Identification of a new genotype of bovine leukemia virus

Balić, Davor; Lojkić, Ivana; Periškić, Marin; Bedeković, Tomislav; Jungić, Andreja; Lemo, Nina; Roić, Besi; Čač, Željko; Barbić, Ljubo; Madić, Josip

doi:10.1007/s00705-012-1300-4

Cited by 41 publications

(44 citation statements)

References 35 publications

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“…2. The results were similar to those of previous studies where BLV strains were divided into eight genotypes [27,30,31] and an extra Iranian isolate cluster [36]. Interestingly, the 43 Philippine BLV strains clustered either with genotype 1 or genotype 6.…”

Section: Detection Of Blv Infection By Blv-cocomo-qpcr-2 and Nested Pcrsupporting

confidence: 90%

“…Therefore, to gain insight into the degree of genetic variability of BLV strains in the Philippines, 43 field strains, which were representative of different breeds on the BLV-infected farms, were selected for phylogenetic characterization. After direct sequencing, 423-bp sequences corresponding to nucleotide positions 5126 to 5548 of the full-length BLV genome (BLV isolate LS3, GenBank accession number HE 967303) were aligned with 72 corresponding sequences from known BLV strains representing all BLV genotypes, including genotypes 1-8 [27,[29][30][31]. A neighbor-joining phylogenetic tree was then constructed using the TamuraNei model of nucleotide substitution [35].…”

Section: Detection Of Blv Infection By Blv-cocomo-qpcr-2 and Nested Pcrmentioning

confidence: 99%

“…Analysis of BLV gp51 env gene sequences from different geographic regions has revealed the presence of different genetic groups that correlate with the geographic origin of the isolates [21][22][23][24][25][26][27][28][29]. Recent phylogenetic studies on the BLV env gene of strains isolated worldwide demonstrate that the virus can be classified into eight genotypes [27,30,31].…”

Section: Introductionmentioning

confidence: 99%

“…Recent phylogenetic studies on the BLV env gene of strains isolated worldwide demonstrate that the virus can be classified into eight genotypes [27,30,31]. However, there are few studies on the distribution of BLV infections on Philippine farms, and until now, no study has focused on the genetic variability of BLV in the Philippines.…”

Section: Introductionmentioning

confidence: 99%

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Detection and molecular characterization of bovine leukemia virus in Philippine cattle

et al. 2014

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Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, which is the most common neoplastic disease of cattle. BLV infects cattle worldwide, imposing a severe economic impact on the dairy cattle industry. However, there are no comprehensive studies on the distribution of BLV in the Philippines, and the genetic characteristics of Philippine BLV strains are unknown. Therefore, the aim of this study was to detect BLV infections in the Philippines and determined their genetic variability. Blood samples were obtained from 1116 cattle from different farms on five Philippine islands, and BLV provirus was detected by BLV-CoCoMo-qPCR-2 and nested PCR targeting BLV long terminal repeats. Out of 1116 samples, 108 (9.7 %) and 54 (4.8 %) were positive for BLV provirus, as determined by BLV-CoCoMo-qPCR-2 and nested PCR, respectively. Of the five islands, Luzon Island showed the highest prevalence of BLV infection (23.1 %). Partial env gp51 genes from 43 samples, which were positive for BLV provirus by both methods, were sequenced for phylogenetic analysis. Phylogenetic analysis based on a 423-bp fragment of the env gene revealed that Philippine BLV strains clustered into either genotype 1 or genotype 6. Substitutions were mainly found in antigenic determinants, such as the CD4(+) T-cell epitope, the CD8(+) T-cell epitope, the second neutralizing domain, B and E epitopes, and these substitutions varied according to genotype. This study provides comprehensive information regarding BLV infection levels in the Philippines and documents the presence of two BLV genotypes, genotypes 1 and 6, in this population.

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Section: Detection Of Blv Infection By Blv-cocomo-qpcr-2 and Nested Pcrsupporting

confidence: 90%

Section: Detection Of Blv Infection By Blv-cocomo-qpcr-2 and Nested Pcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection and molecular characterization of bovine leukemia virus in Philippine cattle

et al. 2014

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“…The population of these viruses indicates a great genetic variability (3,5,19). This retroviral genetic variation is the composite of three variables: the mutation rate per replication cycle, the number of replication cycles, and the selective advantage or disadvantage possessed by variants of the viruses (4,11,24,25,27). Retroviral envelope (Env) proteins play multiple functions that are critical for viral replication and pathogenicity.…”

Section: Introductionmentioning

confidence: 99%

Flow Cytometric Determination of the Expression of gp 51 Protein of Bovine Leukaemia Virus in Experimentally Infected Sheep

Szczotka

2014

Bulletin of the Veterinary Institute in Pulawy

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The study was performed on lambs experimentally infected with bovine leukaemia virus (BLV). The presence of BLV antibodies in sera of infected animals was detected by agar gel immunodiffusion test and ELISA. Proviral DNA was detected by PCR and nested PCR. Dual-colour flow cytometry analysis was performed with the use of specific monoclonal antibodies against lymphocyte CD markers and gp51 viral envelope protein, followed by incubation with fluorescent-labelled secondary antibodies conjugated with FITC or PE. Gp51 viral envelope protein was detected in tumours caused by BLV infection. The BLV infection resulted in depletion of CD4+ lymphocytes, increase in CD8+ lymphocytes, and decrease in CD4+ to CD8+ ratio in infected sheep. Proliferation of IgM+ CD19+ cells was also detected. These cells had an immature character without tendency to differentiate, and their vitality was prolonged. Flow cytometry enabled detection of gp51 expression in sheep blood lymphocytes at the early stages of the infection, before detection of serum antibodies using ELISA.

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Detection of bovine leukemia virus and identification of its genotype in Mongolian cattle

et al. 2015

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Epidemiological studies have indicated that bovine leukemia virus (BLV) infection is globally distributed. However, no information regarding the disease and genetic diversity of the virus in the cattle of Mongolia is currently available. In this study, the prevalence of BLV was assessed using PCR, and the genetic diversity was analyzed through DNA sequencing. Of the 517 samples tested, 20 positives were identified. Phylogenetic analysis showed that six, one, and four isolates were classified into genotype 4, 7, and 1, respectively. Most isolates were clustered with isolates from Eastern Europe and Russia. This study is the first to investigate the BLV genotype in Mongolia.

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Identification of a new genotype of bovine leukemia virus

Cited by 41 publications

References 35 publications

Detection and molecular characterization of bovine leukemia virus in Philippine cattle

Detection and molecular characterization of bovine leukemia virus in Philippine cattle

Flow Cytometric Determination of the Expression of gp 51 Protein of Bovine Leukaemia Virus in Experimentally Infected Sheep

Detection of bovine leukemia virus and identification of its genotype in Mongolian cattle

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