2012
DOI: 10.1007/s00705-012-1224-z
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Identification of a small-molecule inhibitor of dengue virus using a replicon system

Abstract: Dengue virus (DENV) is a mosquito-borne human pathogen that causes a serious public-health threat in tropical and subtropical regions of the world. Neither a vaccine to prevent nor an effective therapeutic agent to treat DENV infection is currently available. We established a stable cell line harboring a luciferase-reporting DENV subgenomic replicon to screen for inhibitors of DENV. A total of 14,400 small-molecule (MW < 500 Da) chemicals were evaluated for their ability to reduce luciferase reporter activity … Show more

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Cited by 28 publications
(32 citation statements)
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“…DENV (type 2 strain 16681) was amplified in C6/36 mosquito cells55. DENV of different serotypes (DENV-1: DN8700828; DENV-2: DN454009A; DENV-3: DN8700829A; DENV-4: S9201818) were obtained from the Centers for Disease Control, Department of Health, Taiwan.…”
Section: Methodsmentioning
confidence: 99%
“…DENV (type 2 strain 16681) was amplified in C6/36 mosquito cells55. DENV of different serotypes (DENV-1: DN8700828; DENV-2: DN454009A; DENV-3: DN8700829A; DENV-4: S9201818) were obtained from the Centers for Disease Control, Department of Health, Taiwan.…”
Section: Methodsmentioning
confidence: 99%
“…Different serotype of DENV (DENV-1: DN8700828; DENV-2: DN454009A; DENV-3: DN8700829A; DENV-4: S9201818) were obtained from the Centers of Disease Control, Department of Health, Taiwan. DENV was amplified in C6/36 cells41.…”
Section: Methodsmentioning
confidence: 99%
“…Recently, the same group reported that the lethal NS4B mutant replicon cannot be transcomplemented by WT NS4B protein alone but requires NS4B from the replication complex (15). To examine whether the replication-incompetent replicon SGR-Rlu-NS4B N58QN62Q could be rescued by a functionally active replicon in trans, we used the D2-Flu-SGR-Neo replicon cell line, which was originally established for an anti-DENV inhibitor study and harbors an autonomously replicating WT replicon and firefly luciferase gene (24). The NS3 helicase-defective K199AT200A mutant replicon was used as a negative control in trans-complementation assays.…”
Section: Denv Ns4b Is N Glycosylated In Virus-infected Cellsmentioning
confidence: 99%