Identification of a Specific Leukocyte Antigen: Another Presumed Example of 5^b

Abstract

A leukocyte agglutinating antibody obtained from a multiparous normal female was studied. By means of cross‐absorption and antibody elution methods, it was established that this antibody detected a single leukocyte antigen, designated as “Ke.” The data suggest a gene frequency of 0.8 for “Ke” and 0.2 for “ke,” the postulated allelic gene. The antigen is shared by granulocytes, lymphocytes‐monocytes, eosinophils and also platelets, but is absent from red cells. The cross reaction studies indicate that this antigen may be identical with group 5b already described by Van Rood.

An isologous antigen-antibody reaction with human neutrophiles, related to neonatal neutropenia.

“…In preliminary experiments-, the separated blood cells were adjusted to a "leukocrit" or "thrombocrit" of 15%o, a concentration that had been satisfactory in the study of Ke antibody (10). It soon became evident that with this antigen concentration, DeR antibody was only partially absorbed; for complete absorption, it was necessary to use packed cells.…”

“…The third plasma, containing a monospecific antibody * Submitted for publication November 26, 1965; accepted August 1, 1966. Supported by U. S. Public Health Service grant HE 10036-01. Presented (10), was also obtained from a multiparous female, none of whose children ever presented any evidence of neutropenia. This antibody had previously been shown to detect an antigen common to leukocytes, platelets, and several solid tissues.…”

“…Leukocyte antigen-antibody reactions were studied., by a previously described agglutination method (10), in which serial twofold dilutions of all the test plasmas -were prepared in 0.1-ml vol, with isotonic saline as the diluent. To each dilution, 0.05 ml of leukocyte suspension was added.…”

“…The strength of the agglutination reaction was graded as previously described (10), and the antibody titer was determined as the highest dilution of the test plasma that produced agglutination.…”

Human neutrophil alloantigens systems

“…FREQUENCY HNA-3a is a high frequency antigen with reported phenotype frequencies ranging from 89-99% in Europeans (van Leeuwen et al 1964, de Haas et al 2000, Lalezari and Bernard 1965. Using flow cytometry technique, we found a HNA-3a frequency of 95% in randomly investigated healthy Brazilian blood donors (Table IV).…”