Identification of a novel HLA‐B46 allele, B*4602, in Japanese

Abstract

The B*4602 was identified in a healthy Japanese donor. The B46 variant antigen differed serologically from B46. The nucleotide sequence of B*4602 differed from that of B*4601 by a single base (from A to G) at position 293 in exon 2. The substitution changed the amino acid Asp at position 74 to Gly. The gene frequency of B*4602 in the Japanese population is extremely low. The presumed haplotype of B*4602 was HLA-A2-Cw1-B*4602-DRB1*09. This is the first report of polymorphism of the B46 antigen.

Heat shock cognate protein 70 encodes antigenic epitopes recognised by HLA-B4601-restricted cytotoxic T lymphocytes from cancer patients

“…The HLA-class I of PBMCs was serotyped by conventional serological methods, as reported previously (Yang et al, 1999). The HLA-B alleles were also genotyped by the methods as reported previously (Azuma et al, 2003), and all of them were B4601, which was expected since the genotype of B46 in Asians is predominantly (495%) a B4601 (Imanishi et al, 1992;Barber et al, 1996;Akesaka et al, 2000). For the induction of peptide-specific CTLs, PBMCs (1 Â 10 5 cells per well) were incubated with 10 mM of each peptide in a round-bottom 96-well microculture plate (Nunc, Roskilde, Denmark) in 200 ml culture medium containing IL-2, as reported previously (Maeda et al, 2002).…”