“…These data indicate that a compound’s ferroptosis inhibiting activity in zebrafish hair cells largely predicts its activity in cell-based assay. Meanwhile, 15 out of the top 20 hits from our cell-based screen [ 44 ] are not otoprotective in vivo in neomycin-induced hair cell death assay, indicating ferroptosis inhibiting activity in cell-based assay does not guarantee otoprotective function in vivo, possibly due to factors such as compound stability, absorption, distribution, metabolism, toxicity, etc. Thus, positive hits from cell-based screen should be further validated in vivo in models such as zebrafish or mouse for their potential as candidate compounds for further drug development.…”