Identification of a family of endocytic proteins that define a new α‐adaptin ear‐binding motif

Abstract

Endocytosis by clathrin-coated vesicles (CCVs

Gene expression profiles modulated by the human carcinogen aristolochic acid I in human cancer cells and their dependence on TP53

“…NECAP1 has been shown to play a role in clathrin-mediated endocytosis, although its precise function is unclear (Ritter et al, 2003). A function of RAB5A is to regulate endosome fusion, but a key role for this small GTPase in the initial phases of receptor-mediated endocytosis is also emerging (Lanzetti et al, 2001;Christensen et al, 2003).…”

Canonical Interaction of Cyclin G–associated Kinase with Adaptor Protein 1 Regulates Lysosomal Enzyme Sorting

“…Consistent with this structural similarity, all five domains bind with various affinities to a common set of "accessory proteins," including rabaptin-5 (Hirst et al, 2000;Doray and Kornfeld, 2001;Shiba et al, 2002;Mattera et al, 2003), ␥-synergin (Page et al, 1999;Hirst et al, 2000;Takatsu et al, 2000), p56 Lui et al, 2003), NECAP1 and NECAP2 (Ritter et al, 2003;Mattera et al, 2004), aftiphilin (Mattera et al, 2004), ␥-BAR (Neubrand et al, 2005), and a protein known as Clint, enthoprotin, or epsinR (Kalthoff et al, 2002;Wasiak et al, 2002;Hirst et al, 2003;Mills et al, 2003). Rabaptin-5 is part of a complex with the Rab5 guanine-nucleotide-exchange factor, rabex-5 (Horiuchi et al, 1997;, whereas ␥-synergin and aftiphilin form a complex with another protein named p200 (Hirst et al, 2005).…”

ENTH/ANTH proteins and clathrin-mediated membrane budding

“…Additionally, AP-2 recruits a diverse array of endocytic accessory proteins to sites of CCV formation (Slepnev and De Camilli, 2000;Takei and Haucke, 2001). These proteins bind through short consensus peptide motifs to a globular domain at the C-terminus of the AP-2 α-adaptin subunit termed the α-ear (Owen et al, 1999;Traub et al, 1999;Brett et al, 2002;Ritter et al, 2003).…”

“…Additionally, AP-2 recruits a diverse array of endocytic accessory proteins to sites of CCV formation (Slepnev and De Camilli, 2000;Takei and Haucke, 2001). These proteins bind through short consensus peptide motifs to a globular domain at the C-terminus of the AP-2 α-adaptin subunit termed the α-ear (Owen et al, 1999;Traub et al, 1999;Brett et al, 2002;Ritter et al, 2003).Members of the epsin family of endocytic regulatory proteins are important binding partners for the α-ear (Chen et al, 1998;Yamabhai et al, 1998;Nakashima et al, 1999;Rosenthal et al, 1999;Spradling et al, 2001) (Fig. 1).…”