Identification of a Specific Epitope of Haemophilus influenzae on a 16,600-Dalton Outer Membrane Protein

Murphy, Timothy F.; Nelson, Michael; Dudas, Kathleen C.; Mylotte, Joseph M.; Apicella, Michael A.

doi:10.1093/infdis/152.6.1300

Cited by 82 publications

(76 citation statements)

References 25 publications

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“…Antibody 7F3 has been characterized in detail elsewhere (13). Monoclonal antibody 3D2 recognizes an epitope on lipid A of H. influenzae; it has been described elsewhere (15 10,000 g for 20 min at 40C.…”

Section: Methodsmentioning

confidence: 99%

“…Con (28)(29)(30). Indeed, Munson and Granoff (16) The presence of P6 in the outer membrane of H. influenzae has been noted by several groups of investigators and has been the subject of recent investigation (13,14,16,33,34). It makes up a relatively small proportion of the total protein content of the outer membrane.…”

Section: Bactericidal Assaymentioning

confidence: 96%

“…2 is a Western blot assay in which a whole cell preparation and P6 preparation are tested with three antisera. It shows that P6 contains the 7F3 epitope (13) and that the preparation contains no detectable LOS using monoclonal antibody 3D2. Antibody 3D2 detects nanogram quantities of LOS in this assay (15,17 (Fig.…”

Section: Bactericidal Assaymentioning

confidence: 97%

“…A portion of the OMP is exposed on the surface of the bacterium since P6 is accessible to bactericidal antibody, monoclonal antibody 7F3 and rabbit antisera stain the organism by immunofluorescence, and absorbed antiserum immunoprecipitates P6 from whole organisms (16). P6 contains an epitope common to all strains of H. influenzae tested thus far; this epitope is a highly specific marker for H. influenzae since the epitope is absent in virtually all other bacterial species tested (13). Antibody to P6 is present in human serum as demonstrated in Western blot assay and K-ELISA.…”

Section: Bactericidal Assaymentioning

confidence: 99%

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Identification of a 16,600-dalton outer membrane protein on nontypeable Haemophilus influenzae as a target for human serum bactericidal antibody.

Murphy¹,

Bartos²,

Rice³

et al. 1986

J. Clin. Invest.

124

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A 16,600-D outer membrane protein is present in all strains of Haemophilus influenzae and antibodies to this protein are present in human serum. This study was designed to assess the role of this outer membrane protein (P6) in nontypeable H. influenzae as a target for human serum bactericidal antibody. P6 was isolated and coupled to an affinity column. Depleting normal human serum of antibodies to P6 by affinity chromatography resulted in reduced bactericidal activity of that serum for nontypeable H. influenzae. Immunopurified antibodies to P6 from human serum were bactericidal. Finally, preincubation of bacteria with a monoclonal antibody that recognizes a surface epitope on P6, inhibited human serum bactericidal killing. Taken together, these experiments establish that P6 is a target for human bactericidal antibodies. This observation provides evidence that P6 plays a potentially important role in human immunity to infection by nontypeable H. influenzae.

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Section: Methodsmentioning

confidence: 99%

Section: Bactericidal Assaymentioning

confidence: 96%

Section: Bactericidal Assaymentioning

confidence: 97%

Section: Bactericidal Assaymentioning

confidence: 99%

See 2 more Smart Citations

Identification of a 16,600-dalton outer membrane protein on nontypeable Haemophilus influenzae as a target for human serum bactericidal antibody.

Murphy¹,

Bartos²,

Rice³

et al. 1986

J. Clin. Invest.

124

View full text Add to dashboard Cite

show abstract

“…The lack of immunity to these colonizers may well be related to the inability of the immune system to sample antigenic sites such as P5 or OmpA in a way in which it can mount a response to prevent infection when innate mechanisms temporarily break down at the mucosal surface. The interstrain variation of P5 seen in NTHi (136) (10,115,143,145). It elicits bactericidal antibody in both its native (135,141) and recombinant (71) forms.…”

Section: Outer Membrane Proteinsmentioning

confidence: 99%

Lower Respiratory Tract Infections

Carroll

Adams

2016

Microbiol Spectr

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This review will focus on the infectious etiologies and more common noninfectious causes of lower respiratory tract syndromes among major immunosuppressed populations. The changing epidemiology of infections in the era of highly active antiretroviral therapy (HAART) in the case of HIV-positive patients and the impacts of both newer immune-suppressant therapies and anti-infective prophylaxis for other immunocompromised hosts will be discussed, with emphasis on diagnostic approaches and practice algorithms.

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Bacterial Antigens and Neutrophil Granule Proteins in Middle Ear Effusions

Miller

Koltai²,

Hetherington³

1990

Archives of Otolaryngology - Head and Neck Surgery

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Otitis media with effusion is a significant cause of hearing loss in young children. We hypothesized that persistent bacterial antigens in middle ear effusions (MEEs) might act as chronic inflammatory stimuli causing release of neutrophil proteins. Concentrations of neutrophil lactoferrin and a 37-kd cationic bactericidal protein (CAP 37) were measured in 47 MEEs collected from 27 children at the time of tympanostomy tube placement. Antigens of Streptococcus pneumoniae were detected by latex particle agglutination and those of Haemophilus influenzae by dot-blot assay. Bacterial antigens were detectable in 24 (51%) of MEEs: S pneumoniae in 10 (21%), H influenzae in 12 (26%), and both antigens in 2 (4%). Concentrations of lactoferrin and CAP 37 in H influenzae antigen-positive MEEs were significantly higher than in either S pneumoniae antigen-positive or antigen-negative MEEs. We conclude that H influenzae antigen causes a greater middle-ear inflammatory response, as judged by neutrophil products, than does S pneumoniae antigen.

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Identification of a Specific Epitope of Haemophilus influenzae on a 16,600-Dalton Outer Membrane Protein

Cited by 82 publications

References 25 publications

Identification of a 16,600-dalton outer membrane protein on nontypeable Haemophilus influenzae as a target for human serum bactericidal antibody.

Identification of a 16,600-dalton outer membrane protein on nontypeable Haemophilus influenzae as a target for human serum bactericidal antibody.

Lower Respiratory Tract Infections

Bacterial Antigens and Neutrophil Granule Proteins in Middle Ear Effusions

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