Identification of a novel <i>Arabidopsis thaliana</i>
 nitric oxide-binding molecule with guanylate cyclase activity <i>in vitro</i>

Mulaudzi, Takalani; Ludidi, Ndiko; Ruzvidzo, Oziniel; Morse, Monique; Hendricks, Nicolette R.; Iwuoha, Emmanuel I.; Gehring, Chris

doi:10.1016/j.febslet.2011.07.023

Cited by 81 publications

(68 citation statements)

References 24 publications

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“…This result is in agreement with the mechanism for the metabolic reaction of cytochrome P450 (heamolytic) enzymes [21][22][23]. Figure 6 is the plot of the square wave voltammograms (SWVs) of the biosensor responses to PZA.…”

Section: Fig 3 Afm Images Of Mwcnts (A) Pani (B) and Pani-mwcnt Cosupporting

confidence: 73%

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Polyanilino-Carbon Nanotubes Derivatised Cytochrome P450 2E1 Nanobiosensor for the Determination of Pyrazinamide Anti-Tuberculosis Drugs

Sidwaba

Ajayi

Feleni

et al. 2014

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Abstract. Pyrazinamine (PZA) is one of the most commonly prescribed anti-tuberculosis (anti-TB) drug due to its ability to significantly shorten the TB treatment period. However, excess PZA in the body causes hepatotoxicity and liver damage. This, therefore, calls for new methods for ensuring reliable dosing of the drug, which will differ from person to person due to interindividual differences in drug metabolism. A novel biosensor system for monitoring the metabolism of PZA was prepared with nanocomposite of multi-walled carbon nanotubes (MWCNTs), polyaniline (PANI) and cytochrome P450 3A4 (CYP3A4) electrochemically deposited on a glassy carbon electrode (GCE).The nanocomposite biosensor system exhibited enhanced electroactivity that is attributable to the catalytic effect of the incorporated MWCNTs. The biosensor had a sensitivity of 7.80 µA/µg mL -1 PZA and a dynamic linear range of 4.92 -160 ng/mL PZA.

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Section: Fig 3 Afm Images Of Mwcnts (A) Pani (B) and Pani-mwcnt Cosupporting

confidence: 73%

“…The increase in current is proportional to the amount of the analyte. There was also an observed anodic shift in the peak potential, which is attributed [21][22][23] ng/mL [24]. This falls within the DLR of the GCE/PANI/MWCNT/CYP2E1 nanobiosensor.…”

Section: Fig 3 Afm Images Of Mwcnts (A) Pani (B) and Pani-mwcnt Comentioning

confidence: 73%

Polyanilino-Carbon Nanotubes Derivatised Cytochrome P450 2E1 Nanobiosensor for the Determination of Pyrazinamide Anti-Tuberculosis Drugs

Sidwaba

Ajayi

Feleni

et al. 2014

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“…However, MYB44 also negatively regulates ABA responses (Jaradat et al, 2013;Li et al, 2014) involved in stomatal closure, leaf senescence, and ROS scavenging (Persak and Pitzschke, 2014). In our study, MYB44 expression correlated positively with glycerol levels and negatively with the expression of NOGC1, a nitric oxide-dependent guanylyl cyclase involved in stomatal closure via Ca 2+ signaling (Mulaudzi et al, 2011;Joudoi et al, 2013). In addition, MYB44 correlated positively (r = 0.85, P = 0.001) with the chloroplastic lipid transfer protein LTPc1 (At2g10940), which together with LTPc2 (At2g45180) was up-regulated during O 3 stress but not herbivore stress (Supplemental Figs.…”

