Identification of a germ cell marker gene, the dead end homologue, in Chinese sturgeon Acipenser sinensis

Yang, Xiaoge; Yue, Huamei; Ye, Huan; Li, Chuangju; Wei, Qiwei

doi:10.1016/j.gene.2014.12.059

Cited by 32 publications

(17 citation statements)

References 34 publications

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“…The expression patterns of Ardnd were examined in several tissues of mature sterlet by RT-PCR in this study. The only signal was detected in gonads of both sexes, as was also reported in other fishes such as zebrafish [8], medaka [33], Atlantic salmon [36], Pacific bluefin tuna [20], turbot [12], and Chinese sturgeon [9]. This confirmed that dnd is a highly specific gene expressed in germ cells conserved in animals.…”

Section: Identification Of Dnd Genesupporting

confidence: 85%

“…Moreover, homology and identity searches of Agdnd with dead end homologue of the Chinese sturgeon Acipenser sinensis (Asdnd, KM 655832.1, [9]) (the only published dnd in acipenseridae family) were performed using the online Web site (http://www.ncbi.nlm.nih.gov/gene).…”

Section: Sequence Analysis and Alignmentmentioning

confidence: 99%

“…The dnd orthologs have already been described in invertebrate model organisms such as Drosophila melanogaster and Caenorhabditis elegans [8] and vertebrates such as chicken Gallus gallus domesticus [31], frog Xenopus laevis [8], mouse Mus musculus [32]. In fish, it has been initially identified in zebrafish Danio rerio [8] and subsequently in other teleosts such as medaka Oryzias latipes [33], loach Misgurnus anguillicaudatus [34], goldfish Carassius auratus [35], Atlantic salmon Salmo salar [36], Pacific bluefin tuna Thunnus orientalis [20], turbot Scophthalmus maximus [12], and the chondrosteans such as the Chinese sturgeon [9] and Russian sturgeon (Hagihara, unpublished data). The expression patterns of Ardnd were examined in several tissues of mature sterlet by RT-PCR in this study.…”

Section: Identification Of Dnd Genementioning

confidence: 99%

“…The Agdnd and Ardnd sequences in the ATG region were similar which presents the possibility of inhibiting the dnd translation in two sturgeon species Russian (discussed in the following) and sterlet (present work). Multiple alignments showed that Russian Agdnd shared 97% identity with sequenced fragments of Ardnd and with full-length Chinese sturgeon Asdnd (KM 655832.1, [9]). By comparing the phylogenetic trees of Agdnd and Asdnd, we can classify these genes in a separate clade, as we have called "acipenseridae," from fish and tetrapods.…”

Section: Identification Of Dnd Genementioning

confidence: 99%

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Sterilization of sterlet Acipenser ruthenus by using knockdown agent, antisense morpholino oligonucleotide, against dead end gene

et al. 2015

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Sturgeons (chondrostean, acipenseridae) are ancient fish species, widely known for their caviar. Nowadays, most of them are critically endangered. The sterlet (Acipenser ruthenus) is a common Eurasian sturgeon species with a small body size and the fastest reproductive cycle among sturgeons. Such species can be used as a host for surrogate production; application is of value for recovery of critically endangered and huge sturgeon species with an extremely long reproductive cycle. One prerequisite for production of the donor's gametes only is to have a sterile host. Commonly used sterilization techniques in fishes such as triploidization or hybridization do not guarantee sterility in sturgeon. Alternatively, sterilization can be achieved by using a temporary germ cell exclusion-specific gene by a knockdown agent, the antisense morpholino oligonucleotide (MO). The targeted gene for the MO is the dead end gene (dnd) which is a vertebrate-specific gene encoding a RNA-binding protein which is crucial for migration and survival of primordial germ cells (PGCs). For this purpose, a dnd homologue of Russian sturgeon (Agdnd), resulting in the same sequence in the start codon region with isolated fragments of sterlet dnd (Ardnd), was used. Reverse transcription polymerase chain reaction confirmed tissue-specific expression of Ardnd only in the gonads of both sexes. Dnd-MO for depletion of PGCs together with fluorescein isothiocyanate (FITC)-biotin-dextran for PGCs labeling was injected into the vegetal region of one- to four-cell-stage sterlet embryos. In the control groups, only FITC was injected to validate the injection method and labeling of PGCs. After optimization of MO concentration together with volume injection, 250-μM MO was applied for sterilization of sturgeon embryos. Primordial germ cells were detected under a fluorescent stereomicroscope in the genital ridge of the FITC-labeled control group only, whereas no PGCs were present in the body cavities of morphants at 21 days after fertilization. Moreover, the body cavities of MO-treated and nontreated fish were examined by histology and in situ hybridization, showing gonads which had no germ cells in morphants at various stages (60, 150, and 210 days after fertilization). Taken together, these results report the first known and functional method of sturgeon sterilization.

