Identification of a retinoic acid-inducible gene I from grass carp (Ctenopharyngodon idella) and expression analysis in vivo and in vitro

Yang, Chunrong; Su, Jian; Huang, Teng; Zhang, Rongfang; Peng, Limin

doi:10.1016/j.fsi.2011.01.020

Cited by 62 publications

(49 citation statements)

References 31 publications

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“…This fact would suggest a high IFN or antiviral response in the sea bass gonad and a very low response in the brain, which could explain the low resistance of this fish species, but this needs to be confirmed at functional level. These results are in agreement with other studies in fish showing the upregulation of most of these genes after virus infection in several tissues or their antiviral function after cell line overexpression (Biacchesi et al, 2009;Chen et al, 2015;Feng et al, 2011;Rise et al, 2008Rise et al, , 2010Skjesol et al, 2011;Su et al, 2010;Sun et al, 2011;Xiang et al, 2011;Yang et al, 2011), and support the fact that the sequences identified in our study mediate the IFN activation cascade. In the case of tbk1, which is also activated by the TLR response, it is only upregulated in sea bass specimens infected with VNNV.…”

Section: Discussionsupporting

confidence: 94%

“…Similar upregulation has been documented in the brain of sea bass and Atlantic halibut exposed to VNNV (Dios et al, 2007;Rise et al, 2010), and this supports our data. Moreover, these sensors are also upregulated by several fish RNA viruses or polyI: C in several tissues of fish, such as spleen, head-kidney, liver or intestine, as well as in some fish cell lines, leading to an increase in the type I IFN-mediated antiviral response (Feng et al, 2011;Rise et al, 2008Rise et al, , 2010Skjesol et al, 2011;Su et al, 2010;Yang et al, 2011). Moreover, fish rig1 and mda5 transient overexpression leads to the induction of ifn expression and confers an antiviral state (Biacchesi et al, 2009;Sun et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…This family has three members: RIG-I (also known as DDX58), melanoma differentiation-associated gene 5 (MDA5, also known as IFIN1) and Laboratory of Genetics and Physiology 2 (LGP2, also known as DHX58). These sensors are upregulated by Viral hemorrhagic septicemia virus (VHSV), Spring viremia of carp virus, Grass carp reovirus (GCRV; Aquareovius C), Viral nervous necrosis virus (VNNV; members of the genus Betanodavirus) and Infectious pancreatic necrosis virus (IPNV), as well as by polyinosinic acid (polyI: C; a synthetic analogue of viral dsRNA), leading to an increase in the IFN-mediated antiviral response (Chen et al, 2015;Feng et al, 2011;Rise et al, 2008Rise et al, , 2010Skjesol et al, 2011;Su et al, 2010;Yang et al, 2011). However, further studies are needed to definitely define their roles in the antiviral response, and to identify and characterize their mediators in the molecular pathway leading to IFN activation.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the IFN pathway in the teleost fish gonad against vertically transmitted viral nervous necrosis virus

Valero

Morcillo

Meseguer

et al. 2015

Journal of General Virology

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One of the most powerful innate immune responses against viruses is mediated by type I IFN. In teleost fish, it is known that virus infection triggers the expression of ifn and many IFN-stimulated genes, but the viral RNA sensors and mediators leading to IFN production are scarcely known. Thus, we have searched for the presence of these genes in gilt-head sea bream (Sparus aurata) and European sea bass (Dicentrarchus labrax), and evaluated their expression after infection with viral nervous necrosis virus (VNNV) in the brain, the main viral target tissue, and the gonad, used to transmit the virus vertically. In sea bream, a fish species resistant to the VNNV strain used, we found an upregulation of the genes encoding MDA5 (melanoma differentiation-associated gene 5), TBK1 (TANK-binding kinase 1), IRF3 (IFN regulatory factor 3), IFN, Mx [myxovirus (influenza) resistance protein] and PKR (dsRNA-dependent protein kinase receptor) proteins in the brain, which were unaltered in the gonad and could favour the dissemination by gonad fluids or gametes. Strikingly, in European sea bass, a very susceptible species, we also identified, transcripts coding for LGP2 (Laboratory of Genetics and Physiology 2), MAVS (mitochondrial antiviral signalling), TRAF3 (TNF receptor-associated factor 3), TANK (TRAF family member-associated NFkB activator) and IRF7 (IFN regulatory factor 7), and found that all the genes analysed were upregulated in the gonad, but only mda5, lgp2, irf3, mx and pkr were upregulated in the brain. These findings supported the notion that the European sea bass brain innate immune response is unable to clear the virus and pointed to the importance of gonad immunity to control the dissemination of VNNV to the progeny -an aspect that is worth investigating in aquatic animals.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of the IFN pathway in the teleost fish gonad against vertically transmitted viral nervous necrosis virus

Valero

Morcillo

Meseguer

et al. 2015

Journal of General Virology

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“…17 Cells were maintained at 28 uC with 5% CO 2 in 6-well culture plates. The GCRV 097 strain, which belongs to the type II GCRV, was used for challenge experiments in CIK cells.…”

Section: Dynamic Localization Of Hmgbs Induced By Gcrv Challenge Yl Rmentioning

confidence: 99%

Dynamic localization and the associated translocation mechanism of HMGBs in response to GCRV challenge in CIK cells

Rao

Yang

et al. 2014

Cell Mol Immunol

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High-mobility group box (HMGB) proteins, a family of chromatin-associated nuclear proteins, play amazingly multifaceted roles in the immune system of mammals. Thus far, little is known about the nucleocytoplasmic distribution of HMGBs in teleosts. The present study systematically investigated the dynamic localization of all six HMGB proteins in Ctenopharyngodon idella kidney (CIK) cells. Under basal conditions, all HMGBs exclusively localized to the nucleus. Grass carp reovirus (GCRV), polyinosinic-polycytidylic (poly(I : C)) potassium salt and lipopolysaccharide (LPS) challenge evoked the nuclear export of HMGBs to various degrees: GCRV challenge induced the highest nuclear export of CiHMGB2b, and poly(I : C) and LPS evoked the highest nucleocytoplasmic shuttling of CiHMGB1b. Overall, the nucleocytoplasmic shuttling of CiHMGB2a and CiHMGB3b was rarely induced by these challenges. Dynamic imaging uncovered that the nucleocytoplasmic GCRV-induced relocation of CiHMGB2b occurred in cells undergoing karyotheca rupture, apoptosis or proliferation. Western blot analyses were used to examine HMGB-EGFP fusion proteins in whole cell lysates, cytosol, nuclear fractions and culture medium. Further investigation demonstrated the nuclear retention of N-terminal HMG-boxes and the nucleocytoplasmic distribution of the C-terminal acidic tails. Comparative analyses of the dynamic relocation of full-length, truncated or chimeric HMGBs confirmed that the intramolecular interaction between HMG-boxes and C-tail domains mediated the nucleocytoplasmic translocation of HMGBs. These results not only provide an overall understanding of the subcellular localization of HMGBs, but also reveal the induction mechanism of the nucleocytoplasmic translocation of HMGBs by GCRV challenge, which lays a foundation for further studies on the interactions among pathogens, HMGBs and pattern recognition receptors in the innate immunity of teleosts. Keywords: grass carp (Ctenopharyngodon idella); grass carp reovirus; HMGBs; innate immunity; subcellular localization INTRODUCTION High-mobility group box (HMGB) proteins are abundant non-histone chromatin components implicated in major DNA transactions. In mammals, there are four family members (HMGB1-4), of which HMGB1, 2 and 3 are characterized by two DNA-binding domains (HMG-box A and B) and a Cterminal acidic tail domain and HMGB4 possesses two HMG-boxes but lacks the acidic tail. 1 In some teleosts, such as fugu (Takifugu), medaka (Oryzias latipes), Tetraodon and stickleback, two paralogous genes were detected for HMGB1 and HMGB2 but not for HMGB3. 2 However, in zebrafish (Danio rerio), salmon (Oncorhynchus), carp (Cyprinus carpio) 2 and grass carp (Ctenopharyngodon idella), 3-5 two paralogs are present within each of the HMGB subfamilies (HMGB1,

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“…b-Actin was used as the reference gene for all amplifications. Primers for RIG-1 have been reported before [13], and other sequences of homologous genes (in supplement) for zebrafish were searched in the grass carp genomic bank using a BLAST search.…”

Section: Gcjam-a-knockdown and Expression Analysis Of The Interferon mentioning

confidence: 99%

Identification of a retinoic acid-inducible gene I from grass carp (Ctenopharyngodon idella) and expression analysis in vivo and in vitro

Cited by 62 publications

References 31 publications

Characterization of the IFN pathway in the teleost fish gonad against vertically transmitted viral nervous necrosis virus

Characterization of the IFN pathway in the teleost fish gonad against vertically transmitted viral nervous necrosis virus

Dynamic localization and the associated translocation mechanism of HMGBs in response to GCRV challenge in CIK cells

Cloning and preliminary functional studies of the JAM-A gene in grass carp (Ctenopharyngodon idellus)

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