Identification of a Rat Adrenal Cytochrome P450 Active in Polycyclic Hydrocarbon Metabolism as Rat CYP1B1

Bhattacharyya, KK; Brake, PB; Eltom, SE; Otto, SA; Jefcoate, C. R.

doi:10.1074/jbc.270.19.11595

Cited by 154 publications

(59 citation statements)

References 28 publications

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“…Previous studies have shown induction of CYP1B1 in a variety of organs in fish and mammals exposed to AHR agonists (e.g., Bhattacharyya et al, 1995;El-kady et al, 2004a;Willett et al, 2006). More recently, CYP1C1 was reported to be induced in many of the same organs we examined, in killifish exposed to benzo[a]pyrene (B[a]P) (Wang et al, 2006).…”

Section: Induced Expression Of Cyp1 Genes In Adult Zebrafishmentioning

confidence: 66%

Basal and 3,3′,4,4′,5-pentachlorobiphenyl-induced expression of cytochrome P450 1A, 1B and 1C genes in zebrafish

Jönsson

Orrego

Woodin

et al. 2007

Toxicology and Applied Pharmacology

135

143

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The cytochrome P4501C (CYP1C) gene subfamily was recently discovered in fish, and zebrafish (Danio rerio) CYP1C1 transcript has been cloned. Here we cloned the paralogous CYP1C2, showing that the amino acid sequence is 78% identical to CYP1C1, and examined gene structure and expression of CYP1A, CYP1B1, CYP1C1, and CYP1C2. Xenobiotic response elements were observed upstream of the coding regions in all four genes. Zebrafish adults and embryos were exposed (24 hours) to 100 nM 3,3',4,4',5-polychlorinated biphenyl (PCB126) or 20 ppm acetone and subsequently held in clean water for 24 hours (adults) or 48 hours (embryos). All adult organs examined (eye, gill, heart, liver, kidney, brain, gut, and gonads) and embryos showed basal expression of the four genes. CYP1A was most strongly expressed in liver, whereas CYP1B1, CYP1C1, and CYP1C2 were most strongly expressed in heart and eye. CYP1B1 and the CYP1C genes showed an expression pattern similar to one another and to mammalian CYP1B1. In embryos CYP1C1 and CYP1C2 tended to have a higher basal expression than CYP1A and CYP1B1. PCB126 induced CYP1A in all organs, and CYP1B1 and CYP1C1 in all organs except gonads, or gonads and brain, respectively. CYP1C2 induction was significant only in the liver. However, in embryos all four genes were induced strongly by PCB126. The results are consistent with CYP1C1 and CYP1C2, as well as CYP1A and CYP1B1, being regulated by the aryl hydrocarbon receptor. While CYP1A may have a protective role against AHR agonists in liver and gut, CYP1B1, CYP1C1, and CYP1C2 may also play endogenous roles in eye and heart and possibly other organs, as well as during development.

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Section: Induced Expression Of Cyp1 Genes In Adult Zebrafishmentioning

confidence: 66%

Basal and 3,3′,4,4′,5-pentachlorobiphenyl-induced expression of cytochrome P450 1A, 1B and 1C genes in zebrafish

Jönsson

Orrego

Woodin

et al. 2007

Toxicology and Applied Pharmacology

135

143

View full text Add to dashboard Cite

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“…This human protein, designated cytochrome P4501B1 (CYP1B1), has also been identified and cloned in tissues from mouse (8,9) and rat (10,11). The estimated size of the rodent CYP1B1 mRNA (5.2 kb) is nearly identical to that reported for the human CYP1B1 mRNA (5.1 kb), and each predicts a protein of 543 amino acids.…”

mentioning

confidence: 55%

Isolation and Characterization of the Human Cytochrome P450 CYP1B1 Gene

Tang

Stewart

et al. 1996

Journal of Biological Chemistry

204

102

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“…However, as a result of the poor expression of CYP1B1 in the liver (Bhattacharyya et al, 1995), apoprotein levels were difficult to detect, especially after incubation of slices for 24 hours during which cytochromes P450 decline (Hashemi et al, 2000), and was monitored at the mRNA level.When CYP1B1 mRNA levels were monitored, once again a rise was noted in liver slices exposed to all PAHs, with …”

Section: Rat Liver Studiesmentioning

confidence: 99%

“…The cytochrome P450 family responsible for the metabolism, including bioactivation, of PAHs is CYP1, in particular CYP1A1 and CYP1B1 (Shimada and Fujii-Kuriyama, 1995;Ioannides and Lewis, 2004). Both of these enzymes are constitutively poorly expressed in the liver, and are essentially extrahepatic enzymes (Guengerich, 1990;Bhattacharyya et al, 1995). CYP1, however, is probably the most inducible CYP family, at least in terms of induction observed, being up-regulated by planar compounds in the liver and extrahepatic tissues of animals and humans (Ioannides and Parke, 1990;Christou et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Up-regulation of CYP1A/B in rat lung and liver, and human liver precision-cut slices by a series of polycyclic aromatic hydrocarbons; association with the Ah locus and importance of molecular size

Pushparajah

Umachandran

Nazir

et al. 2008

Toxicology in Vitro

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Identification of a Rat Adrenal Cytochrome P450 Active in Polycyclic Hydrocarbon Metabolism as Rat CYP1B1

Cited by 154 publications

References 28 publications

Basal and 3,3′,4,4′,5-pentachlorobiphenyl-induced expression of cytochrome P450 1A, 1B and 1C genes in zebrafish

Basal and 3,3′,4,4′,5-pentachlorobiphenyl-induced expression of cytochrome P450 1A, 1B and 1C genes in zebrafish

Isolation and Characterization of the Human Cytochrome P450 CYP1B1 Gene

Up-regulation of CYP1A/B in rat lung and liver, and human liver precision-cut slices by a series of polycyclic aromatic hydrocarbons; association with the Ah locus and importance of molecular size

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