Identification of a Circulating MicroRNA Signature for Colorectal Cancer Detection

Wang, Jia; Huang, Shiang Suo; Zhao, Mei; Yang, Mei; Zhong, Jialing; Gu, Yating; Peng, Hua; Che, Yi-Qun; Huang, Changzhi

doi:10.1371/journal.pone.0087451

Cited by 150 publications

(121 citation statements)

References 35 publications

Supporting

Mentioning

112

Contrasting

Unclassified

Order By: Relevance

“…In addition, this signature could be used in combination with other noninvasive biomarkers. Several research groups showed a rise in sensitivity by combining microRNA signatures with the detection of the carcinoembryonic antigen (CEA) [23,48]. Thus, in combination with other potential tumor markers this five microRNA signatures could be a powerful tool in the noninvasive diagnostic of lung cancer.…”

Section: Discussionmentioning

confidence: 99%

Extracellular microRNAs in bronchoalveolar lavage samples from patients with lung diseases as predictors for lung cancer

Rehbein

Schmidt

Fleischhacker

2015

Clinica Chimica Acta

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Extracellular microRNAs in bronchoalveolar lavage samples from patients with lung diseases as predictors for lung cancer

Rehbein

Schmidt

Fleischhacker

2015

Clinica Chimica Acta

View full text Add to dashboard Cite

“…There is some evidence suggesting that circulating miRNAs can contribute to tumorigenesis by modulating oncogenic or tumor suppressor pathways 22,10 . Several studies have compared the expression profiles of miRNAs in serum or plasma across a variety of tumours to identify cancer-specific expression patterns: data obtained suggested that circulating miRNAs expression could be used to discriminate disease samples, demonstrating their potential use as blood-based diagnostic cancer markers [23][24][25][26] .…”

Section: Mirna Alterations In Um Vh and Exosomes Significantly Overlapmentioning

confidence: 99%

miRNA profiling in vitreous humor, vitreal exosomes and serum from uveal melanoma patients: Pathological and diagnostic implications

Ragusa

Barbagallo

Statello

et al. 2015

Cancer Biology & Therapy

141

143

View full text Add to dashboard Cite

Uveal melanoma (UM) represents approximately 5-6% of all melanoma diagnoses and up to 50% of patients succumb to their disease. Although several methods are available, accurate diagnosis is not always easily feasible because of potential accidents (e.g., intraocular hemorrhage). Based on the assumption that the profile of circulating miRNAs is often altered in human cancers, we verified whether UM patients showed different vitreous humor (VH) or serum miRNA profiles with respect to healthy controls. By using TaqMan Low Density Arrays, we analyzed 754 miRNAs from VH, vitreal exosomes, and serum of 6 UM patients and 6 healthy donors: our data demonstrated that the UM VH profile was unique and only partially overlapping with that from serum of the same patients. Whereas, 90% of miRNAs were shared between VH and vitreal exosomes, and their alterations in UM were statistically overlapped with those of VH and vitreal exosomes, suggesting that VH alterations could result from exosomal dysregulation. We report 32 miRNAs differentially expressed in UM patients in at least 2 different types of samples analyzed. We validated these data on an independent cohort of 12 UM patients. Most alterations were common to VH and vitreal exosomes (e.g., upregulation of miR-21,-34 a,-146a). Interestingly, miR-146a was upregulated in the serum of UM patients, as well as in serum exosomes. Upregulation of miR-21 and miR-146a was also detected in formalin-fixed, paraffin-embedded UM, suggesting that VH or serum alterations in UM could be the consequence of disregulation arising from tumoral cells. Our findings suggest the possibility to detect in VH and serum of UM patients "diagnostic" miRNAs released by the affected eye: based on this, miR-146a could be considered a potential circulating marker of UM.

show abstract

“…candidate biomarkers for CRC diagnosis, miR-92a was down-regulated in CRC than control samples [18]. The similar contradictory results were also observed in the circulating miRNAs miR-145, miR-150 and miR-203 [19][20][21][22].…”

Section: Main Circulating Mirnas In Crc Diagnosismentioning

confidence: 54%

Circulating MicroRNAs in Colorectal Cancer

Huang¹,

Ge²,

Hu³

et al. 2017

J Mol Genet Med

View full text Add to dashboard Cite

IntroductionWith increasing incidence, colorectal cancer (CRC) remains the second most common cancer in women and the third in men, despite the improved screening techniques, most patients diagnosed CRC are at late stage, CRC is the second leading cause of cancer-related death worldwide [1]. Complete tumor resection by surgery is the main therapeutic modality, it was reported that 5-year survival rates were in range from 93.2% for the earliest stage to 6.6% for advanced disease at diagnosis according to Duke's stage. So, good prognosis of CRC is linked to stage at earlier diagnosis. However, due to asymptomatic in early stage of CRC, many cases were detected at late stage. In clinical practice, colonoscopy is used in the diagnosis and treatment of CRC, but has not yet been wide-used as a screening tool because of some limitations, such as the requirements of bowel preparation, cost burden and perforation risk [2]. In addition, fecal occult blood test (FOBT), some convenient circulating blood biomarkers, including the most frequently used marker carcinoembryonic antigen (CEA), carbohydrate antigens, CA125, CA153 and CA199, are lack of sufficient sensitivity or specificity. For example, the specificity of FOBT is about 95%, while the sensitivity of is around 70% to 75% [3], the sensitivity and specificity of CEA is 60% and 34%, respectively [4]. Therefore, new strategies and novel biomarkers with the characters of detection, staging and prediction of outcome are highly desirable and being explored in order to make optimized treatment prescription and to improve prognosis for CRC patients.A growing number of researches focus on small non-coding RNA molecules, microRNAs (miRNAs, miRs), which play multiple roles in variety of biological processes, including cancer [5][6][7]. Since 2008, tumor-derived miRNAs were described present stablely in circulating blood, as circulating-based markers for cancer detection [8]. Consider the detection of circulating miRNAs have the advantages of simple, inexpensive and noninvasive, combined with the remarkable stability characteristics of mature miRNAs due to the miRNA-Argonaute-protein complex, the discovery of circulating miRNAs as novel biomarkers for cancer intervention has been widely explored [9,10]. Growing evidences have indicated that aberrant expression of circulating miRNAs has an association with cancer including CRC. The diagnostic and prognostic value of circulating miRNAs exhibited in screening and monitoring, predicting recurrence or metastasis including lymph node, vessel, peritoneal invasion or distant metastasis, stratification by TNM stage or even histological differentiation grade, and few are associated with drug resistance, chemoradiosensitivity, tumor size and gender. This review covers recent researches in circulating miRNAs and their variety potential values correlated with CRC (Figure 1). Experiment FlowExperiment flow for the identification of CRC-related circulating miRNAs apply step-wise strategy. First experiment samples are selected according to dif...

show abstract

Identification of a Circulating MicroRNA Signature for Colorectal Cancer Detection

Cited by 150 publications

References 35 publications

Extracellular microRNAs in bronchoalveolar lavage samples from patients with lung diseases as predictors for lung cancer

Extracellular microRNAs in bronchoalveolar lavage samples from patients with lung diseases as predictors for lung cancer

miRNA profiling in vitreous humor, vitreal exosomes and serum from uveal melanoma patients: Pathological and diagnostic implications

Circulating MicroRNAs in Colorectal Cancer

Contact Info

Product

Resources

About