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Section: Identification Of Dnd Genesupporting

confidence: 85%

Section: Sequence Analysis and Alignmentmentioning

confidence: 99%

Section: Identification Of Dnd Genementioning

confidence: 99%

Section: Identification Of Dnd Genementioning

confidence: 99%

See 2 more Smart Citations

Sterilization of sterlet Acipenser ruthenus by using knockdown agent, antisense morpholino oligonucleotide, against dead end gene

et al. 2015

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“…It has also been investigated in teleost fish, for example, in zebrafish Danio rerio (Elkouby, ; Elkouby et al, ; Elkouby & Mullins, ; Lyman‐Gingerich & Pelegri, ; Marlow & Mullins, ), rainbow trout Salmo gairdneri (Beams & Kessel, ), golden grey mullet Mugil (Liza) auratus (Bruslé, ), medaka Oryzias latipes (Iwamatsu & Nakashima, ; Kobayashi & Iwamatsu, ; Nakamura et al, ), and in the Atlantic salmon Salmo salar (Škugor, Tveiten, Johnsen, & Andersen, ). It is also present in oocytes of several species of sturgeons and paddlefish (Chondrostei, Acipenseriformes); for example, in the Russian sturgeon, Acipenser gueldenstaedtii (Zelazowska et al, ; Żelazowska, Jankowska, Plewniak, & Rajek, ), Siberian sturgeon, A. baerii (Żelazowska & Fopp‐Bayat, , ), Chinese, A. sinensis , Dabry's, A. dabryanus sturgeons (Yang, Yue, Ye, Li, & Wei, ; Ye et al, ; Ye, Li, Yue, Yang, & Wei, ; Ye, Yue, Yang, Li, & Wei, ; Yue et al, ), the North American paddlefish, Polyodon spathula (Żelazowska et al, ; Żelazowska & Kilarski, ) as well as in numerous species of invertebrates (e.g., Bilinski et al, ; Kloc, Jedrzejowska, Tworzydlo, & Bilinski, ). In all species investigated, the Balbiani body is involved in the development of ooplasm polarity and marks the future vegetal region or vegetal pole in the oocytes.…”

Section: Introductionmentioning

confidence: 99%

Previtellogenic oocytes of South African largemouth bass Micropterus salmoides Lacépède 1802 (Actinopterygii, Perciformes) ‐ the Balbiani body, cortical alveoli and developing eggshell

Żelazowska

Halajian

2019

Journal of Morphology

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The ovaries of the largemouth bass Micropterus salmoides, an alien and invasive species in South Africa, contain a germinal epithelium which consists of germline and somatic cells, as well as previtellogenic and late vitellogenic ovarian follicles. The ovarian follicle consists of an oocyte surrounded by follicular cells and a basal lamina; thecal cells adjacent to this lamina are covered by an extracellular matrix. In this article, we describe the Balbiani body and the polarization and ultrastructure of the cytoplasm (ooplasm) in previtellogenic oocytes. The nucleoplasm in all examined oocytes contains lampbrush chromosomes, nuclear bodies and several nucleoli near the nuclear envelope. The ultrastructure of the nucleoli is described. Numerous nuage aggregations are present in the perinuclear cytoplasm in germline cells as well as in the ooplasm. Possible roles of these aggregations are discussed. The ooplasm contains the Balbiani body, which defines the future vegetal region in early previtellogenic oocytes. It is comprised of nuage aggregations, rough endoplasmic reticulum, Golgi apparatus, mitochondria, complexes of mitochondria with nuage‐like material, and lysosome‐like organelles. In mid‐previtellogenic oocytes, the Balbiani body surrounds the nucleus and later disperses in the ooplasm. The lysosome‐like organelles fuse and transform into vesicles containing material which is highly electron dense. As a result of the fusion of the vesicles of Golgi and rough endoplasmic reticulum, the cortical alveoli arise and distribute uniformly throughout the ooplasm of late previtellogenic oocytes. During this stage, the deposition of the eggshell (zona radiata) begins. The eggshell is penetrated by canals containing microvilli and consists of the following: the internal and the external egg envelope. In the external envelope three sublayers can be distinguished.

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Sex Determination in Sturgeon

Wuertz

Güralp²,

Pšenička³

et al. 2018

Sex Control in Aquaculture

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Identification of a germ cell marker gene, the dead end homologue, in Chinese sturgeon Acipenser sinensis

Cited by 32 publications

References 34 publications

Sterilization of sterlet Acipenser ruthenus by using knockdown agent, antisense morpholino oligonucleotide, against dead end gene

Sterilization of sterlet Acipenser ruthenus by using knockdown agent, antisense morpholino oligonucleotide, against dead end gene

Previtellogenic oocytes of South African largemouth bass Micropterus salmoides Lacépède 1802 (Actinopterygii, Perciformes) ‐ the Balbiani body, cortical alveoli and developing eggshell

Sex Determination in Sturgeon

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