Identification of a RAPD Marker Linked to the Susceptible Gene of Black Spot Disease in Japanese Pear.

Banno, Kiyoshi; Ishikawa, Hironobu; Hamauzu, Yasunori; Tabira, Hiroki

doi:10.2503/jjshs.68.476

Cited by 27 publications

(6 citation statements)

References 12 publications

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“…In related studies on pears, RAPD markers have been used to identify parentage (Banno et al, 2000) and a marker linked to the gene conferring susceptibility to black spot disease [Alternaria alternata (Fr.) Keissler] (Banno et al, 1999). Oliveira et al (1999) and Monte-Corvo et al (2000) have confirmed that RAPD is useful in pear cultivar identification and phenetic classification within the genus Pyrus.…”

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confidence: 92%

Genetic Relationships of Pyrus Species and Cultivars Native to East Asia Revealed by Randomly Amplified Polymorphic DNA Markers

Teng¹,

Tanabe²,

Tamura³

et al. 2002

jashs

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A total of 118 Pyrus sp. (pear) and cultivars native mainly to east Asia were subjected to randomly amplified polymorphic DNA (RAPD) analysis to evaluate genetic variation and relationships among the accessions. Two hundred fifty RAPD markers were scored from 20 decamer primers. RAPD markers specific to species were identified. Clustering analysis revealed two divisions: one comprising cultivars of P. communis L., and the other including all accessions of Pyrus native to east Asia. The grouping of the species and cultivars by RAPD data largely agrees with morphological pear taxonomy. However, some noted incongruence existed between two classification methods. Pyrus calleryana Dcne. clustered together with P. koehnei Schneid., P. fauriei Schneid. and P. dimorphophylla Makino. Pyrus betulaefolia Bge. clustered with P. ×hopeiensis Yu and P. ×phaeocarpa Rehd. A noncultivated clone of P. aromatica Kikuchi et Nakai grouped with P. aromatica cultivars. Pyrus hondoensis Nakai et Kikuchi and cultivars of P. ussuriensis Max. formed a single group. Some accessions from Korea (named Korean pear) had species-specific RAPD markers and comprised an independent group. Most of the Chinese white pears clustered together with most of the Chinese sand pears. Based on the present results, the new nomenclature P. pyrifolia var. sinensis (Lindley) Teng et Tanabe for Chinese white pear was suggested. Most accessions of Japanese pears fell into one main group, whereas pear cultivars from Kochi Prefecture of Japan subclustered with some Chinese sand pears and one accession from Korea. Our results infer that some local Japanese pear cultivar populations may have been derived from cultivars native to Kochi Prefecture in Shikoku region, and that the latter may have been introduced from ancient China and/or Korea.

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confidence: 92%

Genetic Relationships of Pyrus Species and Cultivars Native to East Asia Revealed by Randomly Amplified Polymorphic DNA Markers

Teng¹,

Tanabe²,

Tamura³

et al. 2002

jashs

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“…However, it may take approximately 4 years to screen new seedless/seedy hybrids between aborted anther types and normal anther types by using this morphological marker. BSA allows widespread application of profile markers linked to genetic traits [3,23]. BSA was easy to construct two pooled DNA samples of individuals from two segregating populations (male sterility/male fertility) using 'Kiyomi' tangor as female parent.…”

Section: Resultsmentioning

confidence: 99%

Development of a SCAR Marker Linked to Male Fertility Traits in 'Jinkyool' (Citrus sunki)

Chae¹,

Yun²,

Park³

et al. 2011

Journal of Life Science

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In Citrus, an F1 segregation population of 150 plants was constructed from a cross between 'Kiyomi' (C. unshiu × C. sinensis) carrying the male sterility trait and 'Jinkyool' (C. sunki). Sequence-related amplification polymorphism (SRAP) combined with bulked segregant analysis was used to develop markers linked to male fertility. In the F1 population, 66 out of 150 seedlings had aborted anthers and the ratio of male sterile plants to fertile plants in the progenies matched the expected Mendelian segregation ratio of 1:1 (χ 2 =2.16 at p=0.05). From the profiling of the 197 SRAP primer sets, three SRAP primer sets (F4/R27, F39/R60, and F15/R37) that were closely linked to the target trait were identified and successfully converted into a sequence characterized amplified region (SCAR) marker for selection of male fertility in citrus. The SCAR marker, using the pMS 33U/pMS 1462L primer set specifically, produced a single 1.4-Kb fragment that was linked to male fertility. Our results suggested that this SCAR marker can be useful for marker-assisted selection of male sterile individuals in breeding F1 progenies in Citrus.

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“…The location of the gene(s) responsible for resistance (or susceptibility) to the most important fungal disease, black spot caused by the Alternaria alternata Japanese pear pathotype, has been identified (Banno et al, 1999;Iketani et al, 2001;Terakami et al, 2007). Terakami et al (2016) finely mapped the gene for susceptibility to black spot disease to the top region of LG 11 in the Japanese pear genome (Fig.…”

Section: Mas In Japanese Pear Breeding Programsmentioning

confidence: 99%

DNA Markers and Molecular Breeding in Pear and Other Rosaceae Fruit Trees

Yamamoto

2021

Hortic J

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Pear (Pyrus spp.) is one of the most important edible fruits belonging to the family Rosaceae. DNA markers, molecular genetics and genomics, and molecular breeding of pear have greatly progressed over the last few decades. The development of reliable DNA markers, such as simple sequence repeats and single nucleotide polymorphisms, has allowed DNA profiling of pear accessions, assessment of the genetic diversity within pear species, and analyses of phylogenetic relationships among pear species. Reference genetic linkage maps and genome-wide molecular markers have enabled practical marker-assisted selection for resistance to black spot and/or pear scab diseases, self-compatibility, harvest time, and fruit skin color in Japanese pear breeding programs. Molecular breeding has been shown to more than triple the selection efficiency of practical breeding compared with conventional breeding. Furthermore, breeding programs using two novel genomics-based approaches-genome-wide association studies and genomic selection-focusing on fruit quality and texture, and quantitative traits for breeding, are in progress. Co-linearity and functional synteny have been identified between pear and apple (Malus × domestica Borkh), and have been used to efficiently predict the function of a gene of interest and develop selection markers in related species.

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Identification of a RAPD Marker Linked to the Susceptible Gene of Black Spot Disease in Japanese Pear.

Cited by 27 publications

References 12 publications

Genetic Relationships of Pyrus Species and Cultivars Native to East Asia Revealed by Randomly Amplified Polymorphic DNA Markers

Genetic Relationships of Pyrus Species and Cultivars Native to East Asia Revealed by Randomly Amplified Polymorphic DNA Markers

Development of a SCAR Marker Linked to Male Fertility Traits in 'Jinkyool' (Citrus sunki)

DNA Markers and Molecular Breeding in Pear and Other Rosaceae Fruit Trees

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