Identification of a cell line producing high levels of TSLP: Advantages for screening of anti-allergic drugs

Segawa, Ryosuke; Yamashita, Saori; Mizuno, Natsumi; Shiraki, Motohiro; Hatayama, Takahiro; Satou, Nozomi; Hiratsuka, Masahiro; Hide, Michihiro; Hirasawa, Noriyasu

doi:10.1016/j.jim.2013.10.012

Cited by 19 publications

(13 citation statements)

References 28 publications

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“…TSLP also induces the expression of B-cell activating factor by DCs, which promotes class switching by B-cells [ 27 , 28 ]. TSLP and IL-33 are primarily produced by epithelial cells [ 29 , 30 ]. Since CD11c-mediated Ig production by CDN-adjuvanted vaccine is abrogated in Tmem173 −/− mice compared to WT [ 6 ], CD11c + DCs are responsible for the STING-dependent adjuvanticity.…”

Section: Discussionmentioning

confidence: 99%

cGAMP Promotes Germinal Center Formation and Production of IgA in Nasal-Associated Lymphoid Tissue

et al. 2017

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Induction of immunoglobulin (Ig) A in the mucosa of the upper respiratory tract and the nasal cavity protects against influenza virus infection. Cyclic dinucleotides (CDNs) are used as mucosal adjuvants to enhance the immunogenicity of intranasal influenza hemagglutinin (HA) vaccines. The adjuvant activity of 2′3′ cyclic guanosine monophosphate–adenosine monophosphate (cGAMP) on Ig production was investigated in nasal-associated lymphoid tissue (NALT), serum of wild-type C57BL/6J, and stimulator of interferon genes (STING)-deficient mice, which do not recognize cGAMP. Mice were vaccinated intranasally with a HA vaccine with or without the cGAMP adjuvant. IgA and IgG production, T-cell responses, germinal center formation, and cytokine expression in NALT were assayed. cGAMP enhanced IgA and IgG production, and promoted T-cell responses. Intranasal administration of cGAMP activated both NALT and systemic immune cells, induced a favorable cytokine environment for IgA induction, and promoted germinal center formation. The cGAMP effect was STING-dependent. Taken together, cGAMP as an HA vaccine adjuvant promoted a STING-dependent NALT environment suitable for the enhancement of IgA production.

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Section: Discussionmentioning

confidence: 99%

cGAMP Promotes Germinal Center Formation and Production of IgA in Nasal-Associated Lymphoid Tissue

et al. 2017

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“…Thymic stromal lymphopoietin (TSLP), a sensitive marker of epithelial inflammation, is shown to be harmful to the epidermal barrier [ 13 , 14 , 15 , 16 ]. In fact, TSLP is highly expressed in keratinocytes from AD patients, and plays critical roles in the induction and exacerbation of this chronic dermatitis [ 17 ]. TSLP acts in AD pathogenesis through several mechanisms: (1) enhancing CD4+ T-cell polarization into Th2 cells [ 16 , 18 , 19 , 20 , 21 ]; (2) promoting IL-33-independent innate lymphoid cell responses [ 22 ]; and (3) targeting dendritic cells and innate lymphoid cells [ 23 , 24 ].…”

Section: Introductionmentioning

confidence: 99%

Association between Stress and the HPA Axis in the Atopic Dermatitis

Lin

Zhong

Santiago

2017

IJMS

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The hypothalamic–pituitary–adrenal (HPA) axis is one of the body’s neuroendocrine networks that responds to psychological stress (PS). In the skin, there exists a peripheral HPA axis similar to the central axis. Glucocorticoids (GCs) are key effector molecules of the HPA axis and are essential for cutaneous homeostasis. Atopic dermatitis (AD) is a condition typically characterized by a chronic relapsing course that often results in PS. HPA dysfunction is present in AD patients by the decreased response of GCs elevation to stress as compared to those unaffected by AD. Nevertheless, in skin, acute PS activates several metabolic responses that are of immediate benefit to the host. During the acute phase of PS, increased endogenous GCs have been shown to provide benefit rather than by aggravating cutaneous inflammatory dermatoses. However, a chronic T helper cell type 2 (Th2) predominant cytokine profile acts as a negative feedback loop to blunt the HPA axis response in AD. In this article, we reviewed the role of CRF, pro-opiomelanocortin (POMC)-derived peptides, GCs of the HPA, and 11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) in AD, with a discussion of the pathogenetic mechanisms of inflammation and skin barrier functions, including antimicrobial defense, and their association with PS.

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“…Here, we investigated whether dieckol, a phlorotannin from Ecklonia cava (Figure 1a), can suppress TSLP production to reduce Th2 immunity and effectively alleviate AD-like symptoms in an NC/Nga mouse model in vivo. First, we determined whether dieckol affects the production of TSLP in a mouse keratinocyte cell line, KCMH-1, that constitutively produces high amounts of TSLP (Segawa et al, 2014). Dieckol reduced TSLP mRNA and protein levels in KCMH-1 cells (Figure 1b and c).…”

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confidence: 99%

Topical Application of Dieckol Ameliorates Atopic Dermatitis in NC/Nga Mice by Suppressing Thymic Stromal Lymphopoietin Production

Yang

Lee

et al. 2016

Journal of Investigative Dermatology

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Identification of a cell line producing high levels of TSLP: Advantages for screening of anti-allergic drugs

Cited by 19 publications

References 28 publications

cGAMP Promotes Germinal Center Formation and Production of IgA in Nasal-Associated Lymphoid Tissue

cGAMP Promotes Germinal Center Formation and Production of IgA in Nasal-Associated Lymphoid Tissue

Association between Stress and the HPA Axis in the Atopic Dermatitis

Topical Application of Dieckol Ameliorates Atopic Dermatitis in NC/Nga Mice by Suppressing Thymic Stromal Lymphopoietin Production

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