Identification of a C-type lectin possessing both antibacterial and antiviral activities from red swamp crayfish

Zhang, Xiaowen; Man, Xin; Huang, Xin; Wang, Yue; Song, Qisheng; Hui, Kai-Min; Zhang, Hongwei

doi:10.1016/j.fsi.2018.03.015

Cited by 40 publications

(13 citation statements)

References 37 publications

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“…The highest expression of PtCLec2 in intestine suggests that intestine might participate in the crustacean immune response. The expression levels of PtCLec2 were relatively higher in gill and eyestalk, which was also reported in CgCLec-3 from C. gigas [ 27 ] and PcLec6 from P. clarkii [ 28 ]. These results indicate that PtCLec2 could take part in the immune defense of gill and eyestalk.…”

Section: Discussionmentioning

confidence: 99%

“…The opsonization in hemolymph could contribute to the resistance of invading bacteria and activate the phagocytic efficiency [ 45 ]. Coincident with FmLdlr from F. merguiensis [ 12 ] and PcLec6 from P. clarkii [ 28 ], rPtCLec2 could directly promote the clearance efficiency against V. alginolyticus in vivo. Furthermore, rPtCLec2 could substantially increase the phagocytic activity of hemocytes, and RNAi of PtCLec2 could result in a lower expression of phagocytosis-associated genes, in accordance with reports on SpCTL-B from S. paramamosain [ 46 ] and PcLec3 from P. clarkii [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

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A C-Type Lectin Highly Expressed in Portunus trituberculatus Intestine Functions in AMP Regulation and Prophenoloxidase Activation

Liu

Zhang

et al. 2021

Antibiotics

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A C-type lectin (PtCLec2) from Portunus trituberculatus was identified for characterization of its role in defense and innate immunity. PtCLec2 contains a single carbohydrate-recognition domain (CRD) with a conserved QPD motif, which was predicted to have galactose specificity. The mRNA expression of PtCLec2 was predominantly detected in intestine and increased rapidly and significantly upon pathogen challenge. The recombinant PtCLec2 (rPtCLec2) could bind various microorganisms and PAMPs with weak binding ability to yeast and PGN. It agglutinated the tested Gram-negative bacteria (Vibrio alginolyticus and Pseudomonas aeruginosa), Gram-positive bacteria (Staphylococcus aureus and Micrococcus luteus), and rabbit erythrocytes in the presence of exogenous Ca2+, and these agglutination activities were suppressed by LPS, d-galactose, and d-mannose. Further, rPtCLec2 enhanced phagocytosis and clearance of V. alginolyticus, and displayed inhibitory activities against the tested bacteria. Knockdown of PtCLec2 decreased the transcription of two phagocytosis genes (PtArp and PtMyosin), three prophenoloxidase (proPO) system-related genes (PtPPAF, PtcSP1, and PtproPO), six antimicrobial peptides (AMPs) (PtALF4-7, PtCrustin1, and PtCrustin3), and PtRelish but upregulated the expression levels of PtJNK, PtPelle, and PtTLR. These results collectively indicate that PtCLec2 might perform its immune recognition function via binding and agglutination, and mediate pathogen elimination via regulating hemocyte phagocytosis, AMP synthesis, and proPO activation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

A C-Type Lectin Highly Expressed in Portunus trituberculatus Intestine Functions in AMP Regulation and Prophenoloxidase Activation

Liu

Zhang

et al. 2021

Antibiotics

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“…Since the same range is routinely injected in small and large species, the calculated sensitivity to RNAi appears lower in small species [42]. Similar phenomena exist in crustaceans [25,43,44,45]. Therefore, establishing a clear dose-to-interference response and determining the optimal interference dose and timing are critical to the success of RNAi technology.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Functional Study of Insulin-Like Androgenic Gland Hormone Gene in the Red Swamp Crayfish, Procambarus clarkii

