Identification of a high frequency of chromosomal rearrangements in the centromeric regions of prostate cancer patients

Balachandar, V.; Kumar, B.V. Manoj; Sasikala, K.; Pappuswamy, Manikantan; Sangeetha, R.; Devi, Subramaniam Mohana

doi:10.1631/jzus.2007.b0638

Cited by 8 publications

(7 citation statements)

References 42 publications

(43 reference statements)

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“…As such, a recent study has demonstrated that HP1 variants display decreased expression levels in prostate cancer cell lines and prostate cancer tissues compared to normal tissues 32. In addition, an elevated frequency of chromosomal rearrangements is observed in the centromeric regions of prostate cancer patients,33 which is consistent with the loss of centromeric integrity associated with decreased levels of HP1 and heterochromatin stability. To investigate a potential involvement of KDM2A in prostate cancer, we searched the Oncomine on-line databases for differential KDM2A expression in normal versus tumorigenic prostate tissues.…”

Section: Resultsmentioning

confidence: 76%

KDM2A represses transcription of centromeric satellite repeats and maintains the heterochromatic state

Frescas¹,

Guardavaccaro²,

Kuchay³

et al. 2008

Cell Cycle

129

131

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Heterochromatin plays an essential role in the preservation of epigenetic information, the transcriptional repression of repetitive DNA elements and inactive genes and the proper segregation of chromosomes during mitosis. Here we identify KDM2A, a JmjC-domain containing histone demethylase, as a heterochromatin-associated protein that is required to maintain the heterochromatic state, as determined using both a candidate-based approach and an unbiased siRNA library screen. Moreover, we demonstrate that KDM2A represses transcription of small non-coding RNAs that are encoded by clusters of satellite repeats at the centromere. Finally, we show that KDM2A is required to sustain centromeric integrity and genomic stability, particularly during mitosis. Since the disruption of epigenetic control mechanisms contributes to cellular transformation, these results, together with the low levels of KDM2A found in prostate carcinomas, suggest a role for KDM2A in cancer development.

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Section: Resultsmentioning

confidence: 76%

KDM2A represses transcription of centromeric satellite repeats and maintains the heterochromatic state

Frescas¹,

Guardavaccaro²,

Kuchay³

et al. 2008

Cell Cycle

129

131

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“…13 The latter resides in the long arm of chromosome 9 that is frequently deleted in human cancers. 14, 15, 16 This prompted us to investigate whether the deletion of that region caused miR-204 downregulation in gastric tumoral tissues. To this end, we evaluated the presence of TRPM3 gene loss using a PCR-based test termed Multiplex Ligation-dependent Probe Amplification.…”

Section: Resultsmentioning

confidence: 99%

miR-204 targets Bcl-2 expression and enhances responsiveness of gastric cancer

Sacconi¹,

Biagioni²,

Canu³

et al. 2012

Cell Death Dis

162

138

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Micro RNAs (miRs) are small non-coding RNAs aberrantly expressed in human tumors. Here, we aim to identify miRs whose deregulated expression leads to the activation of oncogenic pathways in human gastric cancers (GCs). Thirty nine out of 123 tumoral and matched uninvolved peritumoral gastric specimens from three independent European subsets of patients were analyzed for the expression of 851 human miRs using Agilent Platform. The remaining 84 samples were used to validate miRs differentially expressed between tumoral and matched peritumoral specimens by qPCR. miR-204 falls into a group of eight miRs differentially expressed between tumoral and peritumoral samples. Downregulation of miR-204 has prognostic value and correlates with increased staining of Bcl-2 protein in tumoral specimens. Ectopic expression of miR-204 inhibited colony forming ability, migration and tumor engraftment of GC cells. miR-204 targeted Bcl-2 messenger RNA and increased responsiveness of GC cells to 5-fluorouracil and oxaliplatin treatment. Ectopic expression of Bcl-2 protein counteracted miR-204 pro-apoptotic activity in response to 5-fluorouracil. Altogether, these findings suggest that modulation of aberrant expression of miR-204, which in turn releases oncogenic Bcl-2 protein activity might hold promise for preventive and therapeutic strategies of GC.

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“…Sushma T.A., 5 found that out of 65 cases serum PSA values over 10ng/ml were seen in 10 benign (16.6%) cases and 3 malignant (60%) cases. V. Balachandar et al 6 in 2007,noted in his study that out of total 45 prostate cancer patients, PSA level of collected blood samples was ≤ 10.0 ng/ml in 39 subjects (86.7%) and ≥10.0 ng/ml in 6 subjects (13.3%). V. Balachandar et al 1 in 2008, found that range of PSA values in Prostate Cancer patients were 8 to 12 ng/ml.…”

Section: Discussionmentioning

confidence: 93%

“…The most frequent losses were on chromosomes 2, 5, 6, 8, 10, 13, 15, and 16. V. Balachandar et al, 6 in 2007, found that among 45 blood samples from prostate cancer patients, major chromosomal aberrations like deletion, translocation, inversion seen in chromosomes 1, 3, 5, 6, 7, 9, 13, 16, 18 and X and mosaic were identified in patients. V. Balachandar et al, 1 in 2008, studied 18 prostate cancer patients and found that chromosome 1, 2, 3 6, 7, 9, 16, 22, and X were the affected chromosome in Prostate Cancer patients.…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic Analysis of Prostate Carcinoma Patients

Chauhan

Damor

Solanki

et al. 2013

Int J of Biomed & Adv Res

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Background & objectives:

Prostate carcinoma is the second leading cause of cancer death in men above age of 60 years worldwide. More than 80% of the cancers concomitantly arise with Benign prostate hyperplasia. Prostate carcinoma is associated with various lower urinary tract symptoms, which affect their day to day life. The aim of the present investigation was to find out the major chromosomal aberrations present in Prostate carcinoma patients and to make a comparison with other study.

Methods:

In present study 05 cases of prostate carcinoma were taken on the basis of clinical diagnosis from the Gujarat Cancer Research Institute (GCRI), Ahmedabad, Gujarat, India, during the period of March 2011 to October 2011. The patients were analyzed for chromosomal aberrations using cultured peripheral blood lymphocytes with their pre-informed written consent.

Results:

In the present study, all 5 cases were > 60 years of age. 3(60%) cases of prostate carcinoma have elevated PSA level (>4 ng/ml). In adenocarcinoma of prostate gain, deletion, and translocation of 7q22-q31 is common. In present study, Karyotype analysis reveal 01 prostate carcinoma patient had 46,XY,del(7)(q31). In present study 2 cases shows evidence of metastasis.

Conclusion: Prostate carcinoma is found in > 60 years of age. Chromosome 1, 2, 3, 6, 7, 9, 16, 22, X and many more chromosomes were affected in prostate carcinoma patients, Common chromosomal aberration is 46,XY,del(7)(q31). Patients with prostate carcinoma have elevated PSA level (>4 ng/ml). Prostate carcinoma associated with metastasis have poor prognosis.

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Identification of a high frequency of chromosomal rearrangements in the centromeric regions of prostate cancer patients

Cited by 8 publications

References 42 publications

KDM2A represses transcription of centromeric satellite repeats and maintains the heterochromatic state

KDM2A represses transcription of centromeric satellite repeats and maintains the heterochromatic state

miR-204 targets Bcl-2 expression and enhances responsiveness of gastric cancer

Cytogenetic Analysis of Prostate Carcinoma Patients

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