Identification of a miRNA signature in neutrophils after traumatic injury

Abstract

Traumatic injury is the cause of significant mortality and morbidity. The molecular mechanisms underlying traumatic injury logically involve changes in gene expression that may be regulated through microRNAs (miRNAs). However, the association between miRNA deregulation and traumatic injury is largely unknown. The purpose of the present study is to address this issue. In this study, we used microarray profiling to evaluate the differential expressions of miRNAs in neutrophils obtained from patients with major trauma (injury severity scores >16), relative to healthy individuals. This neutrophilic miRNA signature was further validated using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). Genes and signaling pathways related to trauma-induced deregulated miRNAs were investigated in silico using the ontology-based and network mapping algorithms of Gene Ontology and Kyoto Encyclopedia of Genes or Genomes. Results showed that 13 miRNAs in neutrophils of major trauma patients were significantly and differentially expressed compared with the miRNA profiles of healthy controls. The results of qRT-PCR and in silico analysis revealed that miR-23a-5p, miR-30e-3p, miR-223-5p, miR-3945, miR-155-5p, and miR-150-5p were likely participants in the traumatic pathogenesis of these patients. In conclusion, neutrophils associated with traumatic injury were found to have a unique miRNA signature. Changes in signaling pathways due to deregulated miRNAs may be involved in the pathological processes of traumatic injury.

Overexpression of miR-1260b in Non-small Cell Lung Cancer is Associated with Lymph Node Metastasis

“…It could also be a signature to differentiate hereditary and non-hereditary breast cancers [31]. The miR-23a-5p was only identified as a participant in the pathogenesis of traumatic injury [32]. No study has explored the association of miR-1260b expression and metastasis.…”

The impact of trauma on neutrophil function

“…Examples of these include the recent description of trauma-induced changes in the composition of the circulating neutrophil pool [16] and the observation that trauma serves as a stimulus for the generation of neutrophil extracellular traps [17]. Alongside these findings, it has become apparent through the use of proteomics and microRNA profiling that trauma has a significant impact upon neutrophil signalling [18][19][20]. In this section, we review the findings of the most recent studies to have investigated trauma-induced changes in neutrophil biology.…”

“…To ascertain whether alterations to signalling pathways may underlie trauma-induced changes in neutrophil biology, comparative proteomics and microRNA (miRNA) profiling have been performed on neutrophils isolated from healthy controls and injured subjects [18][19][20]. MicroRNAs (miRNAs) are evolutionary conserved, small non-coding RNA molecules that assist in post transcriptional regulation [55].…”

Specific miRNA and its target in neutrophils after traumatic injury

“…Previously, we identified the miRNA profiles in neutrophils from major trauma patients and normal controls via miRNA microarray analysis and quantitative real-time PCR (qRT-PCR). Our results showed that the expression of miR-3945, miR-125a-5p, miR-363-3p, and miR-150-5p was significantly altered in neutrophils of patients suffering from trauma [12].…”

“…Fortunately, miRNA profiling can help to provide a better understanding of the physiological and pathological processes of traumatic injury [10,21,22]. Previously, we reported that miR-3945, miR-125a-5p, miR-363-3p, and miR-150-5p were significantly altered in neutrophils of patients suffering from major trauma [12]. However, pathological conditions such as inflammatory diseases may also affect neutrophil count and physiology, because these cells are the first line of defense against invading pathogens [23,24].…”