Shi

Han

Fei

et al. 2019

Genes

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The androgenic gland (AG) is a male-specific endocrine organ that controls the primary and secondary sexual characteristics in male crustaceans. More evidence indicates that the insulin-like androgenic gland hormone gene (IAG) is the key male sexual differentiation factor, particularly the application of RNA interference (RNAi) technology on IAG. In this study, the full-length cDNA of IAG (termed PcIAG) was isolated from the red swamp crayfish, Procambarus clarkii. Tissue distribution analysis showed that in addition to its expression in the AG of male P. clarkii, PcIAG was widely expressed in female tissues and other male tissues. The PcIAG protein was detected in the reproductive and nervous systems of adult male P. clarkii. Additionally, RNAi results showed that the PcIAG expression could be silenced efficiently, and the male sperm maturation and release possibly present a transient adverse interference at lower doses (0.1 μg/g and 1 μg/g) of PcIAG–dsRNA (PcIAG double-stranded RNA). Dramatically, the expression level of PcIAG increased sharply shortly after the injection of higher doses (5 μg/g and 10 μg/g) of PcIAG–dsRNA, which might accelerate the maturation and release of sperm. Moreover, the expression of PcSxl (P. clarkii Sex-lethal) was detected by Quantitative Real-Time PCR (qPCR) after the injection of PcIAG–dsRNA to explore whether the PcIAG gene regulates the PcSxl gene, and we found that the PcIAG did not directly regulate PcSxl in P. clarkii. The study could help accelerate the progress of PcIAG functional research and provide a useful reference for the single-sex selective breeding of P. clarkii.

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“…The enzyme‐linked immunosorbent assay (ELISA) was used to detect the direct binding of rMaCTL to polysaccharides, referring to a previous study (Zhang et al., 2018). The polysaccharides used in this assay, including LPS from Escherichia coli 055:B5 and PGN from S. aureus , were purchased from Sigma.…”

Section: Methodsmentioning

confidence: 99%

“…The six kinds of bacteria mentioned above were used to evaluate the bacterial binding capacity of rMaCTL. The bacterial binding assay was performed referring to a previous study (Zhang et al., 2018). Each bacterium was cultured in LB broth overnight and then collected by centrifugation.…”

Section: Methodsmentioning

confidence: 99%

A C‐type lectin with antibacterial activity in weather loach, Misgurnus anguillicaudatus

Zhang

Yang

Zhang³

et al. 2020

Journal of Fish Diseases

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C‐type lectins are carbohydrate‐binding proteins that play important roles in immunity by serving as pattern recognition receptors. In the present study, a novel nattectin‐like C‐type lectin was obtained from the weather loach, Misgurnus anguillicaudatus, designated as MaCTL. MaCTL encodes a peptide with 165 amino acids, with a signal peptide and a single C‐type lectin domain (CTLD), containing a galactose‐specific QPD motif and a conserved Ca2+‐binding site. Transcripts of MaCTL were significantly upregulated after immune challenge with its pathogen A. hydrophila. In vitro assays with recombinant MaCTL protein revealed that it exhibited hemagglutinating and bacterial agglutinating activities, in a Ca2+‐dependent manner. MaCTL was found to bind to a wide range of bacteria, as well as bind to bacterial polysaccharides LPS and PGN. Moreover, MaCTL displayed antimicrobial activity by inhibiting the growth of bacteria. These results collectively suggest that MaCTL is involved in the antibacterial defence of weather loach.

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Identification of a C-type lectin possessing both antibacterial and antiviral activities from red swamp crayfish

Cited by 40 publications

References 37 publications

A C-Type Lectin Highly Expressed in Portunus trituberculatus Intestine Functions in AMP Regulation and Prophenoloxidase Activation

A C-Type Lectin Highly Expressed in Portunus trituberculatus Intestine Functions in AMP Regulation and Prophenoloxidase Activation

Molecular Characterization and Functional Study of Insulin-Like Androgenic Gland Hormone Gene in the Red Swamp Crayfish, Procambarus clarkii

A C‐type lectin with antibacterial activity in weather loach, Misgurnus anguillicaudatus

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