Section: Effects Of O 3 Stress On Photosynthesis and Stomatal Regulationmentioning

confidence: 53%

Central Metabolic Responses to Ozone and Herbivory Affect Photosynthesis and Stomatal Closure

et al. 2016

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Plants have evolved adaptive mechanisms that allow them to tolerate a continuous range of abiotic and biotic stressors. Tropospheric ozone (O 3 ), a global anthropogenic pollutant, directly affects living organisms and ecosystems, including plant-herbivore interactions. In this study, we investigate the stress responses of Brassica nigra (wild black mustard) exposed consecutively to O 3 and the specialist herbivore Pieris brassicae. Transcriptomics and metabolomics data were evaluated using multivariate, correlation, and network analyses for the O 3 and herbivory responses. O 3 stress symptoms resembled those of senescence and phosphate starvation, while a sequential shift from O 3 to herbivory induced characteristic plant defense responses, including a decrease in central metabolism, induction of the jasmonic acid/ethylene pathways, and emission of volatiles. Omics network and pathway analyses predicted a link between glycerol and central energy metabolism that influences the osmotic stress response and stomatal closure. Further physiological measurements confirmed that while O 3 stress inhibited photosynthesis and carbon assimilation, sequential herbivory counteracted the initial responses induced by O 3 , resulting in a phenotype similar to that observed after herbivory alone. This study clarifies the consequences of multiple stress interactions on a plant metabolic system and also illustrates how omics data can be integrated to generate new hypotheses in ecology and plant physiology.

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“…The cell-permeating analog of cGMP, 8-bromoguanosine 39,59-cyclic monophosphate (8-bromo-cGMP), induced expression of genes encoding PATHOGEN-RELATED1 protein, Phe ammonia-lyase, cation transporters, and chalcone synthase (Bowler et al, 1994;Durner et al, 1998;Maathuis, 2006;Suita et al, 2009). Recent work identified a novel plant NO-sensitive guanylate cyclase (Mulaudzi et al, 2011). Thus, a NO/cGMP signaling cascade resembling that in animal cells also appears to operate in plants.…”

Section: Introductionmentioning

confidence: 99%

Nitrated Cyclic GMP Modulates Guard Cell Signaling inArabidopsis

et al. 2013

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Nitric oxide (NO) is a ubiquitous signaling molecule involved in diverse physiological processes, including plant senescence and stomatal closure. The NO and cyclic GMP (cGMP) cascade is the main NO signaling pathway in animals, but whether this pathway operates in plant cells, and the mechanisms of its action, remain unclear. Here, we assessed the possibility that the nitrated cGMP derivative 8-nitro-cGMP functions in guard cell signaling. Mass spectrometry and immunocytochemical analyses showed that abscisic acid and NO induced the synthesis of 8-nitro-cGMP in guard cells in the presence of reactive oxygen species. 8-Nitro-cGMP triggered stomatal closure, but 8-bromoguanosine 39,59-cyclic monophosphate (8-bromocGMP), a membrane-permeating analog of cGMP, did not. However, in the dark, 8-bromo-cGMP induced stomatal opening but 8-nitro-cGMP did not. Thus, cGMP and its nitrated derivative play different roles in the signaling pathways that lead to stomatal opening and closure. Moreover, inhibitor and genetic studies showed that calcium, cyclic adenosine-59-diphosphate-ribose, and SLOW ANION CHANNEL1 act downstream of 8-nitro-cGMP. This study therefore demonstrates that 8-nitro-cGMP acts as a guard cell signaling molecule and that a NO/8-nitro-cGMP signaling cascade operates in guard cells.

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Identification of a novel Arabidopsis thaliana nitric oxide-binding molecule with guanylate cyclase activity in vitro

Cited by 81 publications

References 24 publications

Polyanilino-Carbon Nanotubes Derivatised Cytochrome P450 2E1 Nanobiosensor for the Determination of Pyrazinamide Anti-Tuberculosis Drugs

Polyanilino-Carbon Nanotubes Derivatised Cytochrome P450 2E1 Nanobiosensor for the Determination of Pyrazinamide Anti-Tuberculosis Drugs

Central Metabolic Responses to Ozone and Herbivory Affect Photosynthesis and Stomatal Closure

Nitrated Cyclic GMP Modulates Guard Cell Signaling inArabidopsis

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