Identification of a novel insect neuropeptide, CNMa and its receptor

Jung, Sung-Ho; Lee, Jae-hyuk; Chae, Hyo Seok; Seong, Jae Young; Park, Yoonseong; Park, Zee Yong; Kim, Young-Joon

doi:10.1016/j.febslet.2014.04.028

Cited by 45 publications

(31 citation statements)

References 14 publications

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“…The number of transcriptome reads specific for the first transcript is much smaller (75) than that for the second (931) and it thus seems plausible that the second peptide is produced in larger amounts than the first. As in several Hymenoptera and Nilaparvata (Jung et al, 2014 ; Tanaka et al, 2014 ) the termite genome also contains two CNMamide receptor homologs that on phylogenetic trees cluster tightly with the de-orphanized CNMamide receptors and hence are most likely the Zootermopsis receptors for these neuropeptides (Supplementary Figure 3 ). Only a single CNMa peptide was found encoded by the orthologous Locusta gene.…”

Section: Resultsmentioning

confidence: 94%

“…The data for Zootermopsis allowed us to predict complete precursor sequences for virtually all known insect neuropeptides, including such recent additions as the CCHamides, ACP, RYamide, trissin, natalisin, and CNMa (Roller et al, 2008 ; Hansen et al, 2010 ; Hauser et al, 2010 ; Ida et al, 2011a , b , 2012 ; Jiang et al, 2013 ; Jung et al, 2014 ). In a number of cases, exons were lacking either completely or partially from the genome assemblies, but all these gaps could be filled and/or corrected with transcriptome data and in a few cases those corrections were confirmed with the genomic reads.…”

Section: Resultsmentioning

confidence: 99%

“…CNMa was very recently identified as a novel insect neuropeptide (Jung et al, 2014 ). The Zootermopsis CNMa gene produces by alternative splicing two types of mRNA that differ by the inclusion or exclusion of the third coding exon (Figure 6 ).…”

Section: Resultsmentioning

confidence: 99%

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The contribution of the genomes of a termite and a locust to our understanding of insect neuropeptides and neurohormones

2014

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The genomes of the migratory locust Locusta migratoria and the termite Zootermopsis nevadensis were mined for the presence of genes encoding neuropeptides, neurohormones, and their G-protein coupled receptors (GPCRs). Both species have retained a larger number of neuropeptide and neuropeptide GPCRs than the better known holometabolous insect species, while other genes that in holometabolous species appear to have a single transcript produce two different precursors in the locust, the termite or both. Thus, the recently discovered CNMa neuropeptide gene has two transcripts predicted to produce two structurally different CNMa peptides in the termite, while the locust produces two different myosuppressin peptides in the same fashion. Both these species also have a calcitonin gene, which is different from the gene encoding the calcitonin-like insect diuretic hormone. This gene produces two types of calcitonins, calcitonins A and B. It is also present in Lepidoptera and Coleoptera and some Diptera, but absent from mosquitoes and Drosophila. However, in holometabolous insect species, only the B transcript is produced. Their putative receptors were also identified. In contrast, Locusta has a highly unusual gene that codes for a salivation stimulatory peptide. The Locusta genes for neuroparsin and vasopressin are particularly interesting. The neuroparsin gene produces five different transcripts, of which only one codes for the neurohormone identified from the corpora cardiaca. The other four transcripts code for neuroparsin-like proteins, which lack four amino acid residues, and that for that reason we called neoneuroparsins. The number of transcripts for the neoneuroparsins is about 200 times larger than the number of neuroparsin transcripts. The first exon and the putative promoter of the vasopressin genes, of which there are about seven copies in the genome, is very well-conserved, but the remainder of these genes is not. The relevance of these findings is discussed.

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Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

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The contribution of the genomes of a termite and a locust to our understanding of insect neuropeptides and neurohormones

2014

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“…We screened the assembled transcript libraries for the following neuropeptide-containing precursors: adipokinetic hormone/corazonin-related peptide (ACP), adipokinetic hormone (AKH), FGLamide allatostatin (AST-A), allatostatin C and CC (AST-C, AST-CC), allatotropin (AT), CAPA, crustacean cardioactive peptide (CCAP), CCHamide1 (CCHa1), CCHamide2 (CCHa2), corazonin, CNMamide (CNMa), corticotropin-releasing factor-related diuretic hormone (CRF-DH), calcitonin-related diuretic hormone (CT-DH), elevenin, ecdysis-triggering hormone (ETH), extended FMRFamide (FMRFa), inotocin, insect kinin, ion transport peptide (ITP), myoinhibitory peptide (MIP/AST-B), myosuppressin (MS), natalisin, neuropeptide F (NPF), neuropeptide-like precursor1 (NPLP1), orcokinin and orcomyotropin (orcokinin A, B), pigment-dispersing factor (PDF), proctolin, pyrokinin/pheromone biosynthesis activating neuropeptide (PK/PBAN), RYamide (RYa), SIFamide (SIFa), EFLamide (EFLa), sulfakinin (SK), short neuropeptide F (sNPF), tachykinin-related peptide (TKRP), and trissin. For most of the neuropeptides that we searched for in this study, the corresponding G-protein-coupled receptors are known from insects [ 15 – 17 ]. For the NPLP1 peptides, a membrane-bound guanylate cyclase has been described as a receptor in D. melanogaster [ 18 ].…”

Section: Resultsmentioning

confidence: 99%

Evolution of neuropeptides in non-pterygote hexapods

Derst¹,

Dircksen²,

Meusemann³

et al. 2016

BMC Evol Biol

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BackgroundNeuropeptides are key players in information transfer and act as important regulators of development, growth, metabolism, and reproduction within multi-cellular animal organisms (Metazoa). These short protein-like substances show a high degree of structural variability and are recognized as the most diverse group of messenger molecules. We used transcriptome sequences from the 1KITE (1K Insect Transcriptome Evolution) project to search for neuropeptide coding sequences in 24 species from the non-pterygote hexapod lineages Protura (coneheads), Collembola (springtails), Diplura (two-pronged bristletails), Archaeognatha (jumping bristletails), and Zygentoma (silverfish and firebrats), which are often referred to as “basal” hexapods. Phylogenetically, Protura, Collembola, Diplura, and Archaeognatha are currently placed between Remipedia and Pterygota (winged insects); Zygentoma is the sistergroup of Pterygota. The Remipedia are assumed to be among the closest relatives of all hexapods and belong to the crustaceans.ResultsWe identified neuropeptide precursor sequences within whole-body transcriptome data from these five hexapod groups and complemented this dataset with homologous sequences from three crustaceans (including Daphnia pulex), three myriapods, and the fruit fly Drosophila melanogaster. Our results indicate that the reported loss of several neuropeptide genes in a number of winged insects, particularly holometabolous insects, is a trend that has occurred within Pterygota. The neuropeptide precursor sequences of the non-pterygote hexapods show numerous amino acid substitutions, gene duplications, variants following alternative splicing, and numbers of paracopies. Nevertheless, most of these features fall within the range of variation known from pterygote insects. However, the capa/pyrokinin genes of non-pterygote hexapods provide an interesting example of rapid evolution, including duplication of a neuropeptide gene encoding different ligands.ConclusionsOur findings delineate a basic pattern of neuropeptide sequences that existed before lineage-specific developments occurred during the evolution of pterygote insects.Electronic supplementary materialThe online version of this article (doi:10.1186/s12862-016-0621-4) contains supplementary material, which is available to authorized users.

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“…The fruit fly Drosophila melanogaster genome has at least 45 neuropeptide genes, which encode much larger numbers of mature neuropeptides [6,13,17]. The number of genes encoding neuropeptides continues to increase as additional genes and mRNA species from animal genomes are uncovered thanks to advances in bioinformatical tools and RNA sequencing technology.…”

Section: Introductionmentioning

confidence: 99%