Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion

Li, Ci-Xiu; Chang, Wei-Shan; Mitsakos, Katerina; Rodger, James A.; Holmes, Edward C.; Hudson, Bernard

doi:10.3390/v11080713

Cited by 17 publications

(23 citation statements)

References 20 publications

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“…Of particular note has been the metagenomic discovery of many novel and highly diverse species of circular single stranded (CRESS) DNA viruses [9,[12][13][14][15]. Although total shotgun RNA sequencing, or meta-transcriptomics [5,16], has less often been used for DNA virus discovery [17], it is viable as long as the viruses in question are expressing RNA. While the reliance on active transcription means that some DNA viruses will necessarily be missed, meta-transcriptomics provides an in-built indication of the abundance of viral reads in the data and has the ability to explore the whole virome within a specific host, identifying those that are highly divergent or at low abundance.…”

Section: Introductionmentioning

confidence: 99%

Diversity and Evolution of Novel Invertebrate DNA Viruses Revealed by Meta-Transcriptomics

Porter

Shī

Eden

et al. 2019

Viruses

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DNA viruses comprise a wide array of genome structures and infect diverse host species. To date, most studies of DNA viruses have focused on those with the strongest disease associations. Accordingly, there has been a marked lack of sampling of DNA viruses from invertebrates. Bulk RNA sequencing has resulted in the discovery of a myriad of novel RNA viruses, and herein we used this methodology to identify actively transcribing DNA viruses in meta-transcriptomic libraries of diverse invertebrate species. Our analysis revealed high levels of phylogenetic diversity in DNA viruses, including 13 species from the Parvoviridae, Circoviridae, and Genomoviridae families of single-stranded DNA virus families, and six double-stranded DNA virus species from the Nudiviridae, Polyomaviridae, and Herpesviridae, for which few invertebrate viruses have been identified to date. By incorporating the sequence of a “blank” experimental control we also highlight the importance of reagent contamination in metagenomic studies. In sum, this work expands our knowledge of the diversity and evolution of DNA viruses and illustrates the utility of meta-transcriptomic data in identifying organisms with DNA genomes.

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Section: Introductionmentioning

confidence: 99%

Diversity and Evolution of Novel Invertebrate DNA Viruses Revealed by Meta-Transcriptomics

Porter

Shī

Eden

et al. 2019

Viruses

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“…PVs were previously suspected to be involved in genital papillomas and carcinomas and aural plaques, but EcPV2 and EcPV3, respectively, were not fully characterised from samples of these lesions until 2011 (Lange et al 2011). EcPVs 4 to 7 were first reported in 2013 (Lange et al 2013), EcPV8 in 2018 (Linder et al 2018) and EcPV9 in 2019 (Li et al 2019). In 2013, BPV13 was linked with sarcoids after being detected in samples in Brazil (Lunardi et al 2013).…”

Section: Progress In Papillomavirus Discoverymentioning

confidence: 99%

Papillomaviruses in equids: A decade of discovery and more to come?

Jones

2021

Equine Veterinary Education

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“…The viruses infect different animals including humans, horses and bovine [1]. Till now nine species of EcPV, that are capable of infecting horses, have been documented [2,[4][5][6][7], however, there is no information on EcPV infection in horses within China.…”

Section: Introductionmentioning

confidence: 99%

First Detection and Genetic Characterization of Equus Caballus Papillomavirus 1, 2 and 7 in China

Tong¹,

Song²,

Ren³

et al. 2021

Preprint

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Background: Nine species of Equus caballus papillomavirus (EcPV) have been reported to infect horses, however, there are so far no reports of such infections in China. Results: In our pioneer study with Chinese horses, we found EcPV-1 in intranasal papilloma and nasal swabs, EcPV-2 in nasal swabs and semen, and EcPV-7 primarily in semen. This indicates that EcPVs are indeed hosted by horses in China, and that EcPV-2 and 7 may be getting transmitted though breeding. Sequence analyses for complete genomic sequences of EcPV-1 (G2), EcPV-2 (XJ-KS1391) and EcPV-7 (XJ-zs1) were performed which indicated that EcPV-1, 2 and 7, that infect horses in China, share 99.3% nt identity with the already published sequences for EcPV-1, 2 and 7. These observations indicate that three types of EcPVs identified in the current study are highly similar variants of previously known types of EcPV-1, 2 and 7. Phylogenetic analysis based on L1 genes in GenBank showed that EcPV-1, 2 and 7, found in Chinese horses, are closely related to and clustered together with already known EcPV-1, 2 and 7, respectively. Conclusion: Our study provides a novel evidence for EcPVs infection and circulation in Chinese horses and thus lays the foundation for a systematic and detailed epidemiological study of these infections in Chinese horses.

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Identification of a Novel Equine Papillomavirus in Semen from a Thoroughbred Stallion with a Penile Lesion

Cited by 17 publications

References 20 publications

Diversity and Evolution of Novel Invertebrate DNA Viruses Revealed by Meta-Transcriptomics

Diversity and Evolution of Novel Invertebrate DNA Viruses Revealed by Meta-Transcriptomics

Papillomaviruses in equids: A decade of discovery and more to come?

First Detection and Genetic Characterization of Equus Caballus Papillomavirus 1, 2 and 7 in China

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