, Dominik K.; Westergaard, Jesper Cairo; Jørgensen, Hans Jørgen Lyngs; Svensgaard, Jesper; Christensen, Svend; Schulz, Alexander; Roitsch, Thomas

doi:10.1016/j.plantsci.2021.111072

Cited by 10 publications

(10 citation statements)

References 91 publications

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“…Our results showed that compared to the control (non-inoculated plants), CAT, POD, and SOD activities increased more after C. gloeosporioides infection in the disease-resistant lines than in the susceptible lines. These results are consistent with the previous studies ( Li et al., 2018 ; Pandey et al., 2021 ). There are two potential possibilities for disease-resistant lines to have higher conserved enzyme activities than disease-susceptible lines.…”

Section: Discussionsupporting

confidence: 94%

Overexpression of dihydroflavonol 4-reductase (CoDFR) boosts flavonoid production involved in the anthracnose resistance

Yang

Cao

et al. 2022

Front. Plant Sci.

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The outbreak of anthracnose caused by Colletotrichum spp. represents a devastating epidemic that severely affects oil tea (Camellia oleifera) production in China. However, the unknown resistance mechanism to anthracnose in C. oleifera has impeded the progress of breeding disease-resistant varieties. In this study, we investigated the physiological responses of resistant and susceptible lines during C. gloeosporioides infection. Our results showed that the accumulation of malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD), and peroxidase (POD) in both disease-resistant and susceptible lines increased by C. gloeosporioides infection. Also, disease-resistant lines exhibited lower MDA, but higher POD, SOD, and CAT activities compared to susceptible lines. The accumulation of flavonoids in both resistant and susceptible C. oleifera leaves increased following C. gloeosporioides infection, and the increase was greater in resistant lines. Further, we identified and functionally characterized the dihydroflavonol 4-reductase (CoDFR) from the resistant C. oleifera line. We showed that the full-length coding sequence (CDS) of CoDFR is 1044 bp encoding 347 amino acids. The overexpression of CoDFR in tobacco altered the expression of flavonoid biosynthetic genes, resulting in an increased flavonoid content in leaves. CoDFR transgenic tobacco plants exhibited increased anthracnose resistance. Furthermore, the transgenic plants had higher salicylic acid content. These findings offer potential insights into the pivotal role of CoDFR involved in flavonoid-mediated defense mechanisms during anthracnose invasion in resistant C. oleifera.

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Section: Discussionsupporting

confidence: 94%

Overexpression of dihydroflavonol 4-reductase (CoDFR) boosts flavonoid production involved in the anthracnose resistance

Yang

Cao

et al. 2022

Front. Plant Sci.

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“…"Antonella", which is the most resistant to PTT, had the lowest SOD activity in all isolates. However, this coincides with the findings that reported a positive association between the presence of ROS and disease resistance [12,55,56].…”

Section: Discussionsupporting

confidence: 92%

“…The previous results are in contradiction with those of Lightfoot et al [12], who detected higher SOD activity in resistant barley genotypes, and smaller symptoms were detected in transgenic HcCSD1 knock-down lines. However, when the expression pattern of SOD2 gene was investigated, it was found that except for clear activation of expression at 8 h after infection measured in the resistant genotype, the expression pattern fluctuated more in the sensitive ones, and there was a second expression peak at 120 h in resistance one after a decline at 48 h [56].…”

Section: Discussionmentioning

confidence: 98%

The Stimulation of Superoxide Dismutase Enzyme Activity and Its Relation with the Pyrenophora teres f. teres Infection in Different Barley Genotypes

et al. 2022

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Changes in superoxide dismutase (SOD) enzyme activity were examined in infected barley seedlings of five cultivars with the goal to study the role of SOD in the defense mechanism induced by Pyrenophora teres f. teres (PTT) infection. Our results showed that although there were differences in the responses of the cultivars, all three PTT isolates (H-618, H-774, H-949) had significantly increased SOD activity in all examined barley varieties at the early stages of the infection. The lowest SOD activity was observed in the case of the most resistant cultivar. Our results did not show a clear connection between seedling resistance of genotypes and SOD enzyme activity; however, we were able to find strong significant correlations between the PTT infection scores on the Tekauz scale and the SOD activity. The measurement of the SOD activity could offer a novel perspective to detect the early stress responses induced by PTT. Our results suggest that the resistance of varieties cannot be estimated based on SOD enzyme activity alone, because many antioxidant enzymes play a role in fine-tuning the defense response, but SOD is an important member of this system.

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“…Automated phenotyping with the help of UAVs or robotics can increase the number of measuring times compared with manual handling by traditional scoring based on visual inspection. Some pathogen infections only become visible by the naked eye at later stages of plant disease development, but can instead be captured pre-symptomatically by multispectral, hyperspectral, multicolour fluorescence, and PAM chlorophyll fluorescence imaging ( Hupp et al , 2019 ; Pandey et al , 2021 ). In spite of these emerging possibilities, there are so far relatively few studies in agricultural fields of disease progression using proximal or remote sensing in Nordic conditions, and a functional phenomics approach has not been taken.…”

Section: Plant Health and Crop Management In Nordic Conditionsmentioning

confidence: 99%

“…The Centre for Plant Research in Controlled Climate (SKP), in Ås, South Norway, a joint unit between the NMBU (Norwegian University of Life Sciences) and NIBIO, also offers a climate-controlled facility for research purposes. Automated HTP in the PhenoLab at the University of Copenhagen allows assessment of the impact of various abiotic environmental factors including expected future CO 2 levels on plant responses by multireflectance, fluorescence, and thermography ( Pandey et al , 2021 ). Finally, the Umeå Plant Science Centre hosts a unique conveyor belt-driven tree phenotyping platform which automatically monitors and records growth parameters.…”

Section: Functional Phenomics and Phenotyping Facilitiesmentioning

confidence: 99%

Functional phenomics for improved climate resilience in Nordic agriculture

Roitsch

Himanen

Chawade

et al. 2022

Journal of Experimental Botany

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The five Nordic countries span the most northern region for field cultivation in the world. This presents challenges per se with short growing seasons, long days and a need for frost tolerance. Climate change has additionally increased risks for micro-droughts and water logging as well as pathogens and pests expanding northwards. Thus, Nordic agriculture demands crops that are adapted to the special Nordic growth conditions and future climate scenarios. A focus on crop varieties and traits important to Nordic agriculture, including the unique resource of nutritious wild crops, can meet these needs. In fact, with a future longer growing season due to climate change the region could contribute proportionally more to the global agricultural production. This also applies to other northern regions, including the Arctic. To address current growth conditions, mitigate impacts of climate change and meet market demands, the adaptive capacity of crops that both perform well in northern latitudes and are more climate resilient has to be increased, and better crop management systems be built. This requires functional phenomics approaches that integrate versatile high-throughput phenotyping, physiology and bioinformatics. This review stresses key target traits, the opportunities of latitudinal studies and infrastructure needs for phenotyping to support Nordic agriculture.

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Identification of a bio-signature for barley resistance against Pyrenophora teres infection based on physiological, molecular and sensor-based phenotyping

Cited by 10 publications

References 91 publications

Overexpression of dihydroflavonol 4-reductase (CoDFR) boosts flavonoid production involved in the anthracnose resistance

Overexpression of dihydroflavonol 4-reductase (CoDFR) boosts flavonoid production involved in the anthracnose resistance

The Stimulation of Superoxide Dismutase Enzyme Activity and Its Relation with the Pyrenophora teres f. teres Infection in Different Barley Genotypes

Functional phenomics for improved climate resilience in Nordic agriculture

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