Identification of a growth phase-dependent promoter in the rplJL operon of Streptomyces coelicolor A3(2)

Blanco, Gloria; Sánchez, César; Rodicio, M. Rosario; Méndez, Cármen; Salas, José A.

doi:10.1016/s0167-4781(00)00280-3

Cited by 5 publications

(8 citation statements)

References 35 publications

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“…The rplJ and rplL genes lie together in an operon and encode the ribosomal protein subunits L10 and L7/L12. The same growth phase-dependent transcription of the rplJL operon showing a decrease at transition phase was already shown for another actinomycete, Streptomyces coelicolor [33]. The third gene in this category is sigA encoding the essential house-keeping sigma factor SigA.…”

Section: Resultssupporting

confidence: 61%

The alternative sigma factor SigB of Corynebacterium glutamicum modulates global gene expression during transition from exponential growth to stationary phase

et al. 2007

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Background: Corynebacterium glutamicum is a gram-positive soil bacterium widely used for the industrial production of amino acids. There is great interest in the examination of the molecular mechanism of transcription control. One of these control mechanisms are sigma factors. C. glutamicum ATCC 13032 has seven putative sigma factor-encoding genes, including sigA and sigB. The sigA gene encodes the essential primary sigma factor of C. glutamicum and is responsible for promoter recognition of house-keeping genes. The sigB gene codes for the non-essential sigma factor SigB that has a proposed role in stress reponse.

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Section: Resultssupporting

confidence: 61%

The alternative sigma factor SigB of Corynebacterium glutamicum modulates global gene expression during transition from exponential growth to stationary phase

et al. 2007

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“…Two dimensional gel electrophoresis has shown that production of ribosomal proteins in S. coelicolor varies with the phase of cell growth (Blanco et al 1994). Evidence that activity of the promoter for the rplJL operon, which encodes ribosomal proteins L10 and L7/L12, is growth-phase dependent (Blanco et al 2001), provides a possible mechanistic basis for the observed decrease in ribosomal proteins and transcripts.…”

Section: Microarray Analysis Of Streptomyces Transcription Genes and Dementioning

confidence: 99%

Global analysis of growth phase responsive gene expression and regulation of antibiotic biosynthetic pathways inStreptomyces coelicolorusing DNA microarrays

Huang¹,

Lih²,

Pan³

et al. 2001

Genes Dev.

195

188

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The eubacterial species Streptomyces coelicolor proceeds through a complex growth cycle in which morphological differentiation/development is associated with a transition from primary to secondary metabolism and the production of antibiotics. We used DNA microarrays and mutational analysis to investigate the expression of individual genes and multigene antibiotic biosynthetic pathways during these events. We identified expression patterns in biosynthetic, regulatory, and ribosomal protein genes that were associated highly specifically with particular stages of development. A knowledge-based algorithm that correlates temporal changes in expression with chromosomal position identified groups of contiguous genes expressed at discrete stages of morphological development, inferred the boundaries of known antibiotic synthesis gene loci, and revealed novel physical clusters of coordinately regulated genes. Microarray analysis of RNA from cells mutated in genes regulating synthesis of the antibiotics actinorhodin (Act) and undecylprodigiosin (Red) identified proximate and distant sites that contain putative ABC transporter and two-component system genes expressed coordinately with genes of specific biosynthetic pathways and indicated the existence of two functionally and physically discrete regulons in the Red pathway.

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“… # , estimated value (see text). † , data for E. coli B/r [20] . The value for n RNAP(rrs) was calculated using equations (1) and (2): n R(i)(av) and n R(j)(av) were calculated using equations (8) and (9); n tr(i)(av) and n tr(j)(av) were derived from n R(i)(av) and n R(j)(av) by means of n R(i)/tr(i) and n R(j)/tr(j) .…”

Section: Resultsmentioning

confidence: 99%

“…The calculations for both S. coelicolor A3(2) and M. bovis BCG are in accord with the data for E. coli B/r. The numbers of transcripts per cell were estimated to be 4.8 (0.5 per 1000 copies of 16S rRNA) and 9.6 (1 copy per 1000 copies of 16S rRNA) respectively for rpsL and rpl L for S. coelicolor A3(2) [20] . The numbers of transcripts per cell were estimated to be 1.3 (0.33 per 1000 copies of 16S rRNA) and 2.7(0.68 copies per 1000 copies of 16S rRNA) respectively for rpsL and rpl L for M.bovis BCG [18] .…”

Section: Resultsmentioning

confidence: 99%

“…The genes rpl J and rpl L form an operon in E. coli , Streptomyces coelicolor A3(2) and mycobacteria. The operon was found to have a single start site for transcription located upstream from rplJ in both E. coli [25] and S. coelicolor A3(2) [20] . Transcription was found to be autogenously controlled by the pentameric complex of one copy of rplJ with four copies of rplL binding to a site within the leader region of rpl J [26] .…”

Section: Discussionmentioning

confidence: 99%

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Measurement of the Rates of Synthesis of Three Components of Ribosomes of Mycobacterium fortuitum: A Theoretical Approach to qRT-PCR Experimentation

2010

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BackgroundExcept for the ribosomal protein L12 (rplL), ribosomal proteins are present as one copy per ribosome; L12 (rplL) is unusual because it is present as four copies per ribosome. Thus, the strategies used by Mycobacterium fortuitum to regulate ribosomal protein synthesis were investigated, including evaluations of the rates of chain elongations of 16S rRNA, rplL and ribosomal protein S12 (rpsL).MethodologyRNA was isolated from cell cultures and cDNA was prepared. The numbers of cDNA copies of 16S rRNA, precursor-16S rRNA and transcripts of rpsL and rplL were quantified by qRT-PCR and then related to the rates of 16S rRNA, rpsL and rplL chain elongations by means of a mathematical framework for coupled transcription/translation.Principal FindingsThe rates of synthesis of 16S rRNA, rpsL and rplL respectively were found to be approximately 50×103 nucleotides h−1, 1.6×103 amino acid residues h−1 and 3.4×103 amino acid residues h−1. The number of transcripts of rplL was approximately twice that of rpsL. These data account for the presence of one copy of rpsL and four copies of rplL per ribosome, and reveal that the rate of M. fortuitum ribosome synthesis was closer to that of M. tuberculosis than to E. coli. Except for rplJ, the elongation rate obtained for rpsL was inferred to be appropriate for all other proteins present as one copy per ribosome.SignificanceThe results obtained provide the basis for a comprehensive view of the kinetics of ribosome synthesis, and of the ways that bacterial cells utilize genes encoding ribosomal proteins. The methodology also applies to proteins involved in transcription, energy generation and to bacterial proteins in general. The method proposed for measuring the fidelity of cDNA preparations is intrinsically much more sensitive than procedures that measure the integrity of 16S rRNA.

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Identification of a growth phase-dependent promoter in the rplJL operon of Streptomyces coelicolor A3(2)

Cited by 5 publications

References 35 publications

The alternative sigma factor SigB of Corynebacterium glutamicum modulates global gene expression during transition from exponential growth to stationary phase

The alternative sigma factor SigB of Corynebacterium glutamicum modulates global gene expression during transition from exponential growth to stationary phase

Global analysis of growth phase responsive gene expression and regulation of antibiotic biosynthetic pathways inStreptomyces coelicolorusing DNA microarrays

Measurement of the Rates of Synthesis of Three Components of Ribosomes of Mycobacterium fortuitum: A Theoretical Approach to qRT-PCR Experimentation

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