Identification of a set of RFLP probes for subspecies differentiation in Oryza sativa L.

Qian, H.-R.; Zhuang, Jie‐Yun; Lin, Haiyan; Lü, Jun; Kangle, Zheng

doi:10.1007/bf00222026

Cited by 20 publications

(10 citation statements)

References 8 publications

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“…However, it adopts so many quantitative traits and is easy to be influenced by subjective experiences. Along with the rapid development of molecular marker technology since 1980, it has been used widely in rice classification (Qian et al, 1995;Qi et al, 2001;Chen et al, 2002;Wang et al, 2006) and phylogeny Nonnatus et al, 2001;Sun et al, 2001;Cai et al, 2006), which is simple, convenient, objective, with no influence by environments. However, the molecular marker method has not become an independent classification system, which depends on the conventional morphological marker method to make verification.…”

Section: Discussionmentioning

confidence: 99%

Effect of ecological environments on subspecies characteristics and economic traits in filial generations of cross between indica and japonica rice

Liu

Chunjie

et al. 2008

Front. Agric. China

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Two recombinant inbred lines (RILs) populations, RILSA derived from the cross between "Zhongyouzao8" (indica) and "Toyonihiki" (japonica) rice cultivars, and RILSB derived from the cross between "Qishanzhan" (indica) and "Akihikari" (japonica) rice cultivars, were grown in Liaoning and Sichuan Provinces, China to understand the effects of ecological environments on the subspecies characteristics and economic traits in filial generations of cross between indica and japonica. The results showed that both the subspecies characteristics and economic traits changed significantly. The effects of ecological environments on Cheng's index and six subspecies characteristics were different on the basis of populations or characteristics. The distribution of Cheng's index in RILSA was japonicalinous in Liaoning and Sichuan. The distribution of Cheng's index in RILSB approached to normal distribution in Liaoning, but it was japonicalinous in Sichuan. As a whole, the two populations were more japonicalious in Sichuan than in Liaoning. The panicle number, seed setting rate and per-thousand-grain weight were decreased significantly in Sichuan. The grain number per panicle showed no significant change. A significant positive correlation was found between Cheng's index and the economic traits, including six subspecies traits. It suggested that the reason that the filial generation of cross between indica and japonica in northern China showed japonicanous subspecies characteristics might be the artificial selection by breeders on the economic traits. In addition, indica-japonica differentiation and the relationship with ecological environments were discussed.

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Section: Discussionmentioning

confidence: 99%

Effect of ecological environments on subspecies characteristics and economic traits in filial generations of cross between indica and japonica rice

Liu

Chunjie

et al. 2008

Front. Agric. China

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“…Total DNA extraction, restriction endonuclease digestion, electrophoresis, Southern blotting, hybridization and autoradiography were carried out following the methods described previously by Zheng et al (1990) and Lu and Zheng (1992). Thirty rice clones were selected based on our previous work on phylogenetic analysis of the cultivated O. sativa (Zheng et al 1994;Qian et al 1995), which are distributed over different chromosomes of the cultivated rice (see Table 2). Most clones (RG clones) are random genomic clones from Cornell University.…”

Section: Dna Extraction and Rflp Analysismentioning

confidence: 99%

Genetic differentiation of wild relatives of rice as assessed by RFLP analysis

et al. 2002

Theor Appl Genet

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To study genetic diversity and relationships of wild relatives of rice, 58 accessions of Oryza rufipogon, Oryza nivara, Oryza sativa f. spontanea and the cultivated Oryza sativa, representing a wide range of their distribution, were analyzed using the restriction fragment length polymorphism (RFLP) technique. All 30-used RFLP probes detected polymorphisms among the Oryza accessions, with an average of 3.8 polymorphic fragments per probe. Considerable genetic diversity was scored among the Oryza accessions with a similarity coefficient ranging from 0.28 to 0.93; but the cluster analysis of the accessions did not show an apparent grouping based on the species classification, instead they were scattered randomly in different groups. Noticeably, the Oryza accessions from the same geographic region, or near-by geographic regions, tended to be clustered in the same groups. The indica rice varieties showed relatively high genetic diversity and were scattered in different groups of their wild relatives, but the japonica varieties showed a relatively low variation and formed an independent group. It is concluded from the molecular analytical result that: (1) the four Oryza taxa have a remarkably close relationship and their independent species status need to be carefully reviewed; (2) geographic isolation has played a significant role in the differentiation of the Oryza accessions; therefore, a wide geographic range needs to be covered in collecting wild rice germplasm for ex situ conservation; and (3) the conventional conclusion of indica rice being directly domesticated from its ancestral wild species, and japonica rice being derived from indica, gains support from our data.

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“…These cultivars were characterized by RFLP markers. RFLP markers are useful for the classification of rice and several individual RFLP markers can be used to classify rices into indica and japonica, ecogeographical races (Zheng et al, 1994;Qian et al, 1995;Li et al, 1998). The RFLP markers diagnostic for the cultivar groups were screened from the markers on the rice RFLP genetic linkage map (Kurata et al, 1994), using a core collection of 130 rice cultivars whose cultivar groups had been identified (data are not shown).…”

Section: Methodsmentioning

confidence: 99%

Geographical variation of the genes controlling hybrid breakdown and genetic differentiation of the chromosomal regions harboring these genes in Asian cultivated rice, Oryza sativa L.

1998

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The geographical variation of two genes, hwd1 and hwd2, controlling hybrid breakdown (F 2 weakness) was surveyed in 239 Asian rice cultivars. The cultivars with the genotype Hwd1/Hwd1 Hwd2/Hwd2 were predominant in South Asia and Southeast Asia, while those with the genotype Hwd1/Hwd1 hwd2/hwd2 were predominant in Japan, the Philippines, and Yunnan province, China. Only one cultivar from Laos had the genotype hwd1/hwd1 Hwd2/Hwd2. Preferential association was found between the presence or absence of hwd2 and the two major cultivar groups (indica and japonica) based on diagnostic RFLP markers. Dominant homozygotes were frequent in indica rice cultivars, while recessive homozygotes were frequent in japonica rice cultivars. This association was not clear for cultivars from the center of genetic diversity of rice, ranging from Vietnam to Bangladesh. Polymorphisms were surveyed for RFLP markers on chromosomes 10 and 7, harboring hwd1 and hwd2 loci, respectively. The region around hwd2 tended to be differentiated in allelic combination among cultivars into the indica and japonica rice groups. No such trend was found around the hwd1 region. These facts suggest the hybrid breakdown controlled by this genic system is a rare phenomenon and consequently did not contribute to cultivar differentiation of rice.

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Identification of a set of RFLP probes for subspecies differentiation in Oryza sativa L.

Cited by 20 publications

References 8 publications

Effect of ecological environments on subspecies characteristics and economic traits in filial generations of cross between indica and japonica rice

Effect of ecological environments on subspecies characteristics and economic traits in filial generations of cross between indica and japonica rice

Genetic differentiation of wild relatives of rice as assessed by RFLP analysis

Geographical variation of the genes controlling hybrid breakdown and genetic differentiation of the chromosomal regions harboring these genes in Asian cultivated rice, Oryza sativa L